Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27585

Experiment: TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclsA and cls are separated by 34 nucleotidescls and oppF are separated by 52 nucleotidesoppF and oppD overlap by 4 nucleotides NIAGMN_27575: clsA - cardiolipin synthase ClsA, at 5,342,301 to 5,343,761 clsA NIAGMN_27580: cls - cardiolipin synthase, at 5,343,796 to 5,344,125 cls NIAGMN_27585: oppF - murein tripeptide/oligopeptide ABC transporter ATP-binding protein OppF, at 5,344,178 to 5,345,182 oppF NIAGMN_27590: oppD - murein tripeptide/oligopeptide ABC transporter ATP-binding protein OppD, at 5,345,179 to 5,346,192 oppD Position (kb) 5344 5345 5346Strain fitness (log2 ratio) -1 0 1at 5343.188 kb on + strand, within clsAat 5343.189 kb on - strand, within clsAat 5343.660 kb on - strandat 5344.405 kb on - strand, within oppFat 5344.437 kb on + strand, within oppFat 5344.438 kb on - strand, within oppFat 5344.468 kb on - strand, within oppFat 5344.468 kb on - strand, within oppFat 5344.530 kb on - strand, within oppFat 5344.796 kb on - strand, within oppFat 5345.340 kb on + strand, within oppDat 5345.403 kb on - strand, within oppDat 5345.631 kb on + strand, within oppDat 5345.641 kb on + strand, within oppDat 5345.642 kb on - strand, within oppD

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Per-strain Table

Position Strand Gene LocusTag Fraction TP9
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5,343,188 + clsA NIAGMN_27575 0.61 +0.5
5,343,189 - clsA NIAGMN_27575 0.61 +0.1
5,343,660 - -1.5
5,344,405 - oppF NIAGMN_27585 0.23 +0.9
5,344,437 + oppF NIAGMN_27585 0.26 +1.0
5,344,438 - oppF NIAGMN_27585 0.26 +0.7
5,344,468 - oppF NIAGMN_27585 0.29 +0.9
5,344,468 - oppF NIAGMN_27585 0.29 -0.8
5,344,530 - oppF NIAGMN_27585 0.35 -0.9
5,344,796 - oppF NIAGMN_27585 0.61 +1.3
5,345,340 + oppD NIAGMN_27590 0.16 -0.8
5,345,403 - oppD NIAGMN_27590 0.22 +0.5
5,345,631 + oppD NIAGMN_27590 0.45 -0.5
5,345,641 + oppD NIAGMN_27590 0.46 +0.9
5,345,642 - oppD NIAGMN_27590 0.46 -0.8

Or see this region's nucleotide sequence