Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5305

Experiment: Ying_AminoAcid20 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5302 and GFF5303 are separated by 423 nucleotidesGFF5303 and GFF5304 overlap by 4 nucleotidesGFF5304 and GFF5305 are separated by 9 nucleotidesGFF5305 and GFF5306 are separated by 46 nucleotidesGFF5306 and GFF5307 are separated by 44 nucleotidesGFF5307 and GFF5308 overlap by 4 nucleotidesGFF5308 and GFF5309 overlap by 4 nucleotides GFF5302 - hypothetical protein, at 2,315,458 to 2,315,640 GFF5302 GFF5303 - hypothetical protein, at 2,316,064 to 2,316,327 GFF5303 GFF5304 - hypothetical protein, at 2,316,324 to 2,316,617 GFF5304 GFF5305 - gene 66 protein, at 2,316,627 to 2,317,052 GFF5305 GFF5306 - hypothetical protein, at 2,317,099 to 2,317,473 GFF5306 GFF5307 - hypothetical protein, at 2,317,518 to 2,317,730 GFF5307 GFF5308 - hypothetical protein, at 2,317,727 to 2,317,963 GFF5308 GFF5309 - hypothetical protein, at 2,317,960 to 2,318,475 GFF5309 Position (kb) 2316 2317 2318Strain fitness (log2 ratio) -1 0 1at 2315.646 kb on - strandat 2315.660 kb on + strandat 2315.661 kb on - strandat 2316.563 kb on - strand, within GFF5304at 2316.571 kb on + strand, within GFF5304at 2316.571 kb on + strand, within GFF5304at 2316.667 kb on + strandat 2316.928 kb on + strand, within GFF5305at 2317.461 kb on - strandat 2317.502 kb on - strandat 2317.782 kb on + strand, within GFF5308at 2317.783 kb on - strand, within GFF5308at 2318.051 kb on + strand, within GFF5309

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2
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2,315,646 - -0.1
2,315,660 + +0.9
2,315,661 - -1.4
2,316,563 - GFF5304 0.81 +0.0
2,316,571 + GFF5304 0.84 +0.6
2,316,571 + GFF5304 0.84 +0.6
2,316,667 + -0.6
2,316,928 + GFF5305 0.71 -0.2
2,317,461 - -0.3
2,317,502 - -0.7
2,317,782 + GFF5308 0.23 +0.4
2,317,783 - GFF5308 0.24 -1.3
2,318,051 + GFF5309 0.18 -0.0

Or see this region's nucleotide sequence