Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF515 and GFF516 are separated by 7 nucleotides GFF516 and GFF517 are separated by 38 nucleotides GFF517 and GFF518 are separated by 67 nucleotides
GFF515 - Glutamine amidotransferase class-I (EC 6.3.5.2), at 182,782 to 183,522
GFF515
GFF516 - Acyl-CoA hydrolase (EC 3.1.2.20), at 183,530 to 183,970
GFF516
GFF517 - Lipid A export ATP-binding/permease protein MsbA, at 184,009 to 185,856
GFF517
GFF518 - Transcriptional regulator, at 185,924 to 186,841
GFF518
Position (kb) 184
185
186 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 183.205 kb on + strand, within GFF515 at 183.205 kb on + strand, within GFF515 at 183.206 kb on - strand, within GFF515 at 183.380 kb on - strand, within GFF515 at 183.380 kb on - strand, within GFF515 at 183.487 kb on + strand at 183.487 kb on + strand at 183.487 kb on + strand at 183.487 kb on + strand at 183.487 kb on + strand at 183.607 kb on - strand, within GFF516 at 183.607 kb on - strand, within GFF516 at 183.607 kb on - strand, within GFF516 at 183.636 kb on + strand, within GFF516 at 183.636 kb on + strand, within GFF516 at 183.804 kb on + strand, within GFF516 at 183.804 kb on + strand, within GFF516 at 184.124 kb on - strand at 184.135 kb on + strand at 184.135 kb on + strand at 184.136 kb on - strand at 184.504 kb on + strand, within GFF517 at 184.505 kb on - strand, within GFF517 at 184.514 kb on - strand, within GFF517 at 184.586 kb on - strand, within GFF517 at 184.618 kb on + strand, within GFF517 at 184.618 kb on + strand, within GFF517 at 184.618 kb on + strand, within GFF517 at 184.619 kb on - strand, within GFF517 at 184.619 kb on - strand, within GFF517 at 184.619 kb on - strand, within GFF517 at 184.726 kb on + strand, within GFF517 at 184.726 kb on + strand, within GFF517 at 184.727 kb on - strand, within GFF517 at 184.727 kb on - strand, within GFF517 at 184.727 kb on - strand, within GFF517 at 184.727 kb on - strand, within GFF517 at 184.727 kb on - strand, within GFF517 at 184.741 kb on + strand, within GFF517 at 184.741 kb on + strand, within GFF517 at 184.741 kb on + strand, within GFF517 at 184.742 kb on - strand, within GFF517 at 184.742 kb on - strand, within GFF517 at 184.742 kb on - strand, within GFF517 at 184.969 kb on + strand, within GFF517 at 184.969 kb on + strand, within GFF517 at 185.146 kb on + strand, within GFF517 at 185.260 kb on + strand, within GFF517 at 185.261 kb on - strand, within GFF517 at 185.262 kb on - strand, within GFF517 at 185.269 kb on + strand, within GFF517 at 185.269 kb on + strand, within GFF517 at 185.269 kb on + strand, within GFF517 at 185.269 kb on + strand, within GFF517 at 185.270 kb on - strand, within GFF517 at 185.270 kb on - strand, within GFF517 at 185.270 kb on - strand, within GFF517 at 185.407 kb on + strand, within GFF517 at 185.408 kb on - strand, within GFF517 at 185.419 kb on + strand, within GFF517 at 185.510 kb on - strand, within GFF517 at 185.510 kb on - strand, within GFF517 at 185.567 kb on + strand, within GFF517 at 185.815 kb on + strand at 185.815 kb on + strand at 185.815 kb on + strand at 185.815 kb on + strand at 185.815 kb on + strand at 185.816 kb on - strand at 185.912 kb on + strand at 186.116 kb on + strand, within GFF518 at 186.443 kb on + strand, within GFF518 at 186.443 kb on + strand, within GFF518 at 186.443 kb on + strand, within GFF518 at 186.444 kb on - strand, within GFF518 at 186.444 kb on - strand, within GFF518 at 186.522 kb on - strand, within GFF518 at 186.536 kb on + strand, within GFF518 at 186.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 183,205 + GFF515 0.57 -0.3 183,205 + GFF515 0.57 +0.1 183,206 - GFF515 0.57 +0.1 183,380 - GFF515 0.81 +0.5 183,380 - GFF515 0.81 +0.2 183,487 + +0.1 183,487 + -0.3 183,487 + +0.5 183,487 + +0.8 183,487 + +0.9 183,607 - GFF516 0.17 -0.5 183,607 - GFF516 0.17 +0.1 183,607 - GFF516 0.17 +0.1 183,636 + GFF516 0.24 -0.2 183,636 + GFF516 0.24 +0.3 183,804 + GFF516 0.62 +2.0 183,804 + GFF516 0.62 -0.7 184,124 - +0.1 184,135 + +0.5 184,135 + +1.7 184,136 - -0.1 184,504 + GFF517 0.27 +0.2 184,505 - GFF517 0.27 +0.7 184,514 - GFF517 0.27 +0.1 184,586 - GFF517 0.31 -0.1 184,618 + GFF517 0.33 +1.0 184,618 + GFF517 0.33 -0.8 184,618 + GFF517 0.33 +0.3 184,619 - GFF517 0.33 +0.3 184,619 - GFF517 0.33 +0.0 184,619 - GFF517 0.33 -0.1 184,726 + GFF517 0.39 +0.2 184,726 + GFF517 0.39 -0.2 184,727 - GFF517 0.39 -0.2 184,727 - GFF517 0.39 -0.1 184,727 - GFF517 0.39 +0.3 184,727 - GFF517 0.39 +0.2 184,727 - GFF517 0.39 -0.1 184,741 + GFF517 0.40 +0.1 184,741 + GFF517 0.40 -0.2 184,741 + GFF517 0.40 +0.8 184,742 - GFF517 0.40 -0.8 184,742 - GFF517 0.40 +0.4 184,742 - GFF517 0.40 +1.4 184,969 + GFF517 0.52 -1.9 184,969 + GFF517 0.52 -0.1 185,146 + GFF517 0.62 +0.6 185,260 + GFF517 0.68 +1.0 185,261 - GFF517 0.68 +0.1 185,262 - GFF517 0.68 +0.3 185,269 + GFF517 0.68 -0.1 185,269 + GFF517 0.68 +0.4 185,269 + GFF517 0.68 -0.6 185,269 + GFF517 0.68 +0.2 185,270 - GFF517 0.68 +1.0 185,270 - GFF517 0.68 -1.4 185,270 - GFF517 0.68 -0.2 185,407 + GFF517 0.76 +0.3 185,408 - GFF517 0.76 -0.1 185,419 + GFF517 0.76 +0.1 185,510 - GFF517 0.81 +1.0 185,510 - GFF517 0.81 +0.2 185,567 + GFF517 0.84 -0.2 185,815 + +2.2 185,815 + -0.6 185,815 + +0.2 185,815 + +1.2 185,815 + -0.3 185,816 - +0.2 185,912 + +0.1 186,116 + GFF518 0.21 +4.2 186,443 + GFF518 0.57 +3.6 186,443 + GFF518 0.57 +3.8 186,443 + GFF518 0.57 +3.6 186,444 - GFF518 0.57 +2.6 186,444 - GFF518 0.57 +3.7 186,522 - GFF518 0.65 +3.2 186,536 + GFF518 0.67 +4.4 186,855 + +0.3
Or see this region's nucleotide sequence