Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF182 and GFF183 are separated by 23 nucleotides GFF183 and GFF184 are separated by 4 nucleotides GFF184 and GFF185 overlap by 4 nucleotides GFF185 and GFF186 are separated by 40 nucleotides
GFF182 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 195,040 to 196,035
GFF182
GFF183 - Oligopeptide transport ATP-binding protein OppF (TC 3.A.1.5.1), at 196,059 to 196,973
GFF183
GFF184 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 196,978 to 197,931
GFF184
GFF185 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 197,928 to 198,857
GFF185
GFF186 - hypothetical protein, at 198,898 to 199,665
GFF186
Position (kb) 196
197
198 Strain fitness (log2 ratio) -2
-1
0
1 at 196.031 kb on + strand at 196.031 kb on + strand at 196.031 kb on + strand at 196.031 kb on + strand at 196.031 kb on + strand at 196.032 kb on - strand at 196.032 kb on - strand at 196.972 kb on - strand at 196.972 kb on - strand at 196.987 kb on + strand at 196.988 kb on - strand at 196.988 kb on - strand at 196.988 kb on - strand at 196.988 kb on - strand at 196.994 kb on + strand at 196.995 kb on - strand at 197.047 kb on + strand at 197.047 kb on + strand at 197.048 kb on - strand at 197.048 kb on - strand at 197.572 kb on + strand, within GFF184 at 197.572 kb on + strand, within GFF184 at 197.573 kb on - strand, within GFF184 at 198.148 kb on - strand, within GFF185 at 198.529 kb on - strand, within GFF185
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 196,031 + +0.4 196,031 + -0.3 196,031 + -0.1 196,031 + -0.1 196,031 + -0.0 196,032 - +0.1 196,032 - -1.2 196,972 - -0.3 196,972 - +0.8 196,987 + -0.2 196,988 - +0.0 196,988 - +0.3 196,988 - +0.6 196,988 - +0.8 196,994 + -0.2 196,995 - +0.1 197,047 + -0.3 197,047 + -0.9 197,048 - -0.6 197,048 - -0.7 197,572 + GFF184 0.62 +0.1 197,572 + GFF184 0.62 -0.3 197,573 - GFF184 0.62 -1.8 198,148 - GFF185 0.24 -0.6 198,529 - GFF185 0.65 -0.0
Or see this region's nucleotide sequence