Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt araC and yciW are separated by 139 nucleotides yciW and OKGIIK_14580 are separated by 114 nucleotides OKGIIK_14580 and OKGIIK_14585 are separated by 122 nucleotides OKGIIK_14585 and yigB overlap by 14 nucleotides
OKGIIK_14570: araC - AraC family transcriptional regulator, at 3,182,738 to 3,183,592
araC
OKGIIK_14575: yciW - carboxymuconolactone decarboxylase, at 3,183,732 to 3,184,289
yciW
OKGIIK_14580: OKGIIK_14580 - EthD family reductase, at 3,184,404 to 3,184,724
_14580
OKGIIK_14585: OKGIIK_14585 - hypothetical protein, at 3,184,847 to 3,185,266
_14585
OKGIIK_14590: yigB - haloacid dehalogenase type II, at 3,185,253 to 3,186,011
yigB
Position (kb)
3184
3185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3183.495 kb on + strand, within araC at 3183.496 kb on - strand, within araC at 3183.496 kb on - strand, within araC at 3183.670 kb on + strand at 3183.671 kb on - strand at 3183.671 kb on - strand at 3183.724 kb on + strand at 3183.724 kb on + strand at 3183.724 kb on + strand at 3183.724 kb on + strand at 3183.724 kb on + strand at 3183.725 kb on - strand at 3183.725 kb on - strand at 3183.725 kb on - strand at 3183.725 kb on - strand at 3183.725 kb on - strand at 3183.831 kb on + strand, within yciW at 3183.831 kb on + strand, within yciW at 3183.832 kb on - strand, within yciW at 3183.832 kb on - strand, within yciW at 3183.832 kb on - strand, within yciW at 3184.219 kb on - strand, within yciW at 3184.310 kb on + strand at 3184.311 kb on - strand at 3184.311 kb on - strand at 3184.393 kb on - strand at 3184.393 kb on - strand at 3184.393 kb on - strand at 3184.393 kb on - strand at 3184.425 kb on + strand at 3184.425 kb on + strand at 3184.425 kb on + strand at 3184.426 kb on - strand at 3184.426 kb on - strand at 3184.426 kb on - strand at 3184.426 kb on - strand at 3184.431 kb on + strand at 3184.432 kb on - strand at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.461 kb on + strand, within OKGIIK_14580 at 3184.462 kb on - strand, within OKGIIK_14580 at 3184.462 kb on - strand, within OKGIIK_14580 at 3184.462 kb on - strand, within OKGIIK_14580 at 3184.462 kb on - strand, within OKGIIK_14580 at 3184.464 kb on + strand, within OKGIIK_14580 at 3184.465 kb on - strand, within OKGIIK_14580 at 3184.465 kb on - strand, within OKGIIK_14580 at 3184.518 kb on + strand, within OKGIIK_14580 at 3184.518 kb on + strand, within OKGIIK_14580 at 3184.518 kb on + strand, within OKGIIK_14580 at 3184.518 kb on + strand, within OKGIIK_14580 at 3184.518 kb on + strand, within OKGIIK_14580 at 3184.518 kb on + strand, within OKGIIK_14580 at 3184.519 kb on - strand, within OKGIIK_14580 at 3184.519 kb on - strand, within OKGIIK_14580 at 3184.519 kb on - strand, within OKGIIK_14580 at 3184.521 kb on + strand, within OKGIIK_14580 at 3184.521 kb on + strand, within OKGIIK_14580 at 3184.522 kb on - strand, within OKGIIK_14580 at 3184.522 kb on - strand, within OKGIIK_14580 at 3184.522 kb on - strand, within OKGIIK_14580 at 3184.522 kb on - strand, within OKGIIK_14580 at 3184.522 kb on - strand, within OKGIIK_14580 at 3184.572 kb on + strand, within OKGIIK_14580 at 3184.722 kb on + strand at 3184.815 kb on - strand at 3184.820 kb on + strand at 3184.820 kb on + strand at 3184.820 kb on + strand at 3184.820 kb on + strand at 3184.820 kb on + strand at 3184.820 kb on + strand at 3184.821 kb on - strand at 3184.821 kb on - strand at 3184.821 kb on - strand at 3184.821 kb on - strand at 3184.821 kb on - strand at 3184.821 kb on - strand at 3184.821 kb on - strand at 3184.826 kb on + strand at 3184.826 kb on + strand at 3184.826 kb on + strand at 3184.826 kb on + strand at 3184.826 kb on + strand at 3184.826 kb on + strand at 3184.826 kb on + strand at 3184.827 kb on - strand at 3184.827 kb on - strand at 3184.827 kb on - strand at 3184.827 kb on - strand at 3184.827 kb on - strand at 3184.827 kb on - strand at 3184.827 kb on - strand at 3184.962 kb on + strand, within OKGIIK_14585 at 3184.962 kb on + strand, within OKGIIK_14585 at 3184.963 kb on - strand, within OKGIIK_14585 at 3184.963 kb on - strand, within OKGIIK_14585 at 3184.963 kb on - strand, within OKGIIK_14585 at 3184.976 kb on + strand, within OKGIIK_14585 at 3184.977 kb on - strand, within OKGIIK_14585 at 3184.977 kb on - strand, within OKGIIK_14585 at 3184.992 kb on + strand, within OKGIIK_14585 at 3184.992 kb on + strand, within OKGIIK_14585 at 3184.992 kb on + strand, within OKGIIK_14585 at 3184.993 kb on - strand, within OKGIIK_14585 at 3184.993 kb on - strand, within OKGIIK_14585 at 3184.993 kb on - strand, within OKGIIK_14585 at 3185.078 kb on + strand, within OKGIIK_14585 at 3185.078 kb on + strand, within OKGIIK_14585 at 3185.078 kb on + strand, within OKGIIK_14585 at 3185.078 kb on + strand, within OKGIIK_14585 at 3185.079 kb on - strand, within OKGIIK_14585 at 3185.079 kb on - strand, within OKGIIK_14585 at 3185.132 kb on + strand, within OKGIIK_14585 at 3185.132 kb on + strand, within OKGIIK_14585 at 3185.132 kb on + strand, within OKGIIK_14585 at 3185.133 kb on - strand, within OKGIIK_14585 at 3185.207 kb on + strand, within OKGIIK_14585 at 3185.207 kb on + strand, within OKGIIK_14585 at 3185.207 kb on + strand, within OKGIIK_14585 at 3185.208 kb on - strand, within OKGIIK_14585 at 3185.208 kb on - strand, within OKGIIK_14585 at 3185.208 kb on - strand, within OKGIIK_14585 at 3185.208 kb on - strand, within OKGIIK_14585 at 3185.273 kb on + strand at 3185.273 kb on + strand at 3185.274 kb on - strand at 3185.284 kb on + strand at 3185.284 kb on + strand at 3185.284 kb on + strand at 3185.284 kb on + strand at 3185.284 kb on + strand at 3185.284 kb on + strand at 3185.284 kb on + strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.285 kb on - strand at 3185.332 kb on - strand, within yigB at 3185.404 kb on + strand, within yigB at 3185.405 kb on - strand, within yigB at 3185.405 kb on - strand, within yigB at 3185.405 kb on - strand, within yigB at 3185.436 kb on + strand, within yigB at 3185.437 kb on - strand, within yigB at 3185.641 kb on + strand, within yigB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x remove 3,183,495 + araC OKGIIK_14570 0.89 -1.1 3,183,496 - araC OKGIIK_14570 0.89 -1.1 3,183,496 - araC OKGIIK_14570 0.89 +0.8 3,183,670 + -1.0 3,183,671 - -0.6 3,183,671 - +0.7 3,183,724 + -1.0 3,183,724 + +0.1 3,183,724 + -0.2 3,183,724 + +0.5 3,183,724 + +0.1 3,183,725 - +0.6 3,183,725 - -1.5 3,183,725 - -1.8 3,183,725 - +0.5 3,183,725 - -1.3 3,183,831 + yciW OKGIIK_14575 0.18 +1.0 3,183,831 + yciW OKGIIK_14575 0.18 +0.3 3,183,832 - yciW OKGIIK_14575 0.18 -0.6 3,183,832 - yciW OKGIIK_14575 0.18 -0.0 3,183,832 - yciW OKGIIK_14575 0.18 +0.3 3,184,219 - yciW OKGIIK_14575 0.87 -0.0 3,184,310 + +0.9 3,184,311 - -0.9 3,184,311 - -0.3 3,184,393 - -0.9 3,184,393 - +0.4 3,184,393 - -0.2 3,184,393 - -1.3 3,184,425 + +0.2 3,184,425 + +0.3 3,184,425 + +0.8 3,184,426 - -0.9 3,184,426 - -0.8 3,184,426 - -0.9 3,184,426 - +0.3 3,184,431 + +0.4 3,184,432 - -1.0 3,184,461 + OKGIIK_14580 0.18 -0.5 3,184,461 + OKGIIK_14580 0.18 -0.1 3,184,461 + OKGIIK_14580 0.18 -0.0 3,184,461 + OKGIIK_14580 0.18 +1.0 3,184,461 + OKGIIK_14580 0.18 +1.4 3,184,461 + OKGIIK_14580 0.18 -0.1 3,184,461 + OKGIIK_14580 0.18 +1.2 3,184,461 + OKGIIK_14580 0.18 +0.1 3,184,461 + OKGIIK_14580 0.18 -0.5 3,184,461 + OKGIIK_14580 0.18 -1.5 3,184,461 + OKGIIK_14580 0.18 -2.5 3,184,461 + OKGIIK_14580 0.18 +0.6 3,184,461 + OKGIIK_14580 0.18 +0.5 3,184,462 - OKGIIK_14580 0.18 -2.5 3,184,462 - OKGIIK_14580 0.18 -0.9 3,184,462 - OKGIIK_14580 0.18 +0.0 3,184,462 - OKGIIK_14580 0.18 -0.4 3,184,464 + OKGIIK_14580 0.19 +0.1 3,184,465 - OKGIIK_14580 0.19 +0.8 3,184,465 - OKGIIK_14580 0.19 -0.1 3,184,518 + OKGIIK_14580 0.36 +0.4 3,184,518 + OKGIIK_14580 0.36 +0.4 3,184,518 + OKGIIK_14580 0.36 +0.6 3,184,518 + OKGIIK_14580 0.36 -0.2 3,184,518 + OKGIIK_14580 0.36 -1.0 3,184,518 + OKGIIK_14580 0.36 -0.0 3,184,519 - OKGIIK_14580 0.36 -2.6 3,184,519 - OKGIIK_14580 0.36 -0.1 3,184,519 - OKGIIK_14580 0.36 -0.0 3,184,521 + OKGIIK_14580 0.36 +0.0 3,184,521 + OKGIIK_14580 0.36 -1.6 3,184,522 - OKGIIK_14580 0.37 -0.0 3,184,522 - OKGIIK_14580 0.37 -0.4 3,184,522 - OKGIIK_14580 0.37 -0.6 3,184,522 - OKGIIK_14580 0.37 +1.0 3,184,522 - OKGIIK_14580 0.37 -1.6 3,184,572 + OKGIIK_14580 0.52 -0.4 3,184,722 + +1.3 3,184,815 - -0.7 3,184,820 + +1.3 3,184,820 + -1.3 3,184,820 + -0.6 3,184,820 + -1.3 3,184,820 + +0.3 3,184,820 + +0.0 3,184,821 - -0.5 3,184,821 - +1.3 3,184,821 - +0.5 3,184,821 - -0.0 3,184,821 - -1.3 3,184,821 - +1.0 3,184,821 - -0.3 3,184,826 + -0.1 3,184,826 + -0.5 3,184,826 + -1.3 3,184,826 + -1.0 3,184,826 + +1.3 3,184,826 + +1.1 3,184,826 + -0.9 3,184,827 - -0.4 3,184,827 - -1.0 3,184,827 - -0.1 3,184,827 - -1.3 3,184,827 - -0.6 3,184,827 - +0.3 3,184,827 - -0.4 3,184,962 + OKGIIK_14585 0.27 -0.3 3,184,962 + OKGIIK_14585 0.27 -0.2 3,184,963 - OKGIIK_14585 0.28 +0.1 3,184,963 - OKGIIK_14585 0.28 +0.2 3,184,963 - OKGIIK_14585 0.28 -1.6 3,184,976 + OKGIIK_14585 0.31 -0.2 3,184,977 - OKGIIK_14585 0.31 +0.4 3,184,977 - OKGIIK_14585 0.31 -1.4 3,184,992 + OKGIIK_14585 0.35 +1.3 3,184,992 + OKGIIK_14585 0.35 -1.2 3,184,992 + OKGIIK_14585 0.35 -1.3 3,184,993 - OKGIIK_14585 0.35 -1.3 3,184,993 - OKGIIK_14585 0.35 -0.2 3,184,993 - OKGIIK_14585 0.35 -0.2 3,185,078 + OKGIIK_14585 0.55 +0.6 3,185,078 + OKGIIK_14585 0.55 -1.3 3,185,078 + OKGIIK_14585 0.55 -1.3 3,185,078 + OKGIIK_14585 0.55 +2.4 3,185,079 - OKGIIK_14585 0.55 -0.3 3,185,079 - OKGIIK_14585 0.55 -0.5 3,185,132 + OKGIIK_14585 0.68 -0.4 3,185,132 + OKGIIK_14585 0.68 -1.5 3,185,132 + OKGIIK_14585 0.68 -1.0 3,185,133 - OKGIIK_14585 0.68 -2.3 3,185,207 + OKGIIK_14585 0.86 -3.4 3,185,207 + OKGIIK_14585 0.86 -0.5 3,185,207 + OKGIIK_14585 0.86 -0.0 3,185,208 - OKGIIK_14585 0.86 -0.3 3,185,208 - OKGIIK_14585 0.86 -0.0 3,185,208 - OKGIIK_14585 0.86 +0.6 3,185,208 - OKGIIK_14585 0.86 -0.3 3,185,273 + -0.4 3,185,273 + +0.3 3,185,274 - -0.0 3,185,284 + -1.6 3,185,284 + -0.6 3,185,284 + +0.5 3,185,284 + -1.3 3,185,284 + -0.5 3,185,284 + -0.8 3,185,284 + -0.2 3,185,285 - +1.5 3,185,285 - -0.4 3,185,285 - +0.6 3,185,285 - -1.1 3,185,285 - +0.6 3,185,285 - -1.0 3,185,285 - -0.0 3,185,285 - -0.4 3,185,285 - +0.2 3,185,285 - -0.2 3,185,285 - -2.3 3,185,332 - yigB OKGIIK_14590 0.10 -1.3 3,185,404 + yigB OKGIIK_14590 0.20 +0.8 3,185,405 - yigB OKGIIK_14590 0.20 -0.0 3,185,405 - yigB OKGIIK_14590 0.20 +0.4 3,185,405 - yigB OKGIIK_14590 0.20 -0.3 3,185,436 + yigB OKGIIK_14590 0.24 +0.9 3,185,437 - yigB OKGIIK_14590 0.24 -2.9 3,185,641 + yigB OKGIIK_14590 0.51 -2.2
Or see this region's nucleotide sequence