Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF599 and GFF600 are separated by 7 nucleotides GFF600 and GFF601 are separated by 4 nucleotides GFF601 and GFF602 are separated by 91 nucleotides GFF602 and GFF603 overlap by 4 nucleotides
GFF599 - Signal recognition particle, subunit Ffh SRP54 (TC 3.A.5.1.1), at 273,491 to 274,861
GFF599
GFF600 - Phosphohydrolase (MutT/nudix family protein), at 274,869 to 275,486
GFF600
GFF601 - Transcriptional regulator, IclR family, at 275,491 to 276,252
GFF601
GFF602 - Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-), at 276,344 to 277,159
GFF602
GFF603 - Monooxygenase, flavin-binding family, at 277,156 to 278,469
GFF603
Position (kb)
275
276
277 Strain fitness (log2 ratio)
-1
0
1 at 274.978 kb on - strand, within GFF600 at 275.442 kb on + strand at 275.481 kb on + strand at 275.481 kb on + strand at 275.482 kb on - strand at 275.909 kb on + strand, within GFF601 at 275.909 kb on + strand, within GFF601 at 275.909 kb on + strand, within GFF601 at 275.909 kb on + strand, within GFF601 at 275.910 kb on - strand, within GFF601 at 275.910 kb on - strand, within GFF601 at 276.060 kb on - strand, within GFF601 at 276.309 kb on + strand at 276.806 kb on + strand, within GFF602 at 276.836 kb on + strand, within GFF602 at 276.836 kb on + strand, within GFF602 at 276.902 kb on + strand, within GFF602 at 276.903 kb on - strand, within GFF602
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 274,978 - GFF600 0.18 +0.7 275,442 + -0.0 275,481 + -0.7 275,481 + +0.2 275,482 - +0.2 275,909 + GFF601 0.55 -0.3 275,909 + GFF601 0.55 +0.1 275,909 + GFF601 0.55 +0.2 275,909 + GFF601 0.55 -0.0 275,910 - GFF601 0.55 -0.2 275,910 - GFF601 0.55 -0.7 276,060 - GFF601 0.75 +0.2 276,309 + +0.3 276,806 + GFF602 0.57 +0.1 276,836 + GFF602 0.60 -0.1 276,836 + GFF602 0.60 +0.2 276,902 + GFF602 0.68 +0.6 276,903 - GFF602 0.69 -0.4
Or see this region's nucleotide sequence