Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3092 and GFF3093 are separated by 22 nucleotides GFF3093 and GFF3094 overlap by 8 nucleotides GFF3094 and GFF3095 are separated by 41 nucleotides GFF3095 and GFF3096 are separated by 75 nucleotides
GFF3092 - Phosphatidylglycerophosphatase A (EC 3.1.3.27), at 169,466 to 169,984
GFF3092
GFF3093 - Thiamine-monophosphate kinase (EC 2.7.4.16), at 170,007 to 170,978
GFF3093
GFF3094 - Site-specific recombinase, at 170,971 to 173,013
GFF3094
GFF3095 - ApaG protein, at 173,055 to 173,459
GFF3095
GFF3096 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 173,535 to 174,218
GFF3096
Position (kb) 170
171
172
173
174 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 169.993 kb on + strand at 171.167 kb on + strand at 171.167 kb on + strand at 171.167 kb on + strand at 171.167 kb on + strand at 171.168 kb on - strand at 171.329 kb on + strand, within GFF3094 at 171.500 kb on + strand, within GFF3094 at 171.501 kb on - strand, within GFF3094 at 171.882 kb on - strand, within GFF3094 at 171.882 kb on - strand, within GFF3094 at 171.882 kb on - strand, within GFF3094 at 172.031 kb on + strand, within GFF3094 at 172.031 kb on + strand, within GFF3094 at 172.032 kb on - strand, within GFF3094 at 172.032 kb on - strand, within GFF3094 at 172.032 kb on - strand, within GFF3094 at 172.032 kb on - strand, within GFF3094 at 172.032 kb on - strand, within GFF3094 at 172.032 kb on - strand, within GFF3094 at 172.286 kb on + strand, within GFF3094 at 172.287 kb on - strand, within GFF3094 at 172.287 kb on - strand, within GFF3094 at 172.296 kb on - strand, within GFF3094 at 172.509 kb on - strand, within GFF3094 at 172.739 kb on + strand, within GFF3094 at 173.209 kb on + strand, within GFF3095 at 173.209 kb on + strand, within GFF3095 at 173.209 kb on + strand, within GFF3095 at 173.209 kb on + strand, within GFF3095 at 173.209 kb on + strand, within GFF3095 at 173.209 kb on + strand, within GFF3095 at 173.210 kb on - strand, within GFF3095 at 173.210 kb on - strand, within GFF3095 at 173.210 kb on - strand, within GFF3095 at 173.362 kb on + strand, within GFF3095 at 173.363 kb on - strand, within GFF3095 at 173.363 kb on - strand, within GFF3095 at 173.374 kb on + strand, within GFF3095 at 173.374 kb on + strand, within GFF3095 at 173.374 kb on + strand, within GFF3095 at 173.375 kb on - strand, within GFF3095 at 173.375 kb on - strand, within GFF3095 at 173.375 kb on - strand, within GFF3095 at 173.375 kb on - strand, within GFF3095 at 173.375 kb on - strand, within GFF3095 at 173.440 kb on + strand at 173.440 kb on + strand at 173.440 kb on + strand at 173.440 kb on + strand at 173.440 kb on + strand at 173.441 kb on - strand at 173.441 kb on - strand at 173.441 kb on - strand at 173.462 kb on + strand at 173.462 kb on + strand at 173.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 169,993 + -2.0 171,167 + +0.3 171,167 + -0.9 171,167 + -0.0 171,167 + +0.3 171,168 - +0.1 171,329 + GFF3094 0.18 -0.8 171,500 + GFF3094 0.26 +0.2 171,501 - GFF3094 0.26 +0.1 171,882 - GFF3094 0.45 -0.0 171,882 - GFF3094 0.45 -0.1 171,882 - GFF3094 0.45 -0.1 172,031 + GFF3094 0.52 +0.0 172,031 + GFF3094 0.52 -0.3 172,032 - GFF3094 0.52 -0.8 172,032 - GFF3094 0.52 -1.3 172,032 - GFF3094 0.52 +0.1 172,032 - GFF3094 0.52 -2.0 172,032 - GFF3094 0.52 +0.3 172,032 - GFF3094 0.52 -0.1 172,286 + GFF3094 0.64 +0.2 172,287 - GFF3094 0.64 -3.0 172,287 - GFF3094 0.64 -0.1 172,296 - GFF3094 0.65 +0.4 172,509 - GFF3094 0.75 -0.5 172,739 + GFF3094 0.87 -1.2 173,209 + GFF3095 0.38 +0.0 173,209 + GFF3095 0.38 -1.1 173,209 + GFF3095 0.38 -0.2 173,209 + GFF3095 0.38 +1.0 173,209 + GFF3095 0.38 +0.1 173,209 + GFF3095 0.38 +1.2 173,210 - GFF3095 0.38 -0.9 173,210 - GFF3095 0.38 -0.3 173,210 - GFF3095 0.38 -0.1 173,362 + GFF3095 0.76 +0.6 173,363 - GFF3095 0.76 +1.0 173,363 - GFF3095 0.76 -0.1 173,374 + GFF3095 0.79 -0.5 173,374 + GFF3095 0.79 +0.4 173,374 + GFF3095 0.79 +0.0 173,375 - GFF3095 0.79 +0.5 173,375 - GFF3095 0.79 -1.5 173,375 - GFF3095 0.79 +0.3 173,375 - GFF3095 0.79 -1.0 173,375 - GFF3095 0.79 +0.8 173,440 + -0.6 173,440 + -1.5 173,440 + +0.0 173,440 + -0.0 173,440 + +0.1 173,441 - +0.3 173,441 - -0.3 173,441 - -0.6 173,462 + -0.0 173,462 + -0.2 173,463 - -0.6
Or see this region's nucleotide sequence