Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF112

Experiment: Ying_sugar7 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF111 and GFF112 are separated by 8 nucleotidesGFF112 and GFF113 are separated by 1 nucleotidesGFF113 and GFF114 overlap by 1 nucleotides GFF111 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 119,124 to 121,628 GFF111 GFF112 - Ku domain protein, at 121,637 to 122,470 GFF112 GFF113 - Membrane protein, putative, at 122,472 to 123,383 GFF113 GFF114 - COG0613, Predicted metal-dependent phosphoesterases (PHP family), at 123,383 to 124,240 GFF114 Position (kb) 121 122 123Strain fitness (log2 ratio) -2 -1 0 1 2at 120.945 kb on + strand, within GFF111at 121.841 kb on + strand, within GFF112at 121.841 kb on + strand, within GFF112at 121.842 kb on - strand, within GFF112at 121.842 kb on - strand, within GFF112at 121.842 kb on - strand, within GFF112at 121.842 kb on - strand, within GFF112at 121.842 kb on - strand, within GFF112at 121.842 kb on - strand, within GFF112at 121.958 kb on + strand, within GFF112at 121.958 kb on + strand, within GFF112at 121.959 kb on - strand, within GFF112at 121.959 kb on - strand, within GFF112at 121.961 kb on + strand, within GFF112at 121.961 kb on + strand, within GFF112at 121.994 kb on + strand, within GFF112at 121.994 kb on + strand, within GFF112at 121.994 kb on + strand, within GFF112at 121.994 kb on + strand, within GFF112at 121.995 kb on - strand, within GFF112at 121.995 kb on - strand, within GFF112at 121.995 kb on - strand, within GFF112at 121.995 kb on - strand, within GFF112at 122.261 kb on + strand, within GFF112at 122.261 kb on + strand, within GFF112at 122.644 kb on + strand, within GFF113at 122.644 kb on + strand, within GFF113at 122.645 kb on - strand, within GFF113at 122.818 kb on + strand, within GFF113at 122.884 kb on + strand, within GFF113at 122.884 kb on + strand, within GFF113at 122.885 kb on - strand, within GFF113at 122.885 kb on - strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.091 kb on + strand, within GFF113at 123.092 kb on - strand, within GFF113at 123.092 kb on - strand, within GFF113at 123.092 kb on - strand, within GFF113at 123.092 kb on - strand, within GFF113at 123.092 kb on - strand, within GFF113at 123.280 kb on + strand, within GFF113at 123.280 kb on + strand, within GFF113at 123.280 kb on + strand, within GFF113at 123.281 kb on - strand, within GFF113at 123.281 kb on - strand, within GFF113at 123.281 kb on - strand, within GFF113at 123.281 kb on - strand, within GFF113

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 2
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120,945 + GFF111 0.73 -1.7
121,841 + GFF112 0.24 +0.1
121,841 + GFF112 0.24 +1.0
121,842 - GFF112 0.25 -0.4
121,842 - GFF112 0.25 +0.7
121,842 - GFF112 0.25 +0.4
121,842 - GFF112 0.25 -0.5
121,842 - GFF112 0.25 +1.1
121,842 - GFF112 0.25 -0.1
121,958 + GFF112 0.38 -1.3
121,958 + GFF112 0.38 +0.0
121,959 - GFF112 0.39 +1.0
121,959 - GFF112 0.39 -0.7
121,961 + GFF112 0.39 -0.5
121,961 + GFF112 0.39 +0.4
121,994 + GFF112 0.43 +0.3
121,994 + GFF112 0.43 -0.2
121,994 + GFF112 0.43 -0.8
121,994 + GFF112 0.43 -0.5
121,995 - GFF112 0.43 -0.1
121,995 - GFF112 0.43 +0.4
121,995 - GFF112 0.43 -0.5
121,995 - GFF112 0.43 -0.5
122,261 + GFF112 0.75 -0.4
122,261 + GFF112 0.75 -0.6
122,644 + GFF113 0.19 +0.3
122,644 + GFF113 0.19 -0.1
122,645 - GFF113 0.19 -0.2
122,818 + GFF113 0.38 -0.5
122,884 + GFF113 0.45 +2.0
122,884 + GFF113 0.45 -0.8
122,885 - GFF113 0.45 -0.2
122,885 - GFF113 0.45 -0.0
123,091 + GFF113 0.68 +0.3
123,091 + GFF113 0.68 +0.3
123,091 + GFF113 0.68 +0.8
123,091 + GFF113 0.68 +0.3
123,091 + GFF113 0.68 +0.2
123,091 + GFF113 0.68 +0.4
123,091 + GFF113 0.68 +0.7
123,091 + GFF113 0.68 +0.4
123,091 + GFF113 0.68 -0.2
123,092 - GFF113 0.68 -0.6
123,092 - GFF113 0.68 +0.7
123,092 - GFF113 0.68 +0.4
123,092 - GFF113 0.68 -0.2
123,092 - GFF113 0.68 -1.8
123,280 + GFF113 0.89 -0.2
123,280 + GFF113 0.89 +2.1
123,280 + GFF113 0.89 -0.3
123,281 - GFF113 0.89 +0.1
123,281 - GFF113 0.89 +1.0
123,281 - GFF113 0.89 -0.0
123,281 - GFF113 0.89 +0.9

Or see this region's nucleotide sequence