Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF694 and GFF695 are separated by 39 nucleotides GFF695 and GFF696 overlap by 20 nucleotides GFF696 and GFF697 are separated by 1 nucleotides
GFF694 - hypothetical protein, at 374,463 to 374,696
GFF694
GFF695 - Cytochrome oxidase biogenesis protein Surf1, facilitates heme A insertion, at 374,736 to 375,476
GFF695
GFF696 - Probable transmembrane protein, at 375,457 to 376,137
GFF696
GFF697 - Heme A synthase, cytochrome oxidase biogenesis protein Cox15-CtaA, at 376,139 to 377,302
GFF697
Position (kb)
375
376
377 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 374.464 kb on + strand at 374.509 kb on + strand, within GFF694 at 374.509 kb on + strand, within GFF694 at 374.632 kb on + strand, within GFF694 at 374.632 kb on + strand, within GFF694 at 374.632 kb on + strand, within GFF694 at 374.632 kb on + strand, within GFF694 at 374.632 kb on + strand, within GFF694 at 374.633 kb on - strand, within GFF694 at 374.633 kb on - strand, within GFF694 at 374.633 kb on - strand, within GFF694 at 374.633 kb on - strand, within GFF694 at 374.689 kb on + strand at 374.689 kb on + strand at 374.689 kb on + strand at 374.690 kb on - strand at 374.690 kb on - strand at 374.690 kb on - strand at 375.187 kb on + strand, within GFF695 at 375.187 kb on + strand, within GFF695 at 375.187 kb on + strand, within GFF695 at 375.187 kb on + strand, within GFF695 at 375.188 kb on - strand, within GFF695 at 375.188 kb on - strand, within GFF695 at 375.188 kb on - strand, within GFF695 at 375.188 kb on - strand, within GFF695 at 375.188 kb on - strand, within GFF695 at 375.188 kb on - strand, within GFF695 at 375.190 kb on - strand, within GFF695 at 375.230 kb on + strand, within GFF695 at 375.230 kb on + strand, within GFF695 at 375.366 kb on + strand, within GFF695 at 375.366 kb on + strand, within GFF695 at 375.411 kb on + strand at 375.411 kb on + strand at 375.411 kb on + strand at 375.411 kb on + strand at 375.611 kb on - strand, within GFF696 at 375.619 kb on + strand, within GFF696 at 375.619 kb on + strand, within GFF696 at 375.619 kb on + strand, within GFF696 at 375.619 kb on + strand, within GFF696 at 375.619 kb on + strand, within GFF696 at 375.619 kb on + strand, within GFF696 at 375.619 kb on + strand, within GFF696 at 375.619 kb on + strand, within GFF696 at 375.620 kb on - strand, within GFF696 at 375.622 kb on + strand, within GFF696 at 375.622 kb on + strand, within GFF696 at 375.622 kb on + strand, within GFF696 at 375.622 kb on + strand, within GFF696 at 375.622 kb on + strand, within GFF696 at 375.622 kb on + strand, within GFF696 at 375.623 kb on - strand, within GFF696 at 375.623 kb on - strand, within GFF696 at 375.623 kb on - strand, within GFF696 at 375.652 kb on + strand, within GFF696 at 375.652 kb on + strand, within GFF696 at 375.652 kb on + strand, within GFF696 at 375.652 kb on + strand, within GFF696 at 375.653 kb on - strand, within GFF696 at 375.805 kb on + strand, within GFF696 at 375.805 kb on + strand, within GFF696 at 375.806 kb on - strand, within GFF696 at 376.003 kb on + strand, within GFF696 at 376.003 kb on + strand, within GFF696 at 376.003 kb on + strand, within GFF696 at 376.003 kb on + strand, within GFF696 at 376.003 kb on + strand, within GFF696 at 376.003 kb on + strand, within GFF696 at 376.003 kb on + strand, within GFF696 at 376.004 kb on - strand, within GFF696 at 376.004 kb on - strand, within GFF696 at 376.157 kb on + strand at 376.157 kb on + strand at 376.157 kb on + strand at 376.594 kb on - strand, within GFF697 at 376.594 kb on - strand, within GFF697 at 376.721 kb on + strand, within GFF697 at 376.847 kb on + strand, within GFF697 at 376.847 kb on + strand, within GFF697 at 376.847 kb on + strand, within GFF697 at 376.848 kb on - strand, within GFF697 at 376.848 kb on - strand, within GFF697 at 376.848 kb on - strand, within GFF697 at 376.997 kb on + strand, within GFF697 at 376.997 kb on + strand, within GFF697 at 376.998 kb on - strand, within GFF697 at 377.018 kb on + strand, within GFF697 at 377.018 kb on + strand, within GFF697 at 377.019 kb on - strand, within GFF697
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 374,464 + -0.2 374,509 + GFF694 0.20 -0.1 374,509 + GFF694 0.20 -0.2 374,632 + GFF694 0.72 -0.3 374,632 + GFF694 0.72 +0.3 374,632 + GFF694 0.72 +0.2 374,632 + GFF694 0.72 +0.6 374,632 + GFF694 0.72 -1.5 374,633 - GFF694 0.73 -0.2 374,633 - GFF694 0.73 +1.0 374,633 - GFF694 0.73 +0.9 374,633 - GFF694 0.73 -0.4 374,689 + -0.6 374,689 + -0.2 374,689 + +0.2 374,690 - -0.1 374,690 - -1.3 374,690 - +0.6 375,187 + GFF695 0.61 -1.1 375,187 + GFF695 0.61 -1.1 375,187 + GFF695 0.61 -2.3 375,187 + GFF695 0.61 -1.3 375,188 - GFF695 0.61 -0.5 375,188 - GFF695 0.61 +0.5 375,188 - GFF695 0.61 -0.3 375,188 - GFF695 0.61 -1.6 375,188 - GFF695 0.61 -1.9 375,188 - GFF695 0.61 -1.3 375,190 - GFF695 0.61 -0.9 375,230 + GFF695 0.67 -0.6 375,230 + GFF695 0.67 -1.3 375,366 + GFF695 0.85 -1.3 375,366 + GFF695 0.85 -1.9 375,411 + +0.5 375,411 + -1.5 375,411 + -1.3 375,411 + +0.9 375,611 - GFF696 0.23 -0.6 375,619 + GFF696 0.24 -0.6 375,619 + GFF696 0.24 -2.0 375,619 + GFF696 0.24 -2.6 375,619 + GFF696 0.24 -3.3 375,619 + GFF696 0.24 -2.1 375,619 + GFF696 0.24 -1.8 375,619 + GFF696 0.24 -0.9 375,619 + GFF696 0.24 -0.6 375,620 - GFF696 0.24 -0.9 375,622 + GFF696 0.24 -2.9 375,622 + GFF696 0.24 -1.3 375,622 + GFF696 0.24 -1.7 375,622 + GFF696 0.24 -0.9 375,622 + GFF696 0.24 -0.8 375,622 + GFF696 0.24 -1.4 375,623 - GFF696 0.24 +0.1 375,623 - GFF696 0.24 -1.7 375,623 - GFF696 0.24 -1.3 375,652 + GFF696 0.29 -3.2 375,652 + GFF696 0.29 -2.1 375,652 + GFF696 0.29 +0.1 375,652 + GFF696 0.29 -4.3 375,653 - GFF696 0.29 -1.0 375,805 + GFF696 0.51 -2.0 375,805 + GFF696 0.51 +0.7 375,806 - GFF696 0.51 +0.2 376,003 + GFF696 0.80 -0.3 376,003 + GFF696 0.80 -2.6 376,003 + GFF696 0.80 -1.5 376,003 + GFF696 0.80 -1.0 376,003 + GFF696 0.80 -0.4 376,003 + GFF696 0.80 -0.2 376,003 + GFF696 0.80 -1.9 376,004 - GFF696 0.80 -1.5 376,004 - GFF696 0.80 -1.1 376,157 + -3.5 376,157 + -2.1 376,157 + -1.9 376,594 - GFF697 0.39 -2.5 376,594 - GFF697 0.39 -2.0 376,721 + GFF697 0.50 -2.3 376,847 + GFF697 0.61 -1.8 376,847 + GFF697 0.61 -2.5 376,847 + GFF697 0.61 -2.0 376,848 - GFF697 0.61 -1.3 376,848 - GFF697 0.61 -0.5 376,848 - GFF697 0.61 -1.6 376,997 + GFF697 0.74 +0.7 376,997 + GFF697 0.74 -2.6 376,998 - GFF697 0.74 -0.4 377,018 + GFF697 0.76 -1.3 377,018 + GFF697 0.76 -1.1 377,019 - GFF697 0.76 -2.7
Or see this region's nucleotide sequence