Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1824 and GFF1825 are separated by 4 nucleotides GFF1825 and GFF1826 are separated by 11 nucleotides GFF1826 and GFF1827 are separated by 357 nucleotides
GFF1824 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 689,482 to 690,309
GFF1824
GFF1825 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 690,314 to 690,760
GFF1825
GFF1826 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 690,772 to 691,650
GFF1826
GFF1827 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 692,008 to 693,480
GFF1827
Position (kb)
690
691
692 Strain fitness (log2 ratio)
-1
0
1
2 at 689.901 kb on - strand, within GFF1824 at 689.901 kb on - strand, within GFF1824 at 689.901 kb on - strand, within GFF1824 at 690.513 kb on + strand, within GFF1825 at 690.514 kb on - strand, within GFF1825 at 690.514 kb on - strand, within GFF1825 at 690.514 kb on - strand, within GFF1825 at 690.526 kb on - strand, within GFF1825 at 690.526 kb on - strand, within GFF1825 at 690.738 kb on + strand at 690.739 kb on - strand at 690.794 kb on + strand at 690.795 kb on - strand at 690.926 kb on + strand, within GFF1826 at 690.927 kb on - strand, within GFF1826 at 690.927 kb on - strand, within GFF1826 at 690.927 kb on - strand, within GFF1826 at 690.927 kb on - strand, within GFF1826 at 691.001 kb on + strand, within GFF1826 at 691.001 kb on + strand, within GFF1826 at 691.002 kb on - strand, within GFF1826 at 691.181 kb on + strand, within GFF1826 at 691.181 kb on + strand, within GFF1826 at 691.182 kb on - strand, within GFF1826 at 691.182 kb on - strand, within GFF1826 at 691.298 kb on + strand, within GFF1826 at 691.298 kb on + strand, within GFF1826 at 691.299 kb on - strand, within GFF1826 at 691.299 kb on - strand, within GFF1826 at 691.322 kb on + strand, within GFF1826 at 691.469 kb on + strand, within GFF1826 at 691.469 kb on + strand, within GFF1826 at 691.469 kb on + strand, within GFF1826 at 691.469 kb on + strand, within GFF1826 at 691.469 kb on + strand, within GFF1826 at 691.470 kb on - strand, within GFF1826 at 691.470 kb on - strand, within GFF1826 at 691.470 kb on - strand, within GFF1826 at 691.505 kb on + strand, within GFF1826 at 691.505 kb on + strand, within GFF1826 at 691.506 kb on - strand, within GFF1826 at 691.506 kb on - strand, within GFF1826 at 691.506 kb on - strand, within GFF1826 at 691.506 kb on - strand, within GFF1826 at 691.506 kb on - strand, within GFF1826 at 691.646 kb on + strand at 691.647 kb on - strand at 691.647 kb on - strand at 691.647 kb on - strand at 691.684 kb on + strand at 691.717 kb on + strand at 691.780 kb on - strand at 691.780 kb on - strand at 692.081 kb on + strand at 692.082 kb on - strand at 692.082 kb on - strand at 692.237 kb on + strand, within GFF1827 at 692.237 kb on + strand, within GFF1827 at 692.237 kb on + strand, within GFF1827 at 692.237 kb on + strand, within GFF1827 at 692.238 kb on - strand, within GFF1827 at 692.238 kb on - strand, within GFF1827 at 692.238 kb on - strand, within GFF1827 at 692.238 kb on - strand, within GFF1827 at 692.238 kb on - strand, within GFF1827 at 692.252 kb on + strand, within GFF1827 at 692.253 kb on - strand, within GFF1827 at 692.387 kb on + strand, within GFF1827 at 692.387 kb on + strand, within GFF1827 at 692.388 kb on - strand, within GFF1827 at 692.388 kb on - strand, within GFF1827 at 692.427 kb on - strand, within GFF1827 at 692.427 kb on - strand, within GFF1827 at 692.489 kb on + strand, within GFF1827 at 692.489 kb on + strand, within GFF1827 at 692.490 kb on - strand, within GFF1827 at 692.490 kb on - strand, within GFF1827 at 692.490 kb on - strand, within GFF1827 at 692.490 kb on - strand, within GFF1827 at 692.613 kb on - strand, within GFF1827 at 692.613 kb on - strand, within GFF1827
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 689,901 - GFF1824 0.51 -0.0 689,901 - GFF1824 0.51 -0.0 689,901 - GFF1824 0.51 -0.2 690,513 + GFF1825 0.45 -0.5 690,514 - GFF1825 0.45 +0.4 690,514 - GFF1825 0.45 +0.5 690,514 - GFF1825 0.45 +0.5 690,526 - GFF1825 0.47 -0.3 690,526 - GFF1825 0.47 +0.1 690,738 + -0.5 690,739 - -0.9 690,794 + -0.9 690,795 - +1.3 690,926 + GFF1826 0.18 -0.5 690,927 - GFF1826 0.18 +0.1 690,927 - GFF1826 0.18 +0.0 690,927 - GFF1826 0.18 +0.9 690,927 - GFF1826 0.18 +0.2 691,001 + GFF1826 0.26 -1.3 691,001 + GFF1826 0.26 +0.3 691,002 - GFF1826 0.26 +0.3 691,181 + GFF1826 0.47 -0.4 691,181 + GFF1826 0.47 -0.3 691,182 - GFF1826 0.47 -0.6 691,182 - GFF1826 0.47 +0.2 691,298 + GFF1826 0.60 +0.2 691,298 + GFF1826 0.60 +0.1 691,299 - GFF1826 0.60 +0.4 691,299 - GFF1826 0.60 +0.1 691,322 + GFF1826 0.63 -1.1 691,469 + GFF1826 0.79 +1.0 691,469 + GFF1826 0.79 -1.3 691,469 + GFF1826 0.79 +0.1 691,469 + GFF1826 0.79 -0.0 691,469 + GFF1826 0.79 -0.1 691,470 - GFF1826 0.79 +1.1 691,470 - GFF1826 0.79 -0.1 691,470 - GFF1826 0.79 +0.0 691,505 + GFF1826 0.83 +2.2 691,505 + GFF1826 0.83 +0.5 691,506 - GFF1826 0.84 -0.2 691,506 - GFF1826 0.84 +0.0 691,506 - GFF1826 0.84 -0.2 691,506 - GFF1826 0.84 +0.4 691,506 - GFF1826 0.84 -0.1 691,646 + +0.1 691,647 - +0.5 691,647 - -1.1 691,647 - -0.0 691,684 + -0.1 691,717 + +0.3 691,780 - -0.1 691,780 - +0.2 692,081 + +0.7 692,082 - +0.1 692,082 - +0.6 692,237 + GFF1827 0.16 -0.3 692,237 + GFF1827 0.16 +0.1 692,237 + GFF1827 0.16 -0.4 692,237 + GFF1827 0.16 +0.2 692,238 - GFF1827 0.16 -0.2 692,238 - GFF1827 0.16 +0.2 692,238 - GFF1827 0.16 -0.1 692,238 - GFF1827 0.16 +0.1 692,238 - GFF1827 0.16 -0.1 692,252 + GFF1827 0.17 -0.6 692,253 - GFF1827 0.17 +0.0 692,387 + GFF1827 0.26 -0.7 692,387 + GFF1827 0.26 +0.4 692,388 - GFF1827 0.26 -0.0 692,388 - GFF1827 0.26 -0.4 692,427 - GFF1827 0.28 +0.4 692,427 - GFF1827 0.28 -1.1 692,489 + GFF1827 0.33 +0.7 692,489 + GFF1827 0.33 +1.0 692,490 - GFF1827 0.33 +0.3 692,490 - GFF1827 0.33 +0.7 692,490 - GFF1827 0.33 +1.1 692,490 - GFF1827 0.33 +0.7 692,613 - GFF1827 0.41 -0.3 692,613 - GFF1827 0.41 +0.7
Or see this region's nucleotide sequence