Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gdhA and COO64_RS03015 overlap by 4 nucleotides COO64_RS03015 and COO64_RS03020 are separated by 66 nucleotides COO64_RS03020 and COO64_RS03025 are separated by 184 nucleotides
COO64_RS03010: gdhA - NADP-specific glutamate dehydrogenase, at 83,553 to 84,890
gdhA
COO64_RS03015: COO64_RS03015 - GreA/GreB family elongation factor, at 84,887 to 85,384
_RS03015
COO64_RS03020: COO64_RS03020 - hypothetical protein, at 85,451 to 85,807
_RS03020
COO64_RS03025: COO64_RS03025 - DUF4105 domain-containing protein, at 85,992 to 87,845
_RS03025
Position (kb) 84
85
86 Strain fitness (log2 ratio) -2
-1
0
1
2 at 84.093 kb on + strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.147 kb on + strand, within gdhA at 84.147 kb on + strand, within gdhA at 84.148 kb on - strand, within gdhA at 84.195 kb on + strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.282 kb on + strand, within gdhA at 84.282 kb on + strand, within gdhA at 84.283 kb on - strand, within gdhA at 84.283 kb on - strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.352 kb on - strand, within gdhA at 84.617 kb on + strand, within gdhA at 84.617 kb on + strand, within gdhA at 84.792 kb on + strand at 84.793 kb on - strand at 84.793 kb on - strand at 84.819 kb on + strand at 84.820 kb on - strand at 84.820 kb on - strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.889 kb on - strand at 84.889 kb on - strand at 84.889 kb on - strand at 85.026 kb on + strand, within COO64_RS03015 at 85.026 kb on + strand, within COO64_RS03015 at 85.027 kb on - strand, within COO64_RS03015 at 85.168 kb on - strand, within COO64_RS03015 at 85.168 kb on - strand, within COO64_RS03015 at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.537 kb on - strand, within COO64_RS03020 at 85.605 kb on + strand, within COO64_RS03020 at 85.606 kb on - strand, within COO64_RS03020 at 85.606 kb on - strand, within COO64_RS03020 at 85.624 kb on - strand, within COO64_RS03020 at 85.624 kb on - strand, within COO64_RS03020 at 85.778 kb on + strand at 85.779 kb on - strand at 85.838 kb on + strand at 85.839 kb on - strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.941 kb on - strand at 85.941 kb on - strand at 85.941 kb on - strand at 85.941 kb on - strand at 85.993 kb on + strand at 85.993 kb on + strand at 85.993 kb on + strand at 85.993 kb on + strand at 85.994 kb on - strand at 85.994 kb on - strand at 85.994 kb on - strand at 85.994 kb on - strand at 85.994 kb on - strand at 85.996 kb on + strand at 85.996 kb on + strand at 85.997 kb on - strand at 85.997 kb on - strand at 85.997 kb on - strand at 85.997 kb on - strand at 86.002 kb on + strand at 86.149 kb on + strand at 86.150 kb on - strand at 86.150 kb on - strand at 86.312 kb on + strand, within COO64_RS03025 at 86.346 kb on + strand, within COO64_RS03025 at 86.347 kb on - strand, within COO64_RS03025 at 86.347 kb on - strand, within COO64_RS03025 at 86.347 kb on - strand, within COO64_RS03025
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.1 X remove 84,093 + gdhA COO64_RS03010 0.40 +1.3 84,093 + gdhA COO64_RS03010 0.40 +0.5 84,093 + gdhA COO64_RS03010 0.40 +0.2 84,094 - gdhA COO64_RS03010 0.40 -0.4 84,094 - gdhA COO64_RS03010 0.40 -0.3 84,094 - gdhA COO64_RS03010 0.40 -0.1 84,147 + gdhA COO64_RS03010 0.44 +1.4 84,147 + gdhA COO64_RS03010 0.44 +0.3 84,148 - gdhA COO64_RS03010 0.44 -0.0 84,195 + gdhA COO64_RS03010 0.48 +0.5 84,196 - gdhA COO64_RS03010 0.48 -0.2 84,196 - gdhA COO64_RS03010 0.48 -0.9 84,196 - gdhA COO64_RS03010 0.48 -0.1 84,282 + gdhA COO64_RS03010 0.54 -0.6 84,282 + gdhA COO64_RS03010 0.54 +0.5 84,283 - gdhA COO64_RS03010 0.55 -0.1 84,283 - gdhA COO64_RS03010 0.55 +0.2 84,351 + gdhA COO64_RS03010 0.60 +0.1 84,351 + gdhA COO64_RS03010 0.60 +0.8 84,351 + gdhA COO64_RS03010 0.60 -0.3 84,351 + gdhA COO64_RS03010 0.60 +2.0 84,352 - gdhA COO64_RS03010 0.60 -1.3 84,617 + gdhA COO64_RS03010 0.80 +0.2 84,617 + gdhA COO64_RS03010 0.80 -0.5 84,792 + +1.2 84,793 - -1.9 84,793 - +0.7 84,819 + -1.8 84,820 - +0.5 84,820 - -1.1 84,888 + -0.0 84,888 + -0.7 84,888 + +0.1 84,888 + +1.2 84,889 - -1.2 84,889 - +0.6 84,889 - +0.1 85,026 + COO64_RS03015 0.28 -0.5 85,026 + COO64_RS03015 0.28 +1.2 85,027 - COO64_RS03015 0.28 +0.8 85,168 - COO64_RS03015 0.56 -0.5 85,168 - COO64_RS03015 0.56 +0.9 85,452 + +1.4 85,452 + +1.8 85,452 + +0.5 85,452 + +0.2 85,452 + +0.2 85,452 + -0.0 85,452 + +0.1 85,453 - -0.1 85,453 - +0.9 85,453 - -0.2 85,453 - +0.8 85,537 - COO64_RS03020 0.24 -0.2 85,605 + COO64_RS03020 0.43 +0.5 85,606 - COO64_RS03020 0.43 -0.9 85,606 - COO64_RS03020 0.43 -1.4 85,624 - COO64_RS03020 0.48 +0.2 85,624 - COO64_RS03020 0.48 +0.8 85,778 + -1.8 85,779 - -0.4 85,838 + -0.1 85,839 - +1.8 85,940 + -0.2 85,940 + +0.2 85,940 + -0.4 85,940 + +0.3 85,940 + -0.6 85,940 + -0.7 85,940 + +0.4 85,940 + +0.3 85,941 - +0.1 85,941 - +0.2 85,941 - +1.6 85,941 - -0.5 85,993 + +0.7 85,993 + +0.5 85,993 + -0.7 85,993 + -0.2 85,994 - +0.5 85,994 - +1.8 85,994 - -1.3 85,994 - +0.2 85,994 - -0.0 85,996 + +0.8 85,996 + -0.4 85,997 - -1.0 85,997 - -0.7 85,997 - +0.8 85,997 - +0.8 86,002 + +0.8 86,149 + -0.3 86,150 - -0.2 86,150 - -0.4 86,312 + COO64_RS03025 0.17 +0.8 86,346 + COO64_RS03025 0.19 +0.3 86,347 - COO64_RS03025 0.19 +0.1 86,347 - COO64_RS03025 0.19 +0.2 86,347 - COO64_RS03025 0.19 -1.0
Or see this region's nucleotide sequence