Strain Fitness in Escherichia coli ECRC102 around NIAGMN_24385

Experiment: TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlolE and nagK are separated by 28 nucleotidesnagK and cobB are separated by 15 nucleotidescobB and potD are separated by 155 nucleotides NIAGMN_24380: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 4,770,199 to 4,771,437 lolE NIAGMN_24385: nagK - N-acetylglucosamine kinase, at 4,771,466 to 4,772,377 nagK NIAGMN_24390: cobB - NAD-dependent protein deacylase, at 4,772,393 to 4,773,214 cobB NIAGMN_24395: potD - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 4,773,370 to 4,774,416 potD Position (kb) 4771 4772 4773Strain fitness (log2 ratio) -2 -1 0 1 2at 4771.444 kb on + strandat 4771.444 kb on + strandat 4771.445 kb on - strandat 4771.449 kb on + strandat 4771.449 kb on + strandat 4771.450 kb on - strandat 4771.469 kb on + strandat 4771.571 kb on + strand, within nagKat 4771.633 kb on + strand, within nagKat 4771.764 kb on + strand, within nagKat 4772.067 kb on - strand, within nagKat 4772.070 kb on - strand, within nagKat 4772.108 kb on + strand, within nagKat 4772.292 kb on + strandat 4772.361 kb on + strandat 4772.785 kb on - strand, within cobBat 4772.908 kb on + strand, within cobBat 4772.953 kb on - strand, within cobBat 4772.993 kb on + strand, within cobBat 4773.210 kb on + strandat 4773.212 kb on + strandat 4773.372 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP9
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4,771,444 + -1.1
4,771,444 + -1.6
4,771,445 - -0.1
4,771,449 + +1.7
4,771,449 + -1.2
4,771,450 - -2.1
4,771,469 + +1.7
4,771,571 + nagK NIAGMN_24385 0.12 +0.2
4,771,633 + nagK NIAGMN_24385 0.18 +0.7
4,771,764 + nagK NIAGMN_24385 0.33 -0.6
4,772,067 - nagK NIAGMN_24385 0.66 -0.3
4,772,070 - nagK NIAGMN_24385 0.66 +1.1
4,772,108 + nagK NIAGMN_24385 0.70 +2.3
4,772,292 + +0.7
4,772,361 + +1.0
4,772,785 - cobB NIAGMN_24390 0.48 +1.2
4,772,908 + cobB NIAGMN_24390 0.63 -0.3
4,772,953 - cobB NIAGMN_24390 0.68 +1.1
4,772,993 + cobB NIAGMN_24390 0.73 +0.3
4,773,210 + -0.7
4,773,212 + +0.8
4,773,372 - -1.7

Or see this region's nucleotide sequence