Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1259 and GFF1260 are separated by 126 nucleotides GFF1260 and GFF1261 are separated by 1 nucleotides GFF1261 and GFF1262 are separated by 24 nucleotides
GFF1259 - Glycerol-3-phosphate ABC transporter, periplasmic glycerol-3-phosphate-binding protein (TC 3.A.1.1.3), at 118,027 to 119,346
GFF1259
GFF1260 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 119,473 to 120,354
GFF1260
GFF1261 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 120,356 to 121,207
GFF1261
GFF1262 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 121,232 to 122,236
GFF1262
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 118.480 kb on + strand, within GFF1259 at 118.480 kb on + strand, within GFF1259 at 118.480 kb on + strand, within GFF1259 at 118.481 kb on - strand, within GFF1259 at 118.483 kb on + strand, within GFF1259 at 118.483 kb on + strand, within GFF1259 at 118.777 kb on + strand, within GFF1259 at 118.777 kb on + strand, within GFF1259 at 118.778 kb on - strand, within GFF1259 at 118.778 kb on - strand, within GFF1259 at 118.778 kb on - strand, within GFF1259 at 118.855 kb on + strand, within GFF1259 at 118.855 kb on + strand, within GFF1259 at 118.856 kb on - strand, within GFF1259 at 118.889 kb on - strand, within GFF1259 at 118.889 kb on - strand, within GFF1259 at 118.912 kb on + strand, within GFF1259 at 118.912 kb on + strand, within GFF1259 at 118.913 kb on - strand, within GFF1259 at 118.913 kb on - strand, within GFF1259 at 118.913 kb on - strand, within GFF1259 at 118.996 kb on + strand, within GFF1259 at 118.996 kb on + strand, within GFF1259 at 118.997 kb on - strand, within GFF1259 at 118.997 kb on - strand, within GFF1259 at 119.095 kb on + strand, within GFF1259 at 119.095 kb on + strand, within GFF1259 at 119.123 kb on - strand, within GFF1259 at 119.262 kb on + strand at 119.344 kb on + strand at 119.344 kb on + strand at 119.345 kb on - strand at 119.345 kb on - strand at 119.345 kb on - strand at 119.397 kb on - strand at 119.671 kb on + strand, within GFF1260 at 119.672 kb on - strand, within GFF1260 at 119.789 kb on - strand, within GFF1260 at 119.813 kb on - strand, within GFF1260 at 119.884 kb on + strand, within GFF1260 at 119.885 kb on - strand, within GFF1260 at 119.885 kb on - strand, within GFF1260 at 119.885 kb on - strand, within GFF1260 at 119.885 kb on - strand, within GFF1260 at 119.885 kb on - strand, within GFF1260 at 119.885 kb on - strand, within GFF1260 at 119.885 kb on - strand, within GFF1260 at 119.983 kb on + strand, within GFF1260 at 119.983 kb on + strand, within GFF1260 at 120.143 kb on - strand, within GFF1260 at 120.143 kb on - strand, within GFF1260 at 120.220 kb on + strand, within GFF1260 at 120.229 kb on + strand, within GFF1260 at 120.350 kb on - strand at 120.374 kb on + strand at 120.375 kb on - strand at 120.437 kb on + strand at 120.438 kb on - strand at 120.528 kb on - strand, within GFF1261 at 120.672 kb on - strand, within GFF1261 at 120.758 kb on + strand, within GFF1261 at 120.758 kb on + strand, within GFF1261 at 120.759 kb on - strand, within GFF1261 at 120.759 kb on - strand, within GFF1261 at 120.794 kb on + strand, within GFF1261 at 120.794 kb on + strand, within GFF1261 at 120.794 kb on + strand, within GFF1261 at 120.795 kb on - strand, within GFF1261 at 120.795 kb on - strand, within GFF1261 at 120.795 kb on - strand, within GFF1261 at 121.004 kb on + strand, within GFF1261 at 121.004 kb on + strand, within GFF1261 at 121.004 kb on + strand, within GFF1261 at 121.005 kb on - strand, within GFF1261 at 121.005 kb on - strand, within GFF1261 at 121.005 kb on - strand, within GFF1261 at 121.005 kb on - strand, within GFF1261 at 121.005 kb on - strand, within GFF1261 at 121.044 kb on - strand, within GFF1261 at 121.044 kb on - strand, within GFF1261 at 121.205 kb on + strand at 121.205 kb on + strand at 121.205 kb on + strand at 121.205 kb on + strand at 121.206 kb on - strand at 121.206 kb on - strand at 121.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 118,480 + GFF1259 0.34 -0.0 118,480 + GFF1259 0.34 -2.9 118,480 + GFF1259 0.34 -0.9 118,481 - GFF1259 0.34 -0.1 118,483 + GFF1259 0.35 +0.9 118,483 + GFF1259 0.35 +0.3 118,777 + GFF1259 0.57 -1.5 118,777 + GFF1259 0.57 -0.3 118,778 - GFF1259 0.57 +0.1 118,778 - GFF1259 0.57 -0.0 118,778 - GFF1259 0.57 +0.0 118,855 + GFF1259 0.63 -0.1 118,855 + GFF1259 0.63 +1.7 118,856 - GFF1259 0.63 +0.7 118,889 - GFF1259 0.65 -0.6 118,889 - GFF1259 0.65 +0.6 118,912 + GFF1259 0.67 -0.1 118,912 + GFF1259 0.67 +0.0 118,913 - GFF1259 0.67 -0.0 118,913 - GFF1259 0.67 +0.0 118,913 - GFF1259 0.67 -0.7 118,996 + GFF1259 0.73 +0.6 118,996 + GFF1259 0.73 +0.1 118,997 - GFF1259 0.73 +0.7 118,997 - GFF1259 0.73 -0.6 119,095 + GFF1259 0.81 +0.1 119,095 + GFF1259 0.81 +0.3 119,123 - GFF1259 0.83 +1.5 119,262 + -1.1 119,344 + +0.4 119,344 + -0.9 119,345 - +1.3 119,345 - -0.9 119,345 - +0.4 119,397 - -0.3 119,671 + GFF1260 0.22 -0.3 119,672 - GFF1260 0.23 +0.2 119,789 - GFF1260 0.36 +1.6 119,813 - GFF1260 0.39 +0.8 119,884 + GFF1260 0.47 +0.0 119,885 - GFF1260 0.47 -0.3 119,885 - GFF1260 0.47 -0.3 119,885 - GFF1260 0.47 -0.3 119,885 - GFF1260 0.47 -0.3 119,885 - GFF1260 0.47 +0.8 119,885 - GFF1260 0.47 -0.2 119,885 - GFF1260 0.47 -0.3 119,983 + GFF1260 0.58 -0.1 119,983 + GFF1260 0.58 -0.1 120,143 - GFF1260 0.76 -0.1 120,143 - GFF1260 0.76 -0.2 120,220 + GFF1260 0.85 +0.1 120,229 + GFF1260 0.86 +0.0 120,350 - -0.2 120,374 + -0.7 120,375 - -0.1 120,437 + -1.1 120,438 - -0.1 120,528 - GFF1261 0.20 +0.5 120,672 - GFF1261 0.37 -0.2 120,758 + GFF1261 0.47 -0.1 120,758 + GFF1261 0.47 +0.4 120,759 - GFF1261 0.47 +0.1 120,759 - GFF1261 0.47 -0.2 120,794 + GFF1261 0.51 +1.0 120,794 + GFF1261 0.51 -0.2 120,794 + GFF1261 0.51 -0.3 120,795 - GFF1261 0.52 +0.0 120,795 - GFF1261 0.52 -0.2 120,795 - GFF1261 0.52 +0.1 121,004 + GFF1261 0.76 -0.3 121,004 + GFF1261 0.76 -0.1 121,004 + GFF1261 0.76 +0.5 121,005 - GFF1261 0.76 -0.8 121,005 - GFF1261 0.76 -0.8 121,005 - GFF1261 0.76 -0.2 121,005 - GFF1261 0.76 +0.4 121,005 - GFF1261 0.76 -0.2 121,044 - GFF1261 0.81 +0.4 121,044 - GFF1261 0.81 -0.5 121,205 + +0.1 121,205 + -0.5 121,205 + -0.1 121,205 + -0.1 121,206 - +0.2 121,206 - -0.0 121,269 - -0.2
Or see this region's nucleotide sequence