Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4631 and GFF4632 are separated by 1 nucleotides GFF4632 and GFF4633 overlap by 4 nucleotides
GFF4631 - Putative 2Fe-2S ferredoxin CbiW involved in B12 biosynthesis, at 1,583,609 to 1,583,959
GFF4631
GFF4632 - 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I, at 1,583,961 to 1,584,347
GFF4632
GFF4633 - Queuosine Biosynthesis QueE Radical SAM, at 1,584,344 to 1,584,979
GFF4633
Position (kb)
1583
1584 Strain fitness (log2 ratio)
-2
-1
0
1 at 1582.663 kb on - strand at 1582.663 kb on - strand at 1582.663 kb on - strand at 1582.663 kb on - strand at 1582.712 kb on - strand at 1582.715 kb on + strand at 1582.715 kb on + strand at 1582.715 kb on + strand at 1582.715 kb on + strand at 1582.715 kb on + strand at 1582.716 kb on - strand at 1582.716 kb on - strand at 1582.716 kb on - strand at 1582.716 kb on - strand at 1582.716 kb on - strand at 1582.716 kb on - strand at 1582.734 kb on + strand at 1582.734 kb on + strand at 1582.748 kb on + strand at 1582.749 kb on - strand at 1582.833 kb on - strand at 1582.846 kb on + strand at 1582.893 kb on + strand at 1582.893 kb on + strand at 1582.933 kb on + strand at 1582.935 kb on + strand at 1582.940 kb on + strand at 1582.940 kb on + strand at 1582.940 kb on + strand at 1582.940 kb on + strand at 1582.940 kb on + strand at 1582.940 kb on + strand at 1582.941 kb on - strand at 1583.015 kb on - strand at 1583.078 kb on - strand at 1583.094 kb on + strand at 1583.094 kb on + strand at 1583.094 kb on + strand at 1583.095 kb on - strand at 1583.095 kb on - strand at 1583.096 kb on + strand at 1583.096 kb on + strand at 1583.096 kb on + strand at 1583.096 kb on + strand at 1583.096 kb on + strand at 1583.097 kb on - strand at 1583.250 kb on + strand at 1583.250 kb on + strand at 1583.250 kb on + strand at 1583.251 kb on - strand at 1583.251 kb on - strand at 1583.326 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.328 kb on - strand at 1583.501 kb on + strand at 1583.501 kb on + strand at 1583.501 kb on + strand at 1583.501 kb on + strand at 1583.502 kb on - strand at 1583.502 kb on - strand at 1583.509 kb on + strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.510 kb on - strand at 1583.721 kb on + strand, within GFF4631 at 1583.721 kb on + strand, within GFF4631 at 1583.721 kb on + strand, within GFF4631 at 1583.721 kb on + strand, within GFF4631 at 1583.721 kb on + strand, within GFF4631 at 1583.722 kb on - strand, within GFF4631 at 1583.722 kb on - strand, within GFF4631 at 1583.722 kb on - strand, within GFF4631 at 1583.724 kb on + strand, within GFF4631 at 1583.725 kb on - strand, within GFF4631 at 1583.854 kb on + strand, within GFF4631 at 1583.888 kb on - strand, within GFF4631 at 1583.888 kb on - strand, within GFF4631 at 1583.959 kb on + strand at 1583.960 kb on - strand at 1584.374 kb on + strand at 1584.374 kb on + strand at 1584.374 kb on + strand at 1584.375 kb on - strand at 1584.375 kb on - strand at 1584.375 kb on - strand at 1584.409 kb on - strand, within GFF4633 at 1584.409 kb on - strand, within GFF4633 at 1584.581 kb on - strand, within GFF4633 at 1584.753 kb on - strand, within GFF4633
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 1,582,663 - -0.0 1,582,663 - -0.3 1,582,663 - -0.3 1,582,663 - -0.1 1,582,712 - -0.4 1,582,715 + +0.2 1,582,715 + +0.1 1,582,715 + +0.8 1,582,715 + +0.6 1,582,715 + +0.6 1,582,716 - -1.1 1,582,716 - +0.2 1,582,716 - -1.7 1,582,716 - +0.4 1,582,716 - +0.1 1,582,716 - +1.3 1,582,734 + -0.2 1,582,734 + -0.0 1,582,748 + -0.1 1,582,749 - +0.1 1,582,833 - -0.1 1,582,846 + +0.5 1,582,893 + +0.1 1,582,893 + +1.1 1,582,933 + +0.2 1,582,935 + +0.1 1,582,940 + -0.3 1,582,940 + +0.0 1,582,940 + +0.6 1,582,940 + +0.5 1,582,940 + -0.1 1,582,940 + -1.2 1,582,941 - +0.7 1,583,015 - +0.2 1,583,078 - +0.1 1,583,094 + -0.3 1,583,094 + +1.2 1,583,094 + -0.6 1,583,095 - +1.1 1,583,095 - -0.3 1,583,096 + +0.3 1,583,096 + -0.3 1,583,096 + +0.6 1,583,096 + -0.6 1,583,096 + +0.3 1,583,097 - -0.5 1,583,250 + +0.6 1,583,250 + -0.2 1,583,250 + -0.4 1,583,251 - -1.0 1,583,251 - +1.6 1,583,326 + -0.9 1,583,327 + +0.4 1,583,327 + -0.4 1,583,327 + -0.3 1,583,327 + +0.3 1,583,327 + +0.0 1,583,327 + +0.2 1,583,327 + -1.3 1,583,327 + -1.0 1,583,327 + -0.1 1,583,327 + -0.8 1,583,327 + +0.0 1,583,327 + -0.5 1,583,327 + -0.7 1,583,328 - -0.2 1,583,328 - -0.5 1,583,328 - -0.4 1,583,328 - -0.3 1,583,328 - -0.0 1,583,328 - +0.6 1,583,328 - -0.2 1,583,328 - -0.9 1,583,328 - -2.3 1,583,328 - +1.0 1,583,501 + +0.5 1,583,501 + -0.1 1,583,501 + -1.0 1,583,501 + +0.4 1,583,502 - +0.7 1,583,502 - -0.4 1,583,509 + +0.9 1,583,510 - +0.1 1,583,510 - -0.1 1,583,510 - +1.1 1,583,510 - +1.2 1,583,510 - -0.4 1,583,510 - +0.4 1,583,510 - -0.9 1,583,510 - -0.1 1,583,510 - -0.3 1,583,510 - +0.6 1,583,721 + GFF4631 0.32 -0.9 1,583,721 + GFF4631 0.32 +1.3 1,583,721 + GFF4631 0.32 -0.3 1,583,721 + GFF4631 0.32 -0.5 1,583,721 + GFF4631 0.32 +0.4 1,583,722 - GFF4631 0.32 +0.1 1,583,722 - GFF4631 0.32 -0.2 1,583,722 - GFF4631 0.32 +0.1 1,583,724 + GFF4631 0.33 -1.4 1,583,725 - GFF4631 0.33 +0.3 1,583,854 + GFF4631 0.70 -0.0 1,583,888 - GFF4631 0.79 -0.2 1,583,888 - GFF4631 0.79 -0.4 1,583,959 + -0.3 1,583,960 - +0.3 1,584,374 + +0.1 1,584,374 + -0.5 1,584,374 + -0.1 1,584,375 - -0.1 1,584,375 - -0.4 1,584,375 - +0.3 1,584,409 - GFF4633 0.10 -0.4 1,584,409 - GFF4633 0.10 -0.1 1,584,581 - GFF4633 0.37 -0.6 1,584,753 - GFF4633 0.64 -0.4
Or see this region's nucleotide sequence