Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1934 and GFF1935 are separated by 9 nucleotides GFF1935 and GFF1936 are separated by 111 nucleotides GFF1936 and GFF1937 overlap by 17 nucleotides
GFF1934 - Rubredoxin-NAD(+) reductase (EC 1.18.1.1), at 809,254 to 810,477
GFF1934
GFF1935 - hypothetical protein, at 810,487 to 810,888
GFF1935
GFF1936 - Type III restriction-modification enzyme, helicase subunit, at 811,000 to 811,746
GFF1936
GFF1937 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 811,730 to 812,770
GFF1937
Position (kb) 810
811
812 Strain fitness (log2 ratio) -1
0
1 at 810.344 kb on + strand, within GFF1934 at 810.415 kb on + strand at 810.415 kb on + strand at 810.572 kb on + strand, within GFF1935 at 810.600 kb on - strand, within GFF1935 at 810.900 kb on + strand at 810.901 kb on - strand at 810.901 kb on - strand at 811.912 kb on - strand, within GFF1937 at 811.912 kb on - strand, within GFF1937 at 811.959 kb on + strand, within GFF1937 at 811.959 kb on + strand, within GFF1937 at 811.960 kb on - strand, within GFF1937 at 811.960 kb on - strand, within GFF1937 at 811.960 kb on - strand, within GFF1937 at 811.960 kb on - strand, within GFF1937 at 812.037 kb on + strand, within GFF1937 at 812.037 kb on + strand, within GFF1937 at 812.340 kb on + strand, within GFF1937
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 810,344 + GFF1934 0.89 +0.2 810,415 + +0.1 810,415 + -1.3 810,572 + GFF1935 0.21 -0.1 810,600 - GFF1935 0.28 +0.4 810,900 + +0.4 810,901 - -0.0 810,901 - -0.5 811,912 - GFF1937 0.17 +0.3 811,912 - GFF1937 0.17 +0.4 811,959 + GFF1937 0.22 +0.5 811,959 + GFF1937 0.22 -0.1 811,960 - GFF1937 0.22 +0.1 811,960 - GFF1937 0.22 +0.4 811,960 - GFF1937 0.22 -0.6 811,960 - GFF1937 0.22 +0.1 812,037 + GFF1937 0.29 -0.2 812,037 + GFF1937 0.29 -0.6 812,340 + GFF1937 0.59 -0.2
Or see this region's nucleotide sequence