Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3980

Experiment: Ying_AminoAcid20 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3978 and GFF3979 overlap by 8 nucleotidesGFF3979 and GFF3980 are separated by 11 nucleotidesGFF3980 and GFF3981 overlap by 4 nucleotidesGFF3981 and GFF3982 overlap by 4 nucleotides GFF3978 - Periplasmic binding protein, at 894,073 to 894,894 GFF3978 GFF3979 - Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 894,887 to 895,441 GFF3979 GFF3980 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 895,453 to 896,322 GFF3980 GFF3981 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 896,319 to 897,323 GFF3981 GFF3982 - Iron(III) dicitrate transport ATP-binding protein FecE (TC 3.A.1.14.1), at 897,320 to 898,081 GFF3982 Position (kb) 895 896 897Strain fitness (log2 ratio) -1 0 1at 895.657 kb on + strand, within GFF3980at 895.979 kb on - strand, within GFF3980at 895.979 kb on - strand, within GFF3980at 896.104 kb on + strand, within GFF3980at 896.104 kb on + strand, within GFF3980at 897.274 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2
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895,657 + GFF3980 0.23 -1.3
895,979 - GFF3980 0.60 -1.4
895,979 - GFF3980 0.60 +0.7
896,104 + GFF3980 0.75 -0.5
896,104 + GFF3980 0.75 -0.4
897,274 - -1.1

Or see this region's nucleotide sequence