Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF76

Experiment: Ying_AminoAcid20 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF74 and GFF75 overlap by 4 nucleotidesGFF75 and GFF76 are separated by 194 nucleotidesGFF76 and GFF77 are separated by 91 nucleotides GFF74 - Predicted secretion system X pseudopilin PulG-like, at 73,537 to 74,055 GFF74 GFF75 - Transcription regulator like protein EpsA, at 74,052 to 74,840 GFF75 GFF76 - hypothetical protein, at 75,035 to 75,880 GFF76 GFF77 - hypothetical protein, at 75,972 to 77,546 GFF77 Position (kb) 75 76Strain fitness (log2 ratio) -1 0 1at 74.937 kb on - strandat 75.149 kb on + strand, within GFF76at 75.284 kb on + strand, within GFF76at 75.296 kb on + strand, within GFF76at 75.296 kb on + strand, within GFF76at 75.297 kb on - strand, within GFF76at 75.569 kb on + strand, within GFF76at 75.569 kb on + strand, within GFF76at 75.572 kb on + strand, within GFF76at 75.573 kb on - strand, within GFF76at 75.662 kb on + strand, within GFF76at 75.810 kb on - strandat 76.169 kb on - strand, within GFF77at 76.169 kb on - strand, within GFF77at 76.207 kb on - strand, within GFF77at 76.207 kb on - strand, within GFF77at 76.207 kb on - strand, within GFF77at 76.269 kb on + strand, within GFF77at 76.336 kb on - strand, within GFF77at 76.336 kb on - strand, within GFF77at 76.369 kb on - strand, within GFF77

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2
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74,937 - -1.7
75,149 + GFF76 0.13 +0.5
75,284 + GFF76 0.29 +0.1
75,296 + GFF76 0.31 -0.3
75,296 + GFF76 0.31 +0.1
75,297 - GFF76 0.31 -0.1
75,569 + GFF76 0.63 -0.1
75,569 + GFF76 0.63 -1.1
75,572 + GFF76 0.63 +0.3
75,573 - GFF76 0.64 +0.4
75,662 + GFF76 0.74 -0.7
75,810 - +1.3
76,169 - GFF77 0.13 +0.0
76,169 - GFF77 0.13 +0.5
76,207 - GFF77 0.15 +0.0
76,207 - GFF77 0.15 -0.5
76,207 - GFF77 0.15 +0.1
76,269 + GFF77 0.19 +0.2
76,336 - GFF77 0.23 +0.6
76,336 - GFF77 0.23 +0.5
76,369 - GFF77 0.25 +0.2

Or see this region's nucleotide sequence