Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS21475 and vioA are separated by 202 nucleotides vioA and COO64_RS21485 overlap by 20 nucleotides COO64_RS21485 and COO64_RS21490 are separated by 118 nucleotides
COO64_RS21475: COO64_RS21475 - hypothetical protein, at 60,681 to 61,259
_RS21475
COO64_RS21480: vioA - dTDP-4-amino-4,6-dideoxy-D-glucose aminotransferase VioA, at 61,462 to 62,613
vioA
COO64_RS21485: COO64_RS21485 - LysR substrate-binding domain-containing protein, at 62,594 to 63,475
_RS21485
COO64_RS21490: COO64_RS21490 - glutathione S-transferase family protein, at 63,594 to 64,220
_RS21490
Position (kb) 61
62
63 Strain fitness (log2 ratio) -2
-1
0
1 at 60.573 kb on + strand at 60.574 kb on - strand at 60.574 kb on - strand at 60.597 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.687 kb on - strand at 60.687 kb on - strand at 60.687 kb on - strand at 60.975 kb on + strand, within COO64_RS21475 at 60.975 kb on + strand, within COO64_RS21475 at 60.975 kb on + strand, within COO64_RS21475 at 60.975 kb on + strand, within COO64_RS21475 at 60.975 kb on + strand, within COO64_RS21475 at 60.976 kb on - strand, within COO64_RS21475 at 60.976 kb on - strand, within COO64_RS21475 at 61.339 kb on - strand at 61.522 kb on + strand at 61.522 kb on + strand at 61.523 kb on - strand at 61.603 kb on + strand, within vioA at 61.604 kb on - strand, within vioA at 61.705 kb on + strand, within vioA at 61.705 kb on + strand, within vioA at 61.706 kb on - strand, within vioA at 61.706 kb on - strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.904 kb on - strand, within vioA at 61.904 kb on - strand, within vioA at 61.904 kb on - strand, within vioA at 61.904 kb on - strand, within vioA at 61.904 kb on - strand, within vioA at 61.921 kb on + strand, within vioA at 61.921 kb on + strand, within vioA at 61.921 kb on + strand, within vioA at 61.921 kb on + strand, within vioA at 61.922 kb on - strand, within vioA at 61.922 kb on - strand, within vioA at 61.922 kb on - strand, within vioA at 61.922 kb on - strand, within vioA at 61.922 kb on - strand, within vioA at 62.080 kb on + strand, within vioA at 62.231 kb on - strand, within vioA at 62.253 kb on - strand, within vioA at 62.299 kb on + strand, within vioA at 62.299 kb on + strand, within vioA at 62.300 kb on - strand, within vioA at 62.300 kb on - strand, within vioA at 62.300 kb on - strand, within vioA at 62.300 kb on - strand, within vioA at 62.306 kb on - strand, within vioA at 62.306 kb on - strand, within vioA at 62.306 kb on - strand, within vioA at 62.360 kb on - strand, within vioA at 62.360 kb on - strand, within vioA at 62.398 kb on + strand, within vioA at 62.398 kb on + strand, within vioA at 62.399 kb on - strand, within vioA at 62.399 kb on - strand, within vioA at 62.404 kb on + strand, within vioA at 62.432 kb on - strand, within vioA at 62.495 kb on + strand, within vioA at 62.495 kb on + strand, within vioA at 62.496 kb on - strand, within vioA at 62.534 kb on + strand at 62.535 kb on - strand at 62.535 kb on - strand at 62.595 kb on + strand at 62.596 kb on - strand at 62.673 kb on + strand at 62.673 kb on + strand at 62.674 kb on - strand at 62.676 kb on + strand at 62.676 kb on + strand at 62.677 kb on - strand at 62.914 kb on - strand, within COO64_RS21485 at 62.988 kb on - strand, within COO64_RS21485 at 63.270 kb on + strand, within COO64_RS21485 at 63.479 kb on + strand at 63.479 kb on + strand at 63.480 kb on - strand at 63.480 kb on - strand at 63.480 kb on - strand at 63.553 kb on - strand at 63.553 kb on - strand at 63.557 kb on + strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.576 kb on + strand at 63.576 kb on + strand at 63.576 kb on + strand at 63.576 kb on + strand at 63.577 kb on - strand at 63.577 kb on - strand at 63.577 kb on - strand at 63.610 kb on - strand at 63.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.1 X remove 60,573 + -0.6 60,574 - -0.2 60,574 - +0.3 60,597 + +0.1 60,686 + +0.1 60,686 + -0.9 60,686 + -0.8 60,686 + +0.0 60,686 + -0.0 60,686 + +0.7 60,687 - -1.8 60,687 - -0.5 60,687 - +1.2 60,975 + COO64_RS21475 0.51 +0.5 60,975 + COO64_RS21475 0.51 +0.0 60,975 + COO64_RS21475 0.51 +1.0 60,975 + COO64_RS21475 0.51 +0.4 60,975 + COO64_RS21475 0.51 -0.5 60,976 - COO64_RS21475 0.51 -0.2 60,976 - COO64_RS21475 0.51 -0.1 61,339 - +0.0 61,522 + -1.0 61,522 + -0.6 61,523 - +0.1 61,603 + vioA COO64_RS21480 0.12 +1.5 61,604 - vioA COO64_RS21480 0.12 +0.9 61,705 + vioA COO64_RS21480 0.21 -0.7 61,705 + vioA COO64_RS21480 0.21 +0.4 61,706 - vioA COO64_RS21480 0.21 -0.1 61,706 - vioA COO64_RS21480 0.21 +0.4 61,903 + vioA COO64_RS21480 0.38 -0.0 61,903 + vioA COO64_RS21480 0.38 -0.8 61,903 + vioA COO64_RS21480 0.38 +0.4 61,903 + vioA COO64_RS21480 0.38 -1.7 61,903 + vioA COO64_RS21480 0.38 +1.3 61,903 + vioA COO64_RS21480 0.38 +0.1 61,903 + vioA COO64_RS21480 0.38 -0.4 61,903 + vioA COO64_RS21480 0.38 +0.3 61,904 - vioA COO64_RS21480 0.38 -2.4 61,904 - vioA COO64_RS21480 0.38 -1.0 61,904 - vioA COO64_RS21480 0.38 +0.1 61,904 - vioA COO64_RS21480 0.38 -0.7 61,904 - vioA COO64_RS21480 0.38 +0.4 61,921 + vioA COO64_RS21480 0.40 +0.6 61,921 + vioA COO64_RS21480 0.40 -0.3 61,921 + vioA COO64_RS21480 0.40 +0.7 61,921 + vioA COO64_RS21480 0.40 -0.2 61,922 - vioA COO64_RS21480 0.40 -0.2 61,922 - vioA COO64_RS21480 0.40 -1.4 61,922 - vioA COO64_RS21480 0.40 -1.4 61,922 - vioA COO64_RS21480 0.40 +0.3 61,922 - vioA COO64_RS21480 0.40 -0.3 62,080 + vioA COO64_RS21480 0.54 -0.6 62,231 - vioA COO64_RS21480 0.67 -0.1 62,253 - vioA COO64_RS21480 0.69 -0.0 62,299 + vioA COO64_RS21480 0.73 +0.9 62,299 + vioA COO64_RS21480 0.73 -0.4 62,300 - vioA COO64_RS21480 0.73 +1.5 62,300 - vioA COO64_RS21480 0.73 +0.1 62,300 - vioA COO64_RS21480 0.73 -0.5 62,300 - vioA COO64_RS21480 0.73 -0.3 62,306 - vioA COO64_RS21480 0.73 +0.3 62,306 - vioA COO64_RS21480 0.73 -0.9 62,306 - vioA COO64_RS21480 0.73 +1.0 62,360 - vioA COO64_RS21480 0.78 -2.0 62,360 - vioA COO64_RS21480 0.78 +0.6 62,398 + vioA COO64_RS21480 0.81 -0.8 62,398 + vioA COO64_RS21480 0.81 -0.2 62,399 - vioA COO64_RS21480 0.81 +0.3 62,399 - vioA COO64_RS21480 0.81 -2.7 62,404 + vioA COO64_RS21480 0.82 -1.3 62,432 - vioA COO64_RS21480 0.84 +0.9 62,495 + vioA COO64_RS21480 0.90 +0.2 62,495 + vioA COO64_RS21480 0.90 +0.0 62,496 - vioA COO64_RS21480 0.90 -0.6 62,534 + +1.3 62,535 - -0.5 62,535 - -0.9 62,595 + -1.0 62,596 - -0.2 62,673 + +0.1 62,673 + -1.3 62,674 - -0.6 62,676 + -0.9 62,676 + +0.4 62,677 - -1.9 62,914 - COO64_RS21485 0.36 -0.0 62,988 - COO64_RS21485 0.45 -0.6 63,270 + COO64_RS21485 0.77 +0.3 63,479 + +0.0 63,479 + -0.3 63,480 - +0.6 63,480 - +0.4 63,480 - -0.4 63,553 - -0.2 63,553 - -0.8 63,557 + -1.0 63,558 - -0.8 63,558 - +0.8 63,558 - +0.5 63,558 - +0.1 63,558 - -1.6 63,558 - -0.0 63,558 - -1.0 63,558 - +0.4 63,558 - -0.6 63,576 + -1.2 63,576 + -1.1 63,576 + +0.1 63,576 + -0.6 63,577 - +0.0 63,577 - +0.2 63,577 - -0.2 63,610 - -0.4 63,611 + +1.0
Or see this region's nucleotide sequence