Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5253 and GFF5254 overlap by 4 nucleotides GFF5254 and GFF5255 are separated by 19 nucleotides GFF5255 and GFF5256 overlap by 4 nucleotides
GFF5253 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,268,923 to 2,270,455
GFF5253
GFF5254 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 2,270,452 to 2,271,390
GFF5254
GFF5255 - ABC transporter substrate-binding protein, at 2,271,410 to 2,272,357
GFF5255
GFF5256 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 2,272,354 to 2,273,133
GFF5256
Position (kb)
2270
2271
2272 Strain fitness (log2 ratio)
-1
0
1 at 2269.454 kb on + strand, within GFF5253 at 2269.455 kb on - strand, within GFF5253 at 2269.499 kb on + strand, within GFF5253 at 2269.533 kb on - strand, within GFF5253 at 2270.157 kb on - strand, within GFF5253 at 2270.384 kb on + strand at 2270.827 kb on + strand, within GFF5254 at 2270.828 kb on - strand, within GFF5254 at 2270.887 kb on + strand, within GFF5254 at 2270.887 kb on + strand, within GFF5254 at 2271.131 kb on - strand, within GFF5254 at 2271.389 kb on + strand at 2271.389 kb on - strand at 2271.741 kb on - strand, within GFF5255 at 2271.741 kb on - strand, within GFF5255 at 2271.741 kb on - strand, within GFF5255 at 2271.741 kb on - strand, within GFF5255 at 2271.896 kb on + strand, within GFF5255 at 2272.034 kb on + strand, within GFF5255 at 2272.034 kb on + strand, within GFF5255 at 2272.038 kb on - strand, within GFF5255 at 2272.202 kb on + strand, within GFF5255 at 2272.202 kb on + strand, within GFF5255 at 2272.203 kb on - strand, within GFF5255 at 2272.349 kb on + strand at 2272.349 kb on + strand at 2272.350 kb on - strand at 2272.350 kb on - strand at 2272.350 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 2,269,454 + GFF5253 0.35 -0.3 2,269,455 - GFF5253 0.35 +0.0 2,269,499 + GFF5253 0.38 -0.3 2,269,533 - GFF5253 0.40 +0.3 2,270,157 - GFF5253 0.80 -1.1 2,270,384 + +0.2 2,270,827 + GFF5254 0.40 -0.3 2,270,828 - GFF5254 0.40 -0.5 2,270,887 + GFF5254 0.46 -1.2 2,270,887 + GFF5254 0.46 +0.5 2,271,131 - GFF5254 0.72 +0.8 2,271,389 + +0.0 2,271,389 - +0.2 2,271,741 - GFF5255 0.35 +0.3 2,271,741 - GFF5255 0.35 -1.5 2,271,741 - GFF5255 0.35 -0.0 2,271,741 - GFF5255 0.35 -0.0 2,271,896 + GFF5255 0.51 +0.0 2,272,034 + GFF5255 0.66 +0.7 2,272,034 + GFF5255 0.66 -1.1 2,272,038 - GFF5255 0.66 +0.0 2,272,202 + GFF5255 0.84 -0.1 2,272,202 + GFF5255 0.84 +0.1 2,272,203 - GFF5255 0.84 +0.2 2,272,349 + +0.6 2,272,349 + -0.5 2,272,350 - +0.6 2,272,350 - +0.2 2,272,350 - -1.3
Or see this region's nucleotide sequence