Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1220 and GFF1221 are separated by 17 nucleotides GFF1221 and GFF1222 overlap by 4 nucleotides GFF1222 and GFF1223 are separated by 19 nucleotides GFF1223 and GFF1224 are separated by 110 nucleotides GFF1224 and GFF1225 are separated by 41 nucleotides
GFF1220 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 84,920 to 85,771
GFF1220
GFF1221 - hypothetical protein, at 85,789 to 86,031
GFF1221
GFF1222 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 86,028 to 87,050
GFF1222
GFF1223 - Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state), at 87,070 to 87,528
GFF1223
GFF1224 - Glutaredoxin 3 (Grx3), at 87,639 to 87,896
GFF1224
GFF1225 - FIG136845: Rhodanese-related sulfurtransferase, at 87,938 to 88,348
GFF1225
Position (kb) 86
87
88 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 86.030 kb on - strand at 86.179 kb on + strand, within GFF1222 at 86.179 kb on + strand, within GFF1222 at 86.179 kb on + strand, within GFF1222 at 86.180 kb on - strand, within GFF1222 at 86.180 kb on - strand, within GFF1222 at 86.503 kb on + strand, within GFF1222 at 86.503 kb on + strand, within GFF1222 at 86.503 kb on + strand, within GFF1222 at 86.503 kb on + strand, within GFF1222 at 86.899 kb on + strand, within GFF1222 at 86.899 kb on + strand, within GFF1222 at 86.899 kb on + strand, within GFF1222 at 87.071 kb on + strand at 87.071 kb on + strand at 87.072 kb on - strand at 87.072 kb on - strand at 87.072 kb on - strand at 87.072 kb on - strand at 87.072 kb on - strand at 87.072 kb on - strand at 87.072 kb on - strand at 87.072 kb on - strand at 87.072 kb on - strand at 87.125 kb on + strand, within GFF1223 at 87.125 kb on + strand, within GFF1223 at 87.125 kb on + strand, within GFF1223 at 87.125 kb on + strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.126 kb on - strand, within GFF1223 at 87.209 kb on + strand, within GFF1223 at 87.209 kb on + strand, within GFF1223 at 87.210 kb on - strand, within GFF1223 at 87.215 kb on + strand, within GFF1223 at 87.215 kb on + strand, within GFF1223 at 87.215 kb on + strand, within GFF1223 at 87.368 kb on + strand, within GFF1223 at 87.368 kb on + strand, within GFF1223 at 87.368 kb on + strand, within GFF1223 at 87.368 kb on + strand, within GFF1223 at 87.368 kb on + strand, within GFF1223 at 87.369 kb on - strand, within GFF1223 at 87.369 kb on - strand, within GFF1223 at 87.369 kb on - strand, within GFF1223 at 87.485 kb on + strand at 87.485 kb on + strand at 87.486 kb on - strand at 87.486 kb on - strand at 87.486 kb on - strand at 87.486 kb on - strand at 87.579 kb on + strand at 87.609 kb on + strand at 87.610 kb on - strand at 87.610 kb on - strand at 87.610 kb on - strand at 87.610 kb on - strand at 87.610 kb on - strand at 87.796 kb on + strand, within GFF1224 at 87.796 kb on + strand, within GFF1224 at 87.796 kb on + strand, within GFF1224 at 87.796 kb on + strand, within GFF1224 at 87.796 kb on + strand, within GFF1224 at 87.796 kb on + strand, within GFF1224 at 87.796 kb on + strand, within GFF1224 at 87.796 kb on + strand, within GFF1224 at 87.796 kb on + strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.797 kb on - strand, within GFF1224 at 87.856 kb on + strand, within GFF1224 at 87.856 kb on + strand, within GFF1224 at 87.856 kb on + strand, within GFF1224 at 87.856 kb on + strand, within GFF1224 at 87.857 kb on - strand, within GFF1224 at 87.857 kb on - strand, within GFF1224 at 87.866 kb on - strand, within GFF1224 at 88.011 kb on + strand, within GFF1225
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 86,030 - +0.7 86,179 + GFF1222 0.15 -3.5 86,179 + GFF1222 0.15 -3.7 86,179 + GFF1222 0.15 -0.9 86,180 - GFF1222 0.15 -4.5 86,180 - GFF1222 0.15 -2.1 86,503 + GFF1222 0.46 -4.5 86,503 + GFF1222 0.46 -3.6 86,503 + GFF1222 0.46 -2.0 86,503 + GFF1222 0.46 -5.9 86,899 + GFF1222 0.85 -1.3 86,899 + GFF1222 0.85 -4.5 86,899 + GFF1222 0.85 -3.4 87,071 + -0.9 87,071 + -1.1 87,072 - +0.5 87,072 - -1.0 87,072 - -1.3 87,072 - +0.1 87,072 - -4.1 87,072 - +0.2 87,072 - -0.2 87,072 - +0.2 87,072 - +1.0 87,125 + GFF1223 0.12 -0.6 87,125 + GFF1223 0.12 -0.3 87,125 + GFF1223 0.12 -0.0 87,125 + GFF1223 0.12 -0.1 87,126 - GFF1223 0.12 +0.2 87,126 - GFF1223 0.12 -1.5 87,126 - GFF1223 0.12 -3.3 87,126 - GFF1223 0.12 -1.0 87,126 - GFF1223 0.12 -3.1 87,126 - GFF1223 0.12 -2.4 87,126 - GFF1223 0.12 +0.4 87,126 - GFF1223 0.12 -1.4 87,126 - GFF1223 0.12 -1.3 87,126 - GFF1223 0.12 -0.1 87,126 - GFF1223 0.12 -0.5 87,126 - GFF1223 0.12 -0.3 87,209 + GFF1223 0.30 -1.0 87,209 + GFF1223 0.30 -1.3 87,210 - GFF1223 0.31 -5.0 87,215 + GFF1223 0.32 -1.9 87,215 + GFF1223 0.32 -0.1 87,215 + GFF1223 0.32 +0.5 87,368 + GFF1223 0.65 -0.7 87,368 + GFF1223 0.65 -0.1 87,368 + GFF1223 0.65 -0.1 87,368 + GFF1223 0.65 -1.0 87,368 + GFF1223 0.65 -0.8 87,369 - GFF1223 0.65 -0.5 87,369 - GFF1223 0.65 -2.2 87,369 - GFF1223 0.65 -0.7 87,485 + -1.0 87,485 + -1.3 87,486 - -1.4 87,486 - -1.1 87,486 - +0.9 87,486 - -1.5 87,579 + -2.4 87,609 + -2.1 87,610 - -0.1 87,610 - +0.3 87,610 - -0.1 87,610 - -0.4 87,610 - +0.1 87,796 + GFF1224 0.61 +0.8 87,796 + GFF1224 0.61 +0.0 87,796 + GFF1224 0.61 +0.4 87,796 + GFF1224 0.61 -0.0 87,796 + GFF1224 0.61 -0.2 87,796 + GFF1224 0.61 +0.4 87,796 + GFF1224 0.61 -0.0 87,796 + GFF1224 0.61 -0.1 87,796 + GFF1224 0.61 -0.0 87,797 - GFF1224 0.61 -0.4 87,797 - GFF1224 0.61 +0.4 87,797 - GFF1224 0.61 +0.7 87,797 - GFF1224 0.61 +0.2 87,797 - GFF1224 0.61 +1.1 87,797 - GFF1224 0.61 +0.1 87,797 - GFF1224 0.61 +0.4 87,797 - GFF1224 0.61 +0.4 87,797 - GFF1224 0.61 +0.0 87,797 - GFF1224 0.61 +0.1 87,856 + GFF1224 0.84 +0.2 87,856 + GFF1224 0.84 -0.1 87,856 + GFF1224 0.84 +0.2 87,856 + GFF1224 0.84 +0.5 87,857 - GFF1224 0.84 -0.1 87,857 - GFF1224 0.84 -0.6 87,866 - GFF1224 0.88 -0.1 88,011 + GFF1225 0.18 +1.3
Or see this region's nucleotide sequence