Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4720 and GFF4721 are separated by 80 nucleotides GFF4721 and GFF4722 are separated by 100 nucleotides GFF4722 and GFF4723 are separated by 4 nucleotides
GFF4720 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,672,443 to 1,673,411
GFF4720
GFF4721 - Mhp operon transcriptional activator, at 1,673,492 to 1,674,310
GFF4721
GFF4722 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,674,411 to 1,675,175
GFF4722
GFF4723 - Negative transcriptional regulator, at 1,675,180 to 1,675,797
GFF4723
Position (kb)
1673
1674
1675 Strain fitness (log2 ratio)
-1
0
1
2 at 1672.861 kb on - strand, within GFF4720 at 1673.313 kb on + strand, within GFF4720 at 1673.314 kb on - strand, within GFF4720 at 1673.314 kb on - strand, within GFF4720 at 1673.314 kb on - strand, within GFF4720 at 1673.407 kb on - strand at 1673.407 kb on - strand at 1673.440 kb on + strand at 1673.441 kb on - strand at 1673.678 kb on + strand, within GFF4721 at 1673.678 kb on + strand, within GFF4721 at 1673.678 kb on + strand, within GFF4721 at 1673.678 kb on + strand, within GFF4721 at 1673.679 kb on - strand, within GFF4721 at 1673.879 kb on + strand, within GFF4721 at 1673.880 kb on - strand, within GFF4721 at 1673.955 kb on - strand, within GFF4721 at 1674.263 kb on + strand at 1674.263 kb on + strand at 1674.264 kb on - strand at 1674.264 kb on - strand at 1675.247 kb on + strand, within GFF4723
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 1,672,861 - GFF4720 0.43 -0.5 1,673,313 + GFF4720 0.90 -0.1 1,673,314 - GFF4720 0.90 -0.2 1,673,314 - GFF4720 0.90 +1.3 1,673,314 - GFF4720 0.90 +0.4 1,673,407 - -0.1 1,673,407 - +1.8 1,673,440 + -0.0 1,673,441 - -0.6 1,673,678 + GFF4721 0.23 -0.1 1,673,678 + GFF4721 0.23 -0.5 1,673,678 + GFF4721 0.23 -0.3 1,673,678 + GFF4721 0.23 +0.1 1,673,679 - GFF4721 0.23 +0.8 1,673,879 + GFF4721 0.47 -0.4 1,673,880 - GFF4721 0.47 +0.6 1,673,955 - GFF4721 0.57 -0.7 1,674,263 + -0.4 1,674,263 + +0.9 1,674,264 - -0.2 1,674,264 - -0.3 1,675,247 + GFF4723 0.11 +1.2
Or see this region's nucleotide sequence