Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF875 and GFF876 are separated by 45 nucleotides GFF876 and GFF877 are separated by 78 nucleotides
GFF875 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 599,290 to 601,422
GFF875
GFF876 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 601,468 to 602,676
GFF876
GFF877 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 602,755 to 603,891
GFF877
Position (kb) 601
602
603 Strain fitness (log2 ratio) -2
-1
0
1 at 600.488 kb on + strand, within GFF875 at 600.488 kb on + strand, within GFF875 at 601.089 kb on - strand, within GFF875 at 601.409 kb on + strand at 601.409 kb on + strand at 601.409 kb on + strand at 601.709 kb on + strand, within GFF876 at 601.986 kb on - strand, within GFF876 at 602.171 kb on + strand, within GFF876 at 602.171 kb on + strand, within GFF876 at 602.171 kb on + strand, within GFF876 at 602.202 kb on - strand, within GFF876 at 602.246 kb on + strand, within GFF876 at 602.246 kb on + strand, within GFF876 at 602.246 kb on + strand, within GFF876 at 602.561 kb on - strand at 602.756 kb on + strand at 602.757 kb on - strand at 602.757 kb on - strand at 602.927 kb on + strand, within GFF877 at 602.928 kb on - strand, within GFF877 at 602.928 kb on - strand, within GFF877 at 603.600 kb on - strand, within GFF877 at 603.600 kb on - strand, within GFF877 at 603.600 kb on - strand, within GFF877
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 600,488 + GFF875 0.56 -0.2 600,488 + GFF875 0.56 -1.0 601,089 - GFF875 0.84 -0.5 601,409 + -0.1 601,409 + -0.8 601,409 + +0.2 601,709 + GFF876 0.20 +0.7 601,986 - GFF876 0.43 +0.1 602,171 + GFF876 0.58 +0.0 602,171 + GFF876 0.58 -0.5 602,171 + GFF876 0.58 -0.5 602,202 - GFF876 0.61 +0.8 602,246 + GFF876 0.64 -0.1 602,246 + GFF876 0.64 -0.9 602,246 + GFF876 0.64 +0.4 602,561 - +0.1 602,756 + +0.2 602,757 - -0.4 602,757 - +0.4 602,927 + GFF877 0.15 +0.4 602,928 - GFF877 0.15 -0.3 602,928 - GFF877 0.15 -0.1 603,600 - GFF877 0.74 -0.4 603,600 - GFF877 0.74 -1.8 603,600 - GFF877 0.74 -2.6
Or see this region's nucleotide sequence