Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF10

Experiment: Ying_AminoAcid20 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF8 and GFF9 overlap by 1 nucleotidesGFF9 and GFF10 are separated by 9 nucleotidesGFF10 and GFF11 are separated by 36 nucleotides GFF8 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 5,768 to 6,532 GFF8 GFF9 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 6,532 to 7,299 GFF9 GFF10 - N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47), at 7,309 to 8,517 GFF10 GFF11 - Acetolactate synthase large subunit (EC 2.2.1.6), at 8,554 to 9,618 GFF11 Position (kb) 7 8 9Strain fitness (log2 ratio) -1 0 1at 6.592 kb on + strandat 6.592 kb on + strandat 6.592 kb on + strandat 6.593 kb on - strandat 6.593 kb on - strandat 6.658 kb on + strand, within GFF9at 6.658 kb on + strand, within GFF9at 6.659 kb on - strand, within GFF9at 6.659 kb on - strand, within GFF9at 6.685 kb on + strand, within GFF9at 6.686 kb on - strand, within GFF9at 6.709 kb on + strand, within GFF9at 6.710 kb on - strand, within GFF9at 6.710 kb on - strand, within GFF9at 6.940 kb on + strand, within GFF9at 6.940 kb on + strand, within GFF9at 6.941 kb on - strand, within GFF9at 6.941 kb on - strand, within GFF9at 6.941 kb on - strand, within GFF9at 6.941 kb on - strand, within GFF9at 7.486 kb on + strand, within GFF10at 7.487 kb on - strand, within GFF10at 7.615 kb on + strand, within GFF10at 7.828 kb on + strand, within GFF10at 8.201 kb on - strand, within GFF10at 8.224 kb on + strand, within GFF10at 8.225 kb on - strand, within GFF10at 8.443 kb on + strandat 8.507 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2
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6,592 + +0.5
6,592 + -0.7
6,592 + +0.2
6,593 - +0.4
6,593 - +0.7
6,658 + GFF9 0.16 +0.1
6,658 + GFF9 0.16 -0.4
6,659 - GFF9 0.17 -0.7
6,659 - GFF9 0.17 -1.7
6,685 + GFF9 0.20 -0.2
6,686 - GFF9 0.20 +0.7
6,709 + GFF9 0.23 +0.1
6,710 - GFF9 0.23 +0.5
6,710 - GFF9 0.23 +0.3
6,940 + GFF9 0.53 +0.1
6,940 + GFF9 0.53 -0.4
6,941 - GFF9 0.53 -0.1
6,941 - GFF9 0.53 -0.5
6,941 - GFF9 0.53 +0.1
6,941 - GFF9 0.53 -0.9
7,486 + GFF10 0.15 +1.1
7,487 - GFF10 0.15 -0.8
7,615 + GFF10 0.25 +0.1
7,828 + GFF10 0.43 +0.2
8,201 - GFF10 0.74 -0.8
8,224 + GFF10 0.76 -0.2
8,225 - GFF10 0.76 -0.3
8,443 + -0.1
8,507 - +0.9

Or see this region's nucleotide sequence