Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF21 and GFF22 are separated by 47 nucleotides GFF22 and GFF23 are separated by 12 nucleotides GFF23 and GFF24 overlap by 4 nucleotides GFF24 and GFF25 are separated by 26 nucleotides
GFF21 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 19,242 to 20,258
GFF21
GFF22 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 20,306 to 21,064
GFF22
GFF23 - ABC transporter permease protein with unknown substrate, at 21,077 to 21,865
GFF23
GFF24 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 21,862 to 22,620
GFF24
GFF25 - putative monooxygenase, at 22,647 to 24,191
GFF25
Position (kb) 21
22 Strain fitness (log2 ratio) -1
0
1 at 21.192 kb on - strand, within GFF23 at 21.234 kb on - strand, within GFF23 at 21.234 kb on - strand, within GFF23 at 21.288 kb on - strand, within GFF23 at 21.408 kb on + strand, within GFF23 at 21.539 kb on + strand, within GFF23 at 21.786 kb on - strand, within GFF23 at 22.471 kb on + strand, within GFF24 at 22.471 kb on + strand, within GFF24 at 22.646 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 21,192 - GFF23 0.15 -0.5 21,234 - GFF23 0.20 -0.1 21,234 - GFF23 0.20 +0.3 21,288 - GFF23 0.27 +0.6 21,408 + GFF23 0.42 +0.4 21,539 + GFF23 0.59 -0.1 21,786 - GFF23 0.90 +0.2 22,471 + GFF24 0.80 -0.1 22,471 + GFF24 0.80 +1.2 22,646 + -0.2
Or see this region's nucleotide sequence