Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3655 and GFF3656 are separated by 96 nucleotides GFF3656 and GFF3657 are separated by 109 nucleotides GFF3657 and GFF3658 are separated by 87 nucleotides GFF3658 and GFF3659 overlap by 43 nucleotides
GFF3655 - TldE protein, part of TldE/TldD proteolytic complex, at 540,859 to 542,274
GFF3655
GFF3656 - Transamidase GatB domain protein, at 542,371 to 542,820
GFF3656
GFF3657 - SSU ribosomal protein S21p, at 542,930 to 543,142
GFF3657
GFF3658 - hypothetical protein, at 543,230 to 543,436
GFF3658
GFF3659 - NAD(FAD)-utilizing dehydrogenases, at 543,394 to 544,596
GFF3659
Position (kb) 542
543 Strain fitness (log2 ratio) -2
-1
0
1 at 541.528 kb on + strand, within GFF3655 at 541.529 kb on - strand, within GFF3655 at 541.529 kb on - strand, within GFF3655 at 541.529 kb on - strand, within GFF3655 at 541.582 kb on + strand, within GFF3655 at 541.687 kb on + strand, within GFF3655 at 541.687 kb on + strand, within GFF3655 at 541.888 kb on + strand, within GFF3655 at 541.889 kb on - strand, within GFF3655 at 541.903 kb on + strand, within GFF3655 at 541.903 kb on + strand, within GFF3655 at 541.903 kb on + strand, within GFF3655 at 542.068 kb on + strand, within GFF3655 at 542.068 kb on + strand, within GFF3655 at 542.068 kb on + strand, within GFF3655 at 542.068 kb on + strand, within GFF3655 at 542.069 kb on - strand, within GFF3655 at 542.069 kb on - strand, within GFF3655 at 542.069 kb on - strand, within GFF3655 at 542.069 kb on - strand, within GFF3655 at 542.069 kb on - strand, within GFF3655 at 542.069 kb on - strand, within GFF3655 at 542.069 kb on - strand, within GFF3655 at 542.084 kb on - strand, within GFF3655 at 542.209 kb on + strand at 542.210 kb on - strand at 542.210 kb on - strand at 542.210 kb on - strand at 542.215 kb on + strand at 542.216 kb on - strand at 542.216 kb on - strand at 542.216 kb on - strand at 542.216 kb on - strand at 542.216 kb on - strand at 542.216 kb on - strand at 542.272 kb on + strand at 542.272 kb on + strand at 542.273 kb on - strand at 542.273 kb on - strand at 542.555 kb on + strand, within GFF3656 at 542.555 kb on + strand, within GFF3656 at 542.555 kb on + strand, within GFF3656 at 542.555 kb on + strand, within GFF3656 at 542.555 kb on + strand, within GFF3656 at 542.555 kb on + strand, within GFF3656 at 542.555 kb on + strand, within GFF3656 at 542.555 kb on + strand, within GFF3656 at 542.556 kb on - strand, within GFF3656 at 542.556 kb on - strand, within GFF3656 at 542.934 kb on + strand at 542.934 kb on + strand at 542.935 kb on - strand at 542.935 kb on - strand at 542.935 kb on - strand at 543.274 kb on - strand, within GFF3658 at 543.274 kb on - strand, within GFF3658 at 543.446 kb on + strand at 543.446 kb on + strand at 543.447 kb on - strand at 543.447 kb on - strand at 543.491 kb on + strand at 543.491 kb on + strand at 543.491 kb on + strand at 543.491 kb on + strand at 543.491 kb on + strand at 543.492 kb on - strand at 543.492 kb on - strand at 543.492 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 541,528 + GFF3655 0.47 +0.2 541,529 - GFF3655 0.47 +0.8 541,529 - GFF3655 0.47 -1.1 541,529 - GFF3655 0.47 -0.2 541,582 + GFF3655 0.51 -0.6 541,687 + GFF3655 0.58 -1.6 541,687 + GFF3655 0.58 -1.2 541,888 + GFF3655 0.73 -0.5 541,889 - GFF3655 0.73 -0.5 541,903 + GFF3655 0.74 -0.4 541,903 + GFF3655 0.74 +0.2 541,903 + GFF3655 0.74 +0.4 542,068 + GFF3655 0.85 -0.8 542,068 + GFF3655 0.85 -1.5 542,068 + GFF3655 0.85 +0.3 542,068 + GFF3655 0.85 -1.9 542,069 - GFF3655 0.85 -1.7 542,069 - GFF3655 0.85 +0.9 542,069 - GFF3655 0.85 -0.8 542,069 - GFF3655 0.85 -1.4 542,069 - GFF3655 0.85 -1.1 542,069 - GFF3655 0.85 -0.3 542,069 - GFF3655 0.85 +0.7 542,084 - GFF3655 0.87 -0.9 542,209 + +0.1 542,210 - -0.7 542,210 - -0.3 542,210 - -0.9 542,215 + -1.2 542,216 - -0.5 542,216 - +0.4 542,216 - -1.3 542,216 - -0.3 542,216 - -1.8 542,216 - +0.1 542,272 + -0.8 542,272 + -0.7 542,273 - +0.2 542,273 - -0.5 542,555 + GFF3656 0.41 +0.3 542,555 + GFF3656 0.41 +0.4 542,555 + GFF3656 0.41 -1.4 542,555 + GFF3656 0.41 -0.4 542,555 + GFF3656 0.41 -0.0 542,555 + GFF3656 0.41 -0.6 542,555 + GFF3656 0.41 -0.8 542,555 + GFF3656 0.41 +0.0 542,556 - GFF3656 0.41 -0.3 542,556 - GFF3656 0.41 -0.2 542,934 + +0.3 542,934 + -0.5 542,935 - -0.2 542,935 - +0.5 542,935 - -0.7 543,274 - GFF3658 0.21 +0.4 543,274 - GFF3658 0.21 +0.7 543,446 + -0.1 543,446 + -0.6 543,447 - -0.1 543,447 - +0.4 543,491 + +0.0 543,491 + +0.1 543,491 + +0.1 543,491 + +0.5 543,491 + +0.9 543,492 - +0.2 543,492 - +0.3 543,492 - +0.2
Or see this region's nucleotide sequence