Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF446

Experiment: Ying_sugar7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF445 and GFF446 overlap by 17 nucleotidesGFF446 and GFF447 overlap by 45 nucleotidesGFF447 and GFF448 are separated by 32 nucleotides GFF445 - Acetolactate synthase large subunit (EC 2.2.1.6), at 114,371 to 116,251 GFF445 GFF446 - hypothetical protein, at 116,235 to 116,384 GFF446 GFF447 - Transcriptional regulator, AraC family, at 116,340 to 117,143 GFF447 GFF448 - hypothetical protein, at 117,176 to 118,393 GFF448 Position (kb) 116 117Strain fitness (log2 ratio) -2 -1 0 1 2at 115.539 kb on + strand, within GFF445at 116.325 kb on + strand, within GFF446at 116.326 kb on - strand, within GFF446at 116.326 kb on - strand, within GFF446at 116.453 kb on - strand, within GFF447at 116.511 kb on + strand, within GFF447at 116.511 kb on + strand, within GFF447at 116.512 kb on - strand, within GFF447at 116.512 kb on - strand, within GFF447at 116.874 kb on + strand, within GFF447at 116.997 kb on + strand, within GFF447at 117.054 kb on + strand, within GFF447at 117.164 kb on + strandat 117.165 kb on - strandat 117.165 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2
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115,539 + GFF445 0.62 +2.1
116,325 + GFF446 0.60 -0.4
116,326 - GFF446 0.61 +0.7
116,326 - GFF446 0.61 +0.3
116,453 - GFF447 0.14 -0.2
116,511 + GFF447 0.21 -0.4
116,511 + GFF447 0.21 +0.7
116,512 - GFF447 0.21 +0.0
116,512 - GFF447 0.21 +0.4
116,874 + GFF447 0.66 +0.0
116,997 + GFF447 0.82 +1.6
117,054 + GFF447 0.89 -0.2
117,164 + -2.0
117,165 - -0.1
117,165 - -0.2

Or see this region's nucleotide sequence