Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00635

Experiment: TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnemA and NIAGMN_00635 are separated by 80 nucleotidesNIAGMN_00635 and rnt are separated by 102 nucleotidesrnt and lhr are separated by 92 nucleotides NIAGMN_00630: nemA - N-ethylmaleimide reductase, at 101,956 to 103,053 nemA NIAGMN_00635: NIAGMN_00635 - Lactoylglutathione lyase, at 103,134 to 103,541 _00635 NIAGMN_00640: rnt - ribonuclease T, at 103,644 to 104,291 rnt NIAGMN_00645: lhr - ATP-dependent helicase, at 104,384 to 109,000 lhr Position (kb) 103 104Strain fitness (log2 ratio) -1 0 1 2at 102.515 kb on - strand, within nemAat 102.672 kb on + strand, within nemAat 102.721 kb on + strand, within nemAat 102.765 kb on + strand, within nemAat 102.765 kb on + strand, within nemAat 102.766 kb on - strand, within nemAat 102.799 kb on + strand, within nemAat 102.865 kb on + strand, within nemAat 102.984 kb on + strandat 103.009 kb on + strandat 103.030 kb on + strandat 103.149 kb on - strandat 103.224 kb on - strand, within NIAGMN_00635at 103.308 kb on + strand, within NIAGMN_00635at 103.308 kb on + strand, within NIAGMN_00635at 103.347 kb on + strand, within NIAGMN_00635at 103.364 kb on + strand, within NIAGMN_00635at 103.373 kb on + strand, within NIAGMN_00635at 104.332 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP9
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102,515 - nemA NIAGMN_00630 0.51 -0.5
102,672 + nemA NIAGMN_00630 0.65 -0.4
102,721 + nemA NIAGMN_00630 0.70 -1.0
102,765 + nemA NIAGMN_00630 0.74 -0.1
102,765 + nemA NIAGMN_00630 0.74 -0.4
102,766 - nemA NIAGMN_00630 0.74 -0.4
102,799 + nemA NIAGMN_00630 0.77 +0.2
102,865 + nemA NIAGMN_00630 0.83 +1.0
102,984 + +0.9
103,009 + +2.6
103,030 + -0.0
103,149 - +0.2
103,224 - NIAGMN_00635 0.22 +0.1
103,308 + NIAGMN_00635 0.43 +0.6
103,308 + NIAGMN_00635 0.43 +1.6
103,347 + NIAGMN_00635 0.52 +1.3
103,364 + NIAGMN_00635 0.56 -0.1
103,373 + NIAGMN_00635 0.59 +0.6
104,332 - -1.2

Or see this region's nucleotide sequence