Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsP and OKGIIK_02240 are separated by 142 nucleotides OKGIIK_02240 and manX overlap by 8 nucleotides manX and rapZ are separated by 43 nucleotides rapZ and hprK are separated by 4 nucleotides
OKGIIK_02235: ptsP - phosphoenolpyruvate--protein phosphotransferase, at 525,065 to 526,807
ptsP
OKGIIK_02240: OKGIIK_02240 - Phosphocarrier protein HPr, at 526,950 to 527,219
_02240
OKGIIK_02245: manX - PTS fructose IIA subunit family protein, at 527,212 to 527,604
manX
OKGIIK_02250: rapZ - RNase adapter RapZ, at 527,648 to 528,550
rapZ
OKGIIK_02255: hprK - HPr(Ser) kinase/phosphatase, at 528,555 to 529,457
hprK
Position (kb) 527
528 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 526.233 kb on + strand, within ptsP at 526.233 kb on + strand, within ptsP at 526.233 kb on + strand, within ptsP at 526.434 kb on + strand, within ptsP at 526.434 kb on + strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.729 kb on - strand at 526.729 kb on - strand at 526.729 kb on - strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.952 kb on - strand at 527.121 kb on + strand, within OKGIIK_02240 at 527.121 kb on + strand, within OKGIIK_02240 at 527.121 kb on + strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.299 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.396 kb on + strand, within manX at 527.397 kb on - strand, within manX at 527.397 kb on - strand, within manX at 527.397 kb on - strand, within manX at 527.724 kb on + strand at 527.724 kb on + strand at 527.724 kb on + strand at 527.724 kb on + strand at 527.725 kb on - strand at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 528.033 kb on + strand, within rapZ at 528.169 kb on + strand, within rapZ at 528.170 kb on - strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.458 kb on + strand, within rapZ
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x remove 526,233 + ptsP OKGIIK_02235 0.67 -1.6 526,233 + ptsP OKGIIK_02235 0.67 +0.2 526,233 + ptsP OKGIIK_02235 0.67 +0.6 526,434 + ptsP OKGIIK_02235 0.79 +0.5 526,434 + ptsP OKGIIK_02235 0.79 +0.2 526,435 - ptsP OKGIIK_02235 0.79 -1.2 526,435 - ptsP OKGIIK_02235 0.79 +0.8 526,435 - ptsP OKGIIK_02235 0.79 -0.5 526,435 - ptsP OKGIIK_02235 0.79 -0.4 526,728 + -2.1 526,728 + -0.4 526,728 + +1.8 526,729 - +0.1 526,729 - +0.2 526,729 - -0.2 526,951 + -2.1 526,951 + -1.4 526,951 + -1.5 526,952 - +0.1 527,121 + OKGIIK_02240 0.63 -0.4 527,121 + OKGIIK_02240 0.63 +0.5 527,121 + OKGIIK_02240 0.63 -0.2 527,122 - OKGIIK_02240 0.64 +0.2 527,122 - OKGIIK_02240 0.64 -0.8 527,122 - OKGIIK_02240 0.64 +0.6 527,122 - OKGIIK_02240 0.64 -0.4 527,122 - OKGIIK_02240 0.64 -1.1 527,122 - OKGIIK_02240 0.64 -0.1 527,122 - OKGIIK_02240 0.64 -0.5 527,122 - OKGIIK_02240 0.64 -1.1 527,122 - OKGIIK_02240 0.64 -0.6 527,291 + manX OKGIIK_02245 0.20 -0.0 527,291 + manX OKGIIK_02245 0.20 -1.4 527,291 + manX OKGIIK_02245 0.20 -1.0 527,291 + manX OKGIIK_02245 0.20 -0.6 527,291 + manX OKGIIK_02245 0.20 -0.7 527,291 + manX OKGIIK_02245 0.20 +0.1 527,291 + manX OKGIIK_02245 0.20 -0.9 527,291 + manX OKGIIK_02245 0.20 +1.0 527,291 + manX OKGIIK_02245 0.20 -0.8 527,291 + manX OKGIIK_02245 0.20 -1.3 527,291 + manX OKGIIK_02245 0.20 +1.2 527,291 + manX OKGIIK_02245 0.20 -0.0 527,291 + manX OKGIIK_02245 0.20 -2.0 527,291 + manX OKGIIK_02245 0.20 +1.5 527,292 - manX OKGIIK_02245 0.20 -1.7 527,292 - manX OKGIIK_02245 0.20 +0.2 527,292 - manX OKGIIK_02245 0.20 -1.4 527,292 - manX OKGIIK_02245 0.20 -0.8 527,292 - manX OKGIIK_02245 0.20 -0.4 527,292 - manX OKGIIK_02245 0.20 -1.8 527,292 - manX OKGIIK_02245 0.20 -2.1 527,292 - manX OKGIIK_02245 0.20 +0.1 527,292 - manX OKGIIK_02245 0.20 -0.8 527,292 - manX OKGIIK_02245 0.20 -0.6 527,292 - manX OKGIIK_02245 0.20 -0.2 527,292 - manX OKGIIK_02245 0.20 -1.2 527,292 - manX OKGIIK_02245 0.20 +0.9 527,292 - manX OKGIIK_02245 0.20 -1.2 527,292 - manX OKGIIK_02245 0.20 -2.7 527,299 + manX OKGIIK_02245 0.22 -0.3 527,347 + manX OKGIIK_02245 0.34 -0.9 527,347 + manX OKGIIK_02245 0.34 -1.1 527,347 + manX OKGIIK_02245 0.34 -2.0 527,347 + manX OKGIIK_02245 0.34 -0.0 527,347 + manX OKGIIK_02245 0.34 -0.2 527,347 + manX OKGIIK_02245 0.34 -0.6 527,347 + manX OKGIIK_02245 0.34 +0.6 527,348 - manX OKGIIK_02245 0.35 -2.2 527,348 - manX OKGIIK_02245 0.35 -1.5 527,348 - manX OKGIIK_02245 0.35 -0.2 527,348 - manX OKGIIK_02245 0.35 +0.2 527,348 - manX OKGIIK_02245 0.35 -2.5 527,348 - manX OKGIIK_02245 0.35 -1.5 527,348 - manX OKGIIK_02245 0.35 -1.4 527,396 + manX OKGIIK_02245 0.47 -1.0 527,397 - manX OKGIIK_02245 0.47 -3.0 527,397 - manX OKGIIK_02245 0.47 +0.9 527,397 - manX OKGIIK_02245 0.47 +0.8 527,724 + -0.4 527,724 + -1.0 527,724 + -1.6 527,724 + -1.6 527,725 - -1.6 527,772 + rapZ OKGIIK_02250 0.14 -1.7 527,772 + rapZ OKGIIK_02250 0.14 -2.6 527,772 + rapZ OKGIIK_02250 0.14 -2.1 527,772 + rapZ OKGIIK_02250 0.14 -1.5 527,773 - rapZ OKGIIK_02250 0.14 -1.4 527,773 - rapZ OKGIIK_02250 0.14 -0.7 527,773 - rapZ OKGIIK_02250 0.14 -0.2 527,773 - rapZ OKGIIK_02250 0.14 -3.1 527,773 - rapZ OKGIIK_02250 0.14 +0.5 527,838 + rapZ OKGIIK_02250 0.21 -0.8 527,838 + rapZ OKGIIK_02250 0.21 -1.6 527,838 + rapZ OKGIIK_02250 0.21 +0.2 527,838 + rapZ OKGIIK_02250 0.21 +0.4 527,838 + rapZ OKGIIK_02250 0.21 -2.1 527,838 + rapZ OKGIIK_02250 0.21 -1.4 527,838 + rapZ OKGIIK_02250 0.21 -1.0 527,838 + rapZ OKGIIK_02250 0.21 -1.0 527,838 + rapZ OKGIIK_02250 0.21 -1.6 527,839 - rapZ OKGIIK_02250 0.21 +1.8 527,839 - rapZ OKGIIK_02250 0.21 -1.1 527,839 - rapZ OKGIIK_02250 0.21 +0.2 527,839 - rapZ OKGIIK_02250 0.21 -1.6 527,839 - rapZ OKGIIK_02250 0.21 -1.5 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -0.9 527,839 - rapZ OKGIIK_02250 0.21 -1.1 527,839 - rapZ OKGIIK_02250 0.21 -1.4 527,839 - rapZ OKGIIK_02250 0.21 -0.7 527,839 - rapZ OKGIIK_02250 0.21 -1.8 527,839 - rapZ OKGIIK_02250 0.21 -1.0 527,839 - rapZ OKGIIK_02250 0.21 -2.1 528,033 + rapZ OKGIIK_02250 0.43 -1.8 528,169 + rapZ OKGIIK_02250 0.58 -0.4 528,170 - rapZ OKGIIK_02250 0.58 -0.1 528,423 + rapZ OKGIIK_02250 0.86 -1.4 528,423 + rapZ OKGIIK_02250 0.86 -1.6 528,423 + rapZ OKGIIK_02250 0.86 -1.4 528,423 + rapZ OKGIIK_02250 0.86 -1.3 528,423 + rapZ OKGIIK_02250 0.86 -0.1 528,424 - rapZ OKGIIK_02250 0.86 -0.4 528,424 - rapZ OKGIIK_02250 0.86 +0.2 528,424 - rapZ OKGIIK_02250 0.86 -2.1 528,424 - rapZ OKGIIK_02250 0.86 -2.1 528,424 - rapZ OKGIIK_02250 0.86 -1.1 528,424 - rapZ OKGIIK_02250 0.86 -0.0 528,424 - rapZ OKGIIK_02250 0.86 -1.4 528,424 - rapZ OKGIIK_02250 0.86 -2.0 528,424 - rapZ OKGIIK_02250 0.86 -0.1 528,424 - rapZ OKGIIK_02250 0.86 +0.8 528,424 - rapZ OKGIIK_02250 0.86 +0.4 528,424 - rapZ OKGIIK_02250 0.86 -2.1 528,458 + rapZ OKGIIK_02250 0.90 -0.7
Or see this region's nucleotide sequence