Experiment: Ying_all64 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3052 and GFF3053 are separated by 28 nucleotides GFF3053 and GFF3054 are separated by 19 nucleotides GFF3054 and GFF3055 overlap by 1 nucleotides
GFF3052 - Tricarboxylate transport protein TctC, at 131,137 to 132,111
GFF3052
GFF3053 - Quinone oxidoreductase (EC 1.6.5.5), at 132,140 to 133,129
GFF3053
GFF3054 - Enoyl-CoA hydratase (EC 4.2.1.17), at 133,149 to 133,958
GFF3054
GFF3055 - L-carnitine dehydratase/bile acid-inducible protein F, at 133,958 to 135,148
GFF3055
Position (kb)
132
133
134 Strain fitness (log2 ratio)
-1
0
1 at 131.375 kb on + strand, within GFF3052 at 131.376 kb on - strand, within GFF3052 at 131.376 kb on - strand, within GFF3052 at 131.376 kb on - strand, within GFF3052 at 131.648 kb on + strand, within GFF3052 at 131.649 kb on - strand, within GFF3052 at 131.783 kb on + strand, within GFF3052 at 131.784 kb on - strand, within GFF3052 at 131.784 kb on - strand, within GFF3052 at 131.849 kb on + strand, within GFF3052 at 131.850 kb on - strand, within GFF3052 at 131.976 kb on - strand, within GFF3052 at 132.420 kb on + strand, within GFF3053 at 132.421 kb on - strand, within GFF3053 at 133.510 kb on + strand, within GFF3054 at 133.613 kb on - strand, within GFF3054 at 133.943 kb on - strand at 133.962 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2 remove 131,375 + GFF3052 0.24 -0.2 131,376 - GFF3052 0.25 +0.6 131,376 - GFF3052 0.25 +0.4 131,376 - GFF3052 0.25 +0.5 131,648 + GFF3052 0.52 +0.5 131,649 - GFF3052 0.53 -0.4 131,783 + GFF3052 0.66 -1.4 131,784 - GFF3052 0.66 +0.1 131,784 - GFF3052 0.66 -1.4 131,849 + GFF3052 0.73 -0.9 131,850 - GFF3052 0.73 -0.2 131,976 - GFF3052 0.86 -0.4 132,420 + GFF3053 0.28 -0.9 132,421 - GFF3053 0.28 -0.3 133,510 + GFF3054 0.45 -0.6 133,613 - GFF3054 0.57 -0.8 133,943 - -0.8 133,962 + +0.8
Or see this region's nucleotide sequence