Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF14 and GFF15 overlap by 4 nucleotides GFF15 and GFF16 are separated by 42 nucleotides GFF16 and GFF17 are separated by 1 nucleotides GFF17 and GFF18 are separated by 105 nucleotides
GFF14 - Aldehyde dehydrogenase (EC 1.2.1.3), at 10,658 to 11,764
GFF14
GFF15 - Aldehyde dehydrogenase (EC 1.2.1.3), at 11,761 to 11,874
GFF15
GFF16 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 11,917 to 12,696
GFF16
GFF17 - LysR family transcriptional regulator YfeR, at 12,698 to 13,600
GFF17
GFF18 - Sodium/bile acid symporter family, at 13,706 to 14,725
GFF18
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1 at 12.396 kb on + strand, within GFF16 at 12.616 kb on + strand, within GFF16 at 12.641 kb on - strand at 13.220 kb on + strand, within GFF17 at 13.606 kb on - strand at 13.627 kb on - strand at 13.745 kb on + strand at 13.745 kb on + strand at 13.745 kb on + strand at 13.746 kb on - strand at 14.018 kb on + strand, within GFF18 at 14.018 kb on + strand, within GFF18 at 14.337 kb on - strand, within GFF18 at 14.337 kb on - strand, within GFF18
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 12,396 + GFF16 0.61 -0.3 12,616 + GFF16 0.90 -0.6 12,641 - +0.0 13,220 + GFF17 0.58 -0.1 13,606 - +0.1 13,627 - -0.4 13,745 + +0.6 13,745 + -1.4 13,745 + -0.5 13,746 - -0.1 14,018 + GFF18 0.31 -0.1 14,018 + GFF18 0.31 +0.0 14,337 - GFF18 0.62 +0.3 14,337 - GFF18 0.62 -0.1
Or see this region's nucleotide sequence