Strain Fitness in Escherichia coli ECRC102 around NIAGMN_25295

Experiment: TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttam and lsrG are separated by 56 nucleotideslsrG and lsrF are separated by 23 nucleotideslsrF and lsrB are separated by 26 nucleotides NIAGMN_25285: tam - trans-aconitate 2-methyltransferase, at 4,913,875 to 4,914,633 tam NIAGMN_25290: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 4,914,690 to 4,914,980 lsrG NIAGMN_25295: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 4,915,004 to 4,915,879 lsrF NIAGMN_25300: lsrB - autoinducer 2 ABC transporter substrate-binding protein LsrB, at 4,915,906 to 4,916,928 lsrB Position (kb) 4915 4916Strain fitness (log2 ratio) -2 -1 0 1 2at 4914.278 kb on - strand, within tamat 4914.611 kb on - strandat 4914.769 kb on + strand, within lsrGat 4914.814 kb on + strand, within lsrGat 4914.892 kb on - strand, within lsrGat 4914.981 kb on - strandat 4915.264 kb on - strand, within lsrFat 4915.626 kb on - strand, within lsrFat 4915.626 kb on - strand, within lsrFat 4915.661 kb on + strand, within lsrFat 4915.662 kb on - strand, within lsrFat 4915.858 kb on + strandat 4915.898 kb on - strandat 4915.943 kb on - strandat 4915.964 kb on - strandat 4915.964 kb on - strandat 4915.964 kb on - strandat 4916.168 kb on + strand, within lsrBat 4916.335 kb on - strand, within lsrBat 4916.347 kb on - strand, within lsrBat 4916.695 kb on - strand, within lsrBat 4916.842 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP9
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4,914,278 - tam NIAGMN_25285 0.53 +0.7
4,914,611 - -0.0
4,914,769 + lsrG NIAGMN_25290 0.27 +0.4
4,914,814 + lsrG NIAGMN_25290 0.43 -0.2
4,914,892 - lsrG NIAGMN_25290 0.69 +0.6
4,914,981 - +0.2
4,915,264 - lsrF NIAGMN_25295 0.30 +0.6
4,915,626 - lsrF NIAGMN_25295 0.71 -1.8
4,915,626 - lsrF NIAGMN_25295 0.71 -1.4
4,915,661 + lsrF NIAGMN_25295 0.75 -0.3
4,915,662 - lsrF NIAGMN_25295 0.75 +0.6
4,915,858 + -2.3
4,915,898 - +1.1
4,915,943 - -0.8
4,915,964 - -1.4
4,915,964 - -1.2
4,915,964 - +2.6
4,916,168 + lsrB NIAGMN_25300 0.26 +1.1
4,916,335 - lsrB NIAGMN_25300 0.42 +0.3
4,916,347 - lsrB NIAGMN_25300 0.43 -0.4
4,916,695 - lsrB NIAGMN_25300 0.77 +0.6
4,916,842 - -1.2

Or see this region's nucleotide sequence