Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF43

Experiment: Ying_all64 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF42 and GFF43 are separated by 62 nucleotidesGFF43 and GFF44 overlap by 4 nucleotidesGFF44 and GFF45 overlap by 4 nucleotides GFF42 - glycosyl transferase group 1, at 42,240 to 43,385 GFF42 GFF43 - Glycosyl transferase, group 1, at 43,448 to 44,686 GFF43 GFF44 - glycosyl transferase, family 2, at 44,683 to 45,531 GFF44 GFF45 - Colanic acid biosynthesis acetyltransferase WcaF (EC 2.3.1.-), at 45,528 to 46,088 GFF45 Position (kb) 43 44 45Strain fitness (log2 ratio) -1 0 1at 42.762 kb on + strand, within GFF42at 43.037 kb on - strand, within GFF42at 43.037 kb on - strand, within GFF42at 43.392 kb on - strandat 44.559 kb on + strand, within GFF43at 44.560 kb on - strand, within GFF43at 44.628 kb on + strandat 44.629 kb on - strandat 44.760 kb on - strandat 44.831 kb on + strand, within GFF44at 44.831 kb on + strand, within GFF44at 44.985 kb on - strand, within GFF44at 45.104 kb on + strand, within GFF44at 45.137 kb on + strand, within GFF44at 45.138 kb on - strand, within GFF44at 45.138 kb on - strand, within GFF44

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2
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42,762 + GFF42 0.46 -0.9
43,037 - GFF42 0.70 +0.9
43,037 - GFF42 0.70 -0.3
43,392 - +0.0
44,559 + GFF43 0.90 -0.3
44,560 - GFF43 0.90 -0.1
44,628 + +1.6
44,629 - -1.5
44,760 - +1.6
44,831 + GFF44 0.17 +0.1
44,831 + GFF44 0.17 +0.1
44,985 - GFF44 0.36 +0.2
45,104 + GFF44 0.50 +0.3
45,137 + GFF44 0.53 +1.3
45,138 - GFF44 0.54 +0.4
45,138 - GFF44 0.54 -0.0

Or see this region's nucleotide sequence