Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF4860

Experiment: Ying_all64 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4859 and GFF4860 are separated by 50 nucleotidesGFF4860 and GFF4861 are separated by 53 nucleotidesGFF4861 and GFF4862 are separated by 91 nucleotides GFF4859 - GTP-binding protein EngA, at 1,865,346 to 1,866,680 GFF4859 GFF4860 - Outer membrane protein YfgL, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and NlpB), at 1,866,731 to 1,867,837 GFF4860 GFF4861 - Mlr7403 protein, at 1,867,891 to 1,868,556 GFF4861 GFF4862 - Histidyl-tRNA synthetase (EC 6.1.1.21), at 1,868,648 to 1,869,952 GFF4862 Position (kb) 1866 1867 1868Strain fitness (log2 ratio) -2 -1 0 1at 1866.733 kb on - strandat 1866.733 kb on - strandat 1868.003 kb on + strand, within GFF4861at 1868.003 kb on + strand, within GFF4861at 1868.177 kb on + strand, within GFF4861at 1868.177 kb on + strand, within GFF4861at 1868.177 kb on + strand, within GFF4861at 1868.177 kb on + strand, within GFF4861at 1868.178 kb on - strand, within GFF4861at 1868.178 kb on - strand, within GFF4861at 1868.178 kb on - strand, within GFF4861at 1868.178 kb on - strand, within GFF4861at 1868.420 kb on + strand, within GFF4861at 1868.606 kb on + strandat 1868.606 kb on + strandat 1868.607 kb on - strandat 1868.607 kb on - strandat 1868.607 kb on - strandat 1868.607 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2
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1,866,733 - +0.7
1,866,733 - -0.5
1,868,003 + GFF4861 0.17 +0.7
1,868,003 + GFF4861 0.17 -1.3
1,868,177 + GFF4861 0.43 -1.5
1,868,177 + GFF4861 0.43 -0.5
1,868,177 + GFF4861 0.43 -1.9
1,868,177 + GFF4861 0.43 -0.8
1,868,178 - GFF4861 0.43 -1.6
1,868,178 - GFF4861 0.43 -2.7
1,868,178 - GFF4861 0.43 -1.8
1,868,178 - GFF4861 0.43 -1.5
1,868,420 + GFF4861 0.79 +0.1
1,868,606 + -0.0
1,868,606 + +1.2
1,868,607 - +0.9
1,868,607 - +0.0
1,868,607 - -0.2
1,868,607 - -1.3

Or see this region's nucleotide sequence