Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5474 and GFF5475 are separated by 42 nucleotides GFF5475 and GFF5476 overlap by 4 nucleotides GFF5476 and GFF5477 are separated by 15 nucleotides GFF5477 and GFF5478 are separated by 15 nucleotides
GFF5474 - Epoxyqueuosine (oQ) reductase QueG, at 2,476,473 to 2,477,564
GFF5474
GFF5475 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 2,477,607 to 2,478,143
GFF5475
GFF5476 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,478,140 to 2,479,597
GFF5476
GFF5477 - Probable ferredoxin subunit of a ring-hydroxylating dioxygenase oxidoreductase protein (EC 1.-.-.-), at 2,479,613 to 2,479,942
GFF5477
GFF5478 - Tricarboxylate transport protein TctC, at 2,479,958 to 2,480,926
GFF5478
Position (kb)
2478
2479
2480 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2477.218 kb on + strand, within GFF5474 at 2477.219 kb on - strand, within GFF5474 at 2477.236 kb on + strand, within GFF5474 at 2477.236 kb on + strand, within GFF5474 at 2477.237 kb on - strand, within GFF5474 at 2477.237 kb on - strand, within GFF5474 at 2477.320 kb on + strand, within GFF5474 at 2477.320 kb on + strand, within GFF5474 at 2477.320 kb on + strand, within GFF5474 at 2477.320 kb on + strand, within GFF5474 at 2477.404 kb on + strand, within GFF5474 at 2477.404 kb on + strand, within GFF5474 at 2477.404 kb on + strand, within GFF5474 at 2477.404 kb on + strand, within GFF5474 at 2477.404 kb on + strand, within GFF5474 at 2477.405 kb on - strand, within GFF5474 at 2477.405 kb on - strand, within GFF5474 at 2477.405 kb on - strand, within GFF5474 at 2477.524 kb on - strand at 2477.578 kb on + strand at 2477.578 kb on + strand at 2477.579 kb on - strand at 2477.579 kb on - strand at 2478.264 kb on - strand at 2478.525 kb on - strand, within GFF5476 at 2478.552 kb on - strand, within GFF5476 at 2479.172 kb on + strand, within GFF5476 at 2479.172 kb on + strand, within GFF5476 at 2479.173 kb on - strand, within GFF5476 at 2479.508 kb on + strand at 2479.509 kb on - strand at 2479.553 kb on + strand at 2479.836 kb on + strand, within GFF5477 at 2479.836 kb on + strand, within GFF5477 at 2479.837 kb on - strand, within GFF5477 at 2480.067 kb on + strand, within GFF5478 at 2480.067 kb on + strand, within GFF5478 at 2480.068 kb on - strand, within GFF5478 at 2480.172 kb on + strand, within GFF5478 at 2480.173 kb on - strand, within GFF5478 at 2480.476 kb on - strand, within GFF5478 at 2480.476 kb on - strand, within GFF5478
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 2,477,218 + GFF5474 0.68 +0.2 2,477,219 - GFF5474 0.68 -0.4 2,477,236 + GFF5474 0.70 +0.2 2,477,236 + GFF5474 0.70 +0.6 2,477,237 - GFF5474 0.70 -0.7 2,477,237 - GFF5474 0.70 -0.3 2,477,320 + GFF5474 0.78 -1.1 2,477,320 + GFF5474 0.78 +0.7 2,477,320 + GFF5474 0.78 +0.5 2,477,320 + GFF5474 0.78 -0.4 2,477,404 + GFF5474 0.85 -1.0 2,477,404 + GFF5474 0.85 +0.1 2,477,404 + GFF5474 0.85 +1.7 2,477,404 + GFF5474 0.85 -0.6 2,477,404 + GFF5474 0.85 +0.7 2,477,405 - GFF5474 0.85 +0.4 2,477,405 - GFF5474 0.85 +0.6 2,477,405 - GFF5474 0.85 +0.0 2,477,524 - -3.3 2,477,578 + -1.4 2,477,578 + +0.3 2,477,579 - -0.7 2,477,579 - +0.2 2,478,264 - +0.9 2,478,525 - GFF5476 0.26 +0.9 2,478,552 - GFF5476 0.28 +1.7 2,479,172 + GFF5476 0.71 -0.8 2,479,172 + GFF5476 0.71 -0.3 2,479,173 - GFF5476 0.71 -0.2 2,479,508 + -1.7 2,479,509 - +1.1 2,479,553 + -0.1 2,479,836 + GFF5477 0.68 +0.9 2,479,836 + GFF5477 0.68 +0.6 2,479,837 - GFF5477 0.68 +0.1 2,480,067 + GFF5478 0.11 +0.4 2,480,067 + GFF5478 0.11 -0.2 2,480,068 - GFF5478 0.11 -0.4 2,480,172 + GFF5478 0.22 -0.1 2,480,173 - GFF5478 0.22 +0.2 2,480,476 - GFF5478 0.53 +0.2 2,480,476 - GFF5478 0.53 -0.8
Or see this region's nucleotide sequence