Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5476

Experiment: Ying_AminoAcid20 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5474 and GFF5475 are separated by 42 nucleotidesGFF5475 and GFF5476 overlap by 4 nucleotidesGFF5476 and GFF5477 are separated by 15 nucleotidesGFF5477 and GFF5478 are separated by 15 nucleotides GFF5474 - Epoxyqueuosine (oQ) reductase QueG, at 2,476,473 to 2,477,564 GFF5474 GFF5475 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 2,477,607 to 2,478,143 GFF5475 GFF5476 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,478,140 to 2,479,597 GFF5476 GFF5477 - Probable ferredoxin subunit of a ring-hydroxylating dioxygenase oxidoreductase protein (EC 1.-.-.-), at 2,479,613 to 2,479,942 GFF5477 GFF5478 - Tricarboxylate transport protein TctC, at 2,479,958 to 2,480,926 GFF5478 Position (kb) 2478 2479 2480Strain fitness (log2 ratio) -3 -2 -1 0 1at 2477.218 kb on + strand, within GFF5474at 2477.219 kb on - strand, within GFF5474at 2477.236 kb on + strand, within GFF5474at 2477.236 kb on + strand, within GFF5474at 2477.237 kb on - strand, within GFF5474at 2477.237 kb on - strand, within GFF5474at 2477.320 kb on + strand, within GFF5474at 2477.320 kb on + strand, within GFF5474at 2477.320 kb on + strand, within GFF5474at 2477.320 kb on + strand, within GFF5474at 2477.404 kb on + strand, within GFF5474at 2477.404 kb on + strand, within GFF5474at 2477.404 kb on + strand, within GFF5474at 2477.404 kb on + strand, within GFF5474at 2477.404 kb on + strand, within GFF5474at 2477.405 kb on - strand, within GFF5474at 2477.405 kb on - strand, within GFF5474at 2477.405 kb on - strand, within GFF5474at 2477.524 kb on - strandat 2477.578 kb on + strandat 2477.578 kb on + strandat 2477.579 kb on - strandat 2477.579 kb on - strandat 2478.264 kb on - strandat 2478.525 kb on - strand, within GFF5476at 2478.552 kb on - strand, within GFF5476at 2479.172 kb on + strand, within GFF5476at 2479.172 kb on + strand, within GFF5476at 2479.173 kb on - strand, within GFF5476at 2479.508 kb on + strandat 2479.509 kb on - strandat 2479.553 kb on + strandat 2479.836 kb on + strand, within GFF5477at 2479.836 kb on + strand, within GFF5477at 2479.837 kb on - strand, within GFF5477at 2480.067 kb on + strand, within GFF5478at 2480.067 kb on + strand, within GFF5478at 2480.068 kb on - strand, within GFF5478at 2480.172 kb on + strand, within GFF5478at 2480.173 kb on - strand, within GFF5478at 2480.476 kb on - strand, within GFF5478at 2480.476 kb on - strand, within GFF5478

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2
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2,477,218 + GFF5474 0.68 +0.2
2,477,219 - GFF5474 0.68 -0.4
2,477,236 + GFF5474 0.70 +0.2
2,477,236 + GFF5474 0.70 +0.6
2,477,237 - GFF5474 0.70 -0.7
2,477,237 - GFF5474 0.70 -0.3
2,477,320 + GFF5474 0.78 -1.1
2,477,320 + GFF5474 0.78 +0.7
2,477,320 + GFF5474 0.78 +0.5
2,477,320 + GFF5474 0.78 -0.4
2,477,404 + GFF5474 0.85 -1.0
2,477,404 + GFF5474 0.85 +0.1
2,477,404 + GFF5474 0.85 +1.7
2,477,404 + GFF5474 0.85 -0.6
2,477,404 + GFF5474 0.85 +0.7
2,477,405 - GFF5474 0.85 +0.4
2,477,405 - GFF5474 0.85 +0.6
2,477,405 - GFF5474 0.85 +0.0
2,477,524 - -3.3
2,477,578 + -1.4
2,477,578 + +0.3
2,477,579 - -0.7
2,477,579 - +0.2
2,478,264 - +0.9
2,478,525 - GFF5476 0.26 +0.9
2,478,552 - GFF5476 0.28 +1.7
2,479,172 + GFF5476 0.71 -0.8
2,479,172 + GFF5476 0.71 -0.3
2,479,173 - GFF5476 0.71 -0.2
2,479,508 + -1.7
2,479,509 - +1.1
2,479,553 + -0.1
2,479,836 + GFF5477 0.68 +0.9
2,479,836 + GFF5477 0.68 +0.6
2,479,837 - GFF5477 0.68 +0.1
2,480,067 + GFF5478 0.11 +0.4
2,480,067 + GFF5478 0.11 -0.2
2,480,068 - GFF5478 0.11 -0.4
2,480,172 + GFF5478 0.22 -0.1
2,480,173 - GFF5478 0.22 +0.2
2,480,476 - GFF5478 0.53 +0.2
2,480,476 - GFF5478 0.53 -0.8

Or see this region's nucleotide sequence