Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.3 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS14475 and COO64_RS14480 are separated by 30 nucleotides COO64_RS14480 and COO64_RS14485 are separated by 144 nucleotides COO64_RS14485 and COO64_RS14490 are separated by 84 nucleotides
COO64_RS14475: COO64_RS14475 - DMT family transporter, at 457,532 to 458,476
_RS14475
COO64_RS14480: COO64_RS14480 - hypothetical protein, at 458,507 to 458,893
_RS14480
COO64_RS14485: COO64_RS14485 - MOSC domain-containing protein, at 459,038 to 459,742
_RS14485
COO64_RS14490: COO64_RS14490 - gamma-glutamyl-gamma-aminobutyrate hydrolase family protein, at 459,827 to 460,642
_RS14490
Position (kb) 459
460 Strain fitness (log2 ratio) -2
-1
0
1
2 at 458.155 kb on - strand, within COO64_RS14475 at 458.234 kb on + strand, within COO64_RS14475 at 458.234 kb on + strand, within COO64_RS14475 at 458.471 kb on - strand at 458.471 kb on - strand at 458.471 kb on - strand at 458.474 kb on + strand at 458.474 kb on + strand at 458.475 kb on - strand at 458.475 kb on - strand at 458.475 kb on - strand at 458.475 kb on - strand at 458.715 kb on + strand, within COO64_RS14480 at 458.715 kb on + strand, within COO64_RS14480 at 458.716 kb on - strand, within COO64_RS14480 at 458.716 kb on - strand, within COO64_RS14480 at 458.716 kb on - strand, within COO64_RS14480 at 458.716 kb on - strand, within COO64_RS14480 at 458.959 kb on + strand at 458.959 kb on + strand at 458.959 kb on + strand at 458.959 kb on + strand at 458.959 kb on + strand at 458.959 kb on + strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.963 kb on + strand at 459.021 kb on - strand at 459.021 kb on - strand at 459.069 kb on + strand at 459.069 kb on + strand at 459.070 kb on - strand at 459.070 kb on - strand at 459.070 kb on - strand at 459.070 kb on - strand at 459.070 kb on - strand at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.345 kb on + strand, within COO64_RS14485 at 459.345 kb on + strand, within COO64_RS14485 at 459.529 kb on + strand, within COO64_RS14485 at 459.758 kb on + strand at 459.758 kb on + strand at 459.759 kb on - strand at 459.759 kb on - strand at 459.759 kb on - strand at 459.792 kb on + strand at 459.792 kb on + strand at 459.793 kb on - strand at 459.793 kb on - strand at 460.155 kb on + strand, within COO64_RS14490 at 460.156 kb on - strand, within COO64_RS14490 at 460.156 kb on - strand, within COO64_RS14490 at 460.156 kb on - strand, within COO64_RS14490 at 460.156 kb on - strand, within COO64_RS14490 at 460.216 kb on - strand, within COO64_RS14490 at 460.228 kb on - strand, within COO64_RS14490 at 460.313 kb on + strand, within COO64_RS14490 at 460.313 kb on + strand, within COO64_RS14490 at 460.446 kb on + strand, within COO64_RS14490 at 460.493 kb on + strand, within COO64_RS14490 at 460.493 kb on + strand, within COO64_RS14490 at 460.494 kb on - strand, within COO64_RS14490 at 460.494 kb on - strand, within COO64_RS14490 at 460.494 kb on - strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.528 kb on - strand, within COO64_RS14490 at 460.528 kb on - strand, within COO64_RS14490 at 460.528 kb on - strand, within COO64_RS14490 at 460.549 kb on - strand, within COO64_RS14490 at 460.618 kb on + strand at 460.619 kb on - strand at 460.619 kb on - strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.674 kb on + strand at 460.675 kb on - strand at 460.675 kb on - strand at 460.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.3 X remove 458,155 - COO64_RS14475 0.66 +0.3 458,234 + COO64_RS14475 0.74 +1.3 458,234 + COO64_RS14475 0.74 +0.1 458,471 - -0.5 458,471 - -0.2 458,471 - -0.2 458,474 + -0.4 458,474 + -1.3 458,475 - -0.4 458,475 - -0.2 458,475 - -1.8 458,475 - +0.5 458,715 + COO64_RS14480 0.54 -0.2 458,715 + COO64_RS14480 0.54 -1.9 458,716 - COO64_RS14480 0.54 -0.6 458,716 - COO64_RS14480 0.54 -1.9 458,716 - COO64_RS14480 0.54 +0.4 458,716 - COO64_RS14480 0.54 -0.4 458,959 + +0.2 458,959 + +0.5 458,959 + -0.0 458,959 + -0.2 458,959 + +0.5 458,959 + +0.1 458,960 - -0.3 458,960 - +0.4 458,960 - +0.2 458,960 - +0.3 458,960 - +0.3 458,960 - -1.5 458,960 - +1.3 458,963 + +0.4 459,021 - -0.6 459,021 - +0.6 459,069 + -0.2 459,069 + +1.1 459,070 - -0.6 459,070 - +0.3 459,070 - -0.3 459,070 - +0.4 459,070 - -0.2 459,245 + COO64_RS14485 0.29 -0.6 459,245 + COO64_RS14485 0.29 +1.5 459,245 + COO64_RS14485 0.29 -0.6 459,245 + COO64_RS14485 0.29 +0.3 459,245 + COO64_RS14485 0.29 +0.4 459,245 + COO64_RS14485 0.29 +0.7 459,245 + COO64_RS14485 0.29 +0.3 459,245 + COO64_RS14485 0.29 +0.4 459,245 + COO64_RS14485 0.29 +0.3 459,246 - COO64_RS14485 0.30 +0.5 459,246 - COO64_RS14485 0.30 +0.1 459,246 - COO64_RS14485 0.30 -0.6 459,246 - COO64_RS14485 0.30 -0.4 459,246 - COO64_RS14485 0.30 -0.4 459,345 + COO64_RS14485 0.44 -0.1 459,345 + COO64_RS14485 0.44 +0.3 459,529 + COO64_RS14485 0.70 +0.0 459,758 + +0.4 459,758 + +0.3 459,759 - +0.2 459,759 - +0.6 459,759 - +0.0 459,792 + -1.0 459,792 + +1.1 459,793 - -0.1 459,793 - -0.3 460,155 + COO64_RS14490 0.40 +0.6 460,156 - COO64_RS14490 0.40 +0.1 460,156 - COO64_RS14490 0.40 +0.2 460,156 - COO64_RS14490 0.40 +1.3 460,156 - COO64_RS14490 0.40 +0.4 460,216 - COO64_RS14490 0.48 -0.2 460,228 - COO64_RS14490 0.49 +0.5 460,313 + COO64_RS14490 0.60 -0.6 460,313 + COO64_RS14490 0.60 +0.5 460,446 + COO64_RS14490 0.76 -0.2 460,493 + COO64_RS14490 0.82 -0.2 460,493 + COO64_RS14490 0.82 -2.2 460,494 - COO64_RS14490 0.82 +0.0 460,494 - COO64_RS14490 0.82 +0.1 460,494 - COO64_RS14490 0.82 +0.4 460,527 + COO64_RS14490 0.86 +1.8 460,527 + COO64_RS14490 0.86 -0.2 460,527 + COO64_RS14490 0.86 -0.9 460,527 + COO64_RS14490 0.86 +0.2 460,527 + COO64_RS14490 0.86 -0.2 460,527 + COO64_RS14490 0.86 +0.3 460,528 - COO64_RS14490 0.86 -0.2 460,528 - COO64_RS14490 0.86 +1.6 460,528 - COO64_RS14490 0.86 +0.2 460,549 - COO64_RS14490 0.88 -0.3 460,618 + -0.6 460,619 - -0.0 460,619 - -0.0 460,642 + -0.2 460,642 + +0.0 460,642 + +1.2 460,642 + -0.4 460,642 + -0.5 460,642 + +0.6 460,642 + +0.6 460,642 + +0.3 460,642 + +0.4 460,642 + -0.3 460,643 - +0.2 460,643 - +0.3 460,643 - -0.5 460,643 - -0.2 460,643 - -0.2 460,643 - +0.6 460,674 + +0.5 460,675 - +0.5 460,675 - +0.9 460,675 - +1.1
Or see this region's nucleotide sequence