Experiment: TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dgcP and yoaJ are separated by 310 nucleotides yoaJ and yeaQ are separated by 58 nucleotides yeaQ and yoaG are separated by 146 nucleotides yoaG and yeaR are separated by 3 nucleotides yeaR and leuE are separated by 172 nucleotides
NIAGMN_01405: dgcP - Diguanylate cyclase DgcP, at 254,902 to 255,750
dgcP
NIAGMN_01410: yoaJ - protein YoaJ, at 256,061 to 256,135
yoaJ
NIAGMN_01415: yeaQ - UPF0410 protein YeaQ, at 256,194 to 256,442
yeaQ
NIAGMN_01420: yoaG - Protein YoaG, at 256,589 to 256,771
yoaG
NIAGMN_01425: yeaR - Uncharacterized protein YeaR, at 256,775 to 257,134
yeaR
NIAGMN_01430: leuE - leucine efflux protein LeuE, at 257,307 to 257,945
leuE
Position (kb) 256
257 Strain fitness (log2 ratio) -2
-1
0
1
2 at 255.358 kb on + strand, within dgcP at 255.360 kb on + strand, within dgcP at 255.803 kb on + strand at 255.933 kb on - strand at 255.988 kb on + strand at 255.989 kb on - strand at 256.222 kb on - strand, within yeaQ at 256.497 kb on - strand at 256.756 kb on - strand at 256.857 kb on + strand, within yeaR at 256.862 kb on - strand, within yeaR at 257.245 kb on + strand at 257.271 kb on + strand at 257.401 kb on - strand, within leuE at 257.401 kb on - strand, within leuE at 257.401 kb on - strand, within leuE at 257.401 kb on - strand, within leuE at 257.430 kb on - strand, within leuE
Per-strain Table
Position Strand Gene LocusTag Fraction TP9 remove 255,358 + dgcP NIAGMN_01405 0.54 -0.2 255,360 + dgcP NIAGMN_01405 0.54 -0.5 255,803 + -0.2 255,933 - +0.1 255,988 + -1.9 255,989 - -0.1 256,222 - yeaQ NIAGMN_01415 0.11 +1.0 256,497 - +1.6 256,756 - -1.0 256,857 + yeaR NIAGMN_01425 0.23 +0.6 256,862 - yeaR NIAGMN_01425 0.24 -0.8 257,245 + -0.4 257,271 + +1.2 257,401 - leuE NIAGMN_01430 0.15 +0.6 257,401 - leuE NIAGMN_01430 0.15 +0.6 257,401 - leuE NIAGMN_01430 0.15 +2.6 257,401 - leuE NIAGMN_01430 0.15 -1.0 257,430 - leuE NIAGMN_01430 0.19 +0.4
Or see this region's nucleotide sequence