Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5253 and GFF5254 overlap by 4 nucleotides GFF5254 and GFF5255 are separated by 19 nucleotides GFF5255 and GFF5256 overlap by 4 nucleotides GFF5256 and GFF5257 are separated by 13 nucleotides
GFF5253 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,268,923 to 2,270,455
GFF5253
GFF5254 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 2,270,452 to 2,271,390
GFF5254
GFF5255 - ABC transporter substrate-binding protein, at 2,271,410 to 2,272,357
GFF5255
GFF5256 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 2,272,354 to 2,273,133
GFF5256
GFF5257 - Alkanesulfonates transport system permease protein, at 2,273,147 to 2,273,911
GFF5257
Position (kb)
2271
2272
2273 Strain fitness (log2 ratio)
-1
0
1 at 2270.827 kb on + strand, within GFF5254 at 2270.828 kb on - strand, within GFF5254 at 2270.887 kb on + strand, within GFF5254 at 2270.887 kb on + strand, within GFF5254 at 2271.131 kb on - strand, within GFF5254 at 2271.389 kb on + strand at 2271.389 kb on - strand at 2271.741 kb on - strand, within GFF5255 at 2271.741 kb on - strand, within GFF5255 at 2271.741 kb on - strand, within GFF5255 at 2271.741 kb on - strand, within GFF5255 at 2271.896 kb on + strand, within GFF5255 at 2272.034 kb on + strand, within GFF5255 at 2272.034 kb on + strand, within GFF5255 at 2272.038 kb on - strand, within GFF5255 at 2272.202 kb on + strand, within GFF5255 at 2272.202 kb on + strand, within GFF5255 at 2272.203 kb on - strand, within GFF5255 at 2272.349 kb on + strand at 2272.349 kb on + strand at 2272.350 kb on - strand at 2272.350 kb on - strand at 2272.350 kb on - strand at 2272.562 kb on - strand, within GFF5256 at 2272.929 kb on - strand, within GFF5256 at 2272.969 kb on + strand, within GFF5256
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 2,270,827 + GFF5254 0.40 -0.5 2,270,828 - GFF5254 0.40 +0.0 2,270,887 + GFF5254 0.46 -0.4 2,270,887 + GFF5254 0.46 +0.6 2,271,131 - GFF5254 0.72 +0.1 2,271,389 + +0.0 2,271,389 - +0.4 2,271,741 - GFF5255 0.35 -0.2 2,271,741 - GFF5255 0.35 +0.7 2,271,741 - GFF5255 0.35 -0.7 2,271,741 - GFF5255 0.35 -0.1 2,271,896 + GFF5255 0.51 -0.6 2,272,034 + GFF5255 0.66 +0.4 2,272,034 + GFF5255 0.66 -0.5 2,272,038 - GFF5255 0.66 -0.3 2,272,202 + GFF5255 0.84 +0.1 2,272,202 + GFF5255 0.84 -0.6 2,272,203 - GFF5255 0.84 -0.4 2,272,349 + +1.0 2,272,349 + +0.2 2,272,350 - -0.2 2,272,350 - +0.8 2,272,350 - +0.2 2,272,562 - GFF5256 0.27 -0.1 2,272,929 - GFF5256 0.74 +0.2 2,272,969 + GFF5256 0.79 -0.0
Or see this region's nucleotide sequence