Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truD and mgtE are separated by 159 nucleotides mgtE and ispF overlap by 4 nucleotides ispF and ispD are separated by 10 nucleotides
OKGIIK_08130: truD - tRNA pseudouridine(13) synthase TruD, at 1,787,515 to 1,788,531
truD
OKGIIK_08135: mgtE - magnesium transporter, at 1,788,691 to 1,790,019
mgtE
OKGIIK_08140: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,790,016 to 1,790,489
ispF
OKGIIK_08145: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,790,500 to 1,791,198
ispD
Position (kb)
1788
1789
1790
1791 Strain fitness (log2 ratio)
-2
-1
0
1 at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.317 kb on + strand, within truD at 1788.318 kb on - strand, within truD at 1788.437 kb on + strand at 1788.437 kb on + strand at 1788.438 kb on - strand at 1788.438 kb on - strand at 1788.438 kb on - strand at 1788.516 kb on - strand at 1788.516 kb on - strand at 1788.516 kb on - strand at 1788.516 kb on - strand at 1788.555 kb on - strand at 1788.555 kb on - strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.659 kb on + strand at 1788.659 kb on + strand at 1788.659 kb on + strand at 1788.830 kb on + strand, within mgtE at 1788.830 kb on + strand, within mgtE at 1788.830 kb on + strand, within mgtE at 1788.830 kb on + strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1789.296 kb on - strand, within mgtE at 1789.322 kb on + strand, within mgtE at 1789.322 kb on + strand, within mgtE at 1789.322 kb on + strand, within mgtE at 1789.322 kb on + strand, within mgtE at 1789.322 kb on + strand, within mgtE at 1789.323 kb on - strand, within mgtE at 1789.323 kb on - strand, within mgtE at 1789.323 kb on - strand, within mgtE at 1789.323 kb on - strand, within mgtE at 1789.499 kb on + strand, within mgtE at 1789.499 kb on + strand, within mgtE at 1789.499 kb on + strand, within mgtE at 1789.500 kb on - strand, within mgtE at 1789.583 kb on + strand, within mgtE at 1789.583 kb on + strand, within mgtE at 1789.584 kb on - strand, within mgtE at 1789.584 kb on - strand, within mgtE at 1789.584 kb on - strand, within mgtE
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x remove 1,787,938 + truD OKGIIK_08130 0.42 -0.3 1,787,938 + truD OKGIIK_08130 0.42 +0.6 1,787,938 + truD OKGIIK_08130 0.42 -0.4 1,787,938 + truD OKGIIK_08130 0.42 +0.8 1,787,938 + truD OKGIIK_08130 0.42 -0.3 1,787,938 + truD OKGIIK_08130 0.42 +1.2 1,787,938 + truD OKGIIK_08130 0.42 +0.6 1,787,938 + truD OKGIIK_08130 0.42 -1.9 1,787,939 - truD OKGIIK_08130 0.42 -1.3 1,787,939 - truD OKGIIK_08130 0.42 -0.7 1,787,939 - truD OKGIIK_08130 0.42 +0.1 1,787,939 - truD OKGIIK_08130 0.42 -0.2 1,787,939 - truD OKGIIK_08130 0.42 -1.3 1,787,939 - truD OKGIIK_08130 0.42 -0.8 1,787,939 - truD OKGIIK_08130 0.42 -2.5 1,787,939 - truD OKGIIK_08130 0.42 +0.1 1,787,939 - truD OKGIIK_08130 0.42 +0.6 1,787,939 - truD OKGIIK_08130 0.42 -0.3 1,788,062 + truD OKGIIK_08130 0.54 +0.6 1,788,062 + truD OKGIIK_08130 0.54 +0.6 1,788,062 + truD OKGIIK_08130 0.54 -1.1 1,788,062 + truD OKGIIK_08130 0.54 -1.9 1,788,062 + truD OKGIIK_08130 0.54 -0.7 1,788,062 + truD OKGIIK_08130 0.54 -1.5 1,788,062 + truD OKGIIK_08130 0.54 +0.4 1,788,062 + truD OKGIIK_08130 0.54 -1.4 1,788,062 + truD OKGIIK_08130 0.54 -0.7 1,788,062 + truD OKGIIK_08130 0.54 -0.4 1,788,062 + truD OKGIIK_08130 0.54 -0.4 1,788,062 + truD OKGIIK_08130 0.54 +0.3 1,788,062 + truD OKGIIK_08130 0.54 +1.1 1,788,063 - truD OKGIIK_08130 0.54 -0.7 1,788,063 - truD OKGIIK_08130 0.54 -1.3 1,788,063 - truD OKGIIK_08130 0.54 -0.3 1,788,063 - truD OKGIIK_08130 0.54 -1.3 1,788,063 - truD OKGIIK_08130 0.54 +0.8 1,788,063 - truD OKGIIK_08130 0.54 -0.5 1,788,063 - truD OKGIIK_08130 0.54 -1.5 1,788,063 - truD OKGIIK_08130 0.54 -0.3 1,788,063 - truD OKGIIK_08130 0.54 -0.9 1,788,063 - truD OKGIIK_08130 0.54 -1.8 1,788,317 + truD OKGIIK_08130 0.79 -1.2 1,788,318 - truD OKGIIK_08130 0.79 -0.9 1,788,437 + +0.4 1,788,437 + -0.5 1,788,438 - -2.4 1,788,438 - +1.3 1,788,438 - +0.7 1,788,516 - -0.1 1,788,516 - -0.1 1,788,516 - -1.5 1,788,516 - -0.2 1,788,555 - -0.3 1,788,555 - +0.4 1,788,625 + -2.1 1,788,625 + -0.2 1,788,625 + +0.3 1,788,625 + -1.8 1,788,625 + -0.8 1,788,625 + +1.3 1,788,625 + -2.1 1,788,625 + -0.9 1,788,625 + -0.1 1,788,625 + -0.8 1,788,625 + +0.1 1,788,625 + -0.9 1,788,625 + +1.1 1,788,625 + -0.9 1,788,625 + -0.6 1,788,625 + +0.4 1,788,625 + +0.1 1,788,625 + -1.3 1,788,625 + +0.9 1,788,625 + -0.5 1,788,659 + +1.1 1,788,659 + +0.5 1,788,659 + -0.9 1,788,830 + mgtE OKGIIK_08135 0.10 +1.1 1,788,830 + mgtE OKGIIK_08135 0.10 +0.1 1,788,830 + mgtE OKGIIK_08135 0.10 +0.4 1,788,830 + mgtE OKGIIK_08135 0.10 -0.4 1,788,831 - mgtE OKGIIK_08135 0.11 -0.9 1,788,831 - mgtE OKGIIK_08135 0.11 -0.5 1,788,831 - mgtE OKGIIK_08135 0.11 -1.5 1,788,831 - mgtE OKGIIK_08135 0.11 -1.9 1,788,831 - mgtE OKGIIK_08135 0.11 -1.1 1,788,831 - mgtE OKGIIK_08135 0.11 -0.3 1,788,831 - mgtE OKGIIK_08135 0.11 +0.8 1,788,831 - mgtE OKGIIK_08135 0.11 +1.4 1,788,831 - mgtE OKGIIK_08135 0.11 +1.1 1,789,296 - mgtE OKGIIK_08135 0.46 -0.9 1,789,322 + mgtE OKGIIK_08135 0.47 +1.7 1,789,322 + mgtE OKGIIK_08135 0.47 -0.4 1,789,322 + mgtE OKGIIK_08135 0.47 +1.4 1,789,322 + mgtE OKGIIK_08135 0.47 +1.1 1,789,322 + mgtE OKGIIK_08135 0.47 -0.1 1,789,323 - mgtE OKGIIK_08135 0.48 -1.5 1,789,323 - mgtE OKGIIK_08135 0.48 +1.3 1,789,323 - mgtE OKGIIK_08135 0.48 -1.3 1,789,323 - mgtE OKGIIK_08135 0.48 -0.9 1,789,499 + mgtE OKGIIK_08135 0.61 -1.8 1,789,499 + mgtE OKGIIK_08135 0.61 +0.1 1,789,499 + mgtE OKGIIK_08135 0.61 +0.8 1,789,500 - mgtE OKGIIK_08135 0.61 +0.8 1,789,583 + mgtE OKGIIK_08135 0.67 -0.9 1,789,583 + mgtE OKGIIK_08135 0.67 +0.8 1,789,584 - mgtE OKGIIK_08135 0.67 -0.5 1,789,584 - mgtE OKGIIK_08135 0.67 -2.1 1,789,584 - mgtE OKGIIK_08135 0.67 -0.8
Or see this region's nucleotide sequence