Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3421 and GFF3422 are separated by 137 nucleotides GFF3422 and GFF3423 are separated by 125 nucleotides GFF3423 and GFF3424 are separated by 30 nucleotides
GFF3421 - Histone acetyltransferase HPA2 and related acetyltransferases, at 306,064 to 306,384
GFF3421
GFF3422 - SSU ribosomal protein S20p, at 306,522 to 306,821
GFF3422
GFF3423 - Proposed peptidoglycan lipid II flippase MurJ, at 306,947 to 308,506
GFF3423
GFF3424 - Protein sirB1, at 308,537 to 309,394
GFF3424
Position (kb) 306
307
308
309 Strain fitness (log2 ratio) -1
0
1
2 at 305.963 kb on + strand at 305.964 kb on - strand at 305.965 kb on + strand at 305.966 kb on - strand at 305.968 kb on - strand at 305.972 kb on + strand at 305.978 kb on - strand at 305.978 kb on - strand at 305.978 kb on - strand at 306.211 kb on + strand, within GFF3421 at 306.211 kb on + strand, within GFF3421 at 306.211 kb on + strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.212 kb on - strand, within GFF3421 at 306.872 kb on - strand at 308.509 kb on + strand at 308.509 kb on + strand at 308.509 kb on + strand at 308.509 kb on + strand at 308.509 kb on + strand at 308.509 kb on + strand at 308.510 kb on - strand at 308.510 kb on - strand at 308.510 kb on - strand at 308.550 kb on - strand at 308.580 kb on - strand at 308.580 kb on - strand at 308.813 kb on + strand, within GFF3424 at 308.813 kb on + strand, within GFF3424 at 308.813 kb on + strand, within GFF3424 at 308.813 kb on + strand, within GFF3424 at 308.814 kb on - strand, within GFF3424 at 308.814 kb on - strand, within GFF3424 at 308.814 kb on - strand, within GFF3424 at 308.814 kb on - strand, within GFF3424 at 308.816 kb on + strand, within GFF3424 at 308.817 kb on - strand, within GFF3424 at 308.817 kb on - strand, within GFF3424 at 308.817 kb on - strand, within GFF3424 at 308.817 kb on - strand, within GFF3424 at 308.817 kb on - strand, within GFF3424 at 308.817 kb on - strand, within GFF3424 at 309.050 kb on + strand, within GFF3424 at 309.050 kb on + strand, within GFF3424 at 309.051 kb on - strand, within GFF3424 at 309.051 kb on - strand, within GFF3424 at 309.101 kb on + strand, within GFF3424 at 309.101 kb on + strand, within GFF3424 at 309.101 kb on + strand, within GFF3424 at 309.101 kb on + strand, within GFF3424 at 309.314 kb on + strand at 309.314 kb on + strand at 309.315 kb on - strand at 309.315 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.502 kb on + strand at 309.503 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 305,963 + -0.2 305,964 - +0.6 305,965 + -0.1 305,966 - -0.6 305,968 - +0.6 305,972 + +1.4 305,978 - +0.3 305,978 - -0.3 305,978 - +0.6 306,211 + GFF3421 0.46 -0.2 306,211 + GFF3421 0.46 +0.4 306,211 + GFF3421 0.46 -0.6 306,212 - GFF3421 0.46 +0.3 306,212 - GFF3421 0.46 +0.3 306,212 - GFF3421 0.46 +0.5 306,212 - GFF3421 0.46 +0.3 306,212 - GFF3421 0.46 -1.5 306,212 - GFF3421 0.46 -0.0 306,212 - GFF3421 0.46 -0.4 306,212 - GFF3421 0.46 +0.0 306,212 - GFF3421 0.46 -0.5 306,872 - +0.4 308,509 + -0.0 308,509 + +0.0 308,509 + -0.3 308,509 + +0.4 308,509 + +0.2 308,509 + -0.8 308,510 - -0.6 308,510 - +0.6 308,510 - +0.4 308,550 - -0.1 308,580 - +0.2 308,580 - +0.1 308,813 + GFF3424 0.32 +1.0 308,813 + GFF3424 0.32 -0.1 308,813 + GFF3424 0.32 +0.4 308,813 + GFF3424 0.32 -0.3 308,814 - GFF3424 0.32 -0.2 308,814 - GFF3424 0.32 +0.5 308,814 - GFF3424 0.32 -0.3 308,814 - GFF3424 0.32 -0.2 308,816 + GFF3424 0.33 -0.0 308,817 - GFF3424 0.33 -0.5 308,817 - GFF3424 0.33 -0.3 308,817 - GFF3424 0.33 -0.3 308,817 - GFF3424 0.33 -1.3 308,817 - GFF3424 0.33 -0.1 308,817 - GFF3424 0.33 -0.2 309,050 + GFF3424 0.60 +0.4 309,050 + GFF3424 0.60 +0.3 309,051 - GFF3424 0.60 +0.6 309,051 - GFF3424 0.60 +0.4 309,101 + GFF3424 0.66 +0.0 309,101 + GFF3424 0.66 +0.0 309,101 + GFF3424 0.66 -0.3 309,101 + GFF3424 0.66 +0.9 309,314 + +0.0 309,314 + +2.1 309,315 - +0.4 309,315 - -0.0 309,435 - -0.6 309,435 - +0.3 309,435 - +0.3 309,435 - -0.2 309,435 - +0.6 309,435 - +0.6 309,435 - +0.0 309,435 - +0.6 309,435 - -0.3 309,435 - +0.8 309,435 - +0.8 309,435 - -0.2 309,502 + -0.4 309,503 - +1.2
Or see this region's nucleotide sequence