Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF299

Experiment: Ying_all64 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF297 and GFF298 are separated by 24 nucleotidesGFF298 and GFF299 are separated by 22 nucleotidesGFF299 and GFF300 are separated by 19 nucleotides GFF297 - Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17), at 310,241 to 311,593 GFF297 GFF298 - DedD protein, at 311,618 to 312,352 GFF298 GFF299 - Colicin V production protein, at 312,375 to 312,860 GFF299 GFF300 - Amidophosphoribosyltransferase (EC 2.4.2.14), at 312,880 to 314,385 GFF300 Position (kb) 312 313Strain fitness (log2 ratio) -2 -1 0 1at 311.544 kb on + strandat 311.544 kb on + strandat 311.545 kb on - strandat 311.599 kb on + strandat 312.215 kb on + strand, within GFF298at 312.215 kb on + strand, within GFF298at 312.215 kb on + strand, within GFF298at 312.215 kb on + strand, within GFF298at 312.216 kb on - strand, within GFF298at 312.216 kb on - strand, within GFF298at 312.216 kb on - strand, within GFF298at 312.450 kb on + strand, within GFF299at 312.450 kb on + strand, within GFF299at 312.450 kb on + strand, within GFF299at 312.450 kb on + strand, within GFF299at 312.451 kb on - strand, within GFF299at 312.451 kb on - strand, within GFF299at 312.451 kb on - strand, within GFF299at 312.451 kb on - strand, within GFF299at 312.451 kb on - strand, within GFF299at 312.849 kb on + strandat 312.850 kb on - strandat 312.850 kb on - strandat 312.850 kb on - strandat 312.850 kb on - strandat 312.851 kb on + strandat 312.851 kb on + strandat 312.851 kb on + strandat 312.851 kb on + strandat 312.851 kb on + strandat 312.852 kb on - strandat 312.852 kb on - strandat 312.852 kb on - strandat 312.852 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2
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311,544 + +0.5
311,544 + -0.1
311,545 - -0.2
311,599 + -0.7
312,215 + GFF298 0.81 +1.0
312,215 + GFF298 0.81 -1.0
312,215 + GFF298 0.81 -1.9
312,215 + GFF298 0.81 -0.2
312,216 - GFF298 0.81 +1.3
312,216 - GFF298 0.81 +0.0
312,216 - GFF298 0.81 -0.2
312,450 + GFF299 0.15 -0.6
312,450 + GFF299 0.15 -0.1
312,450 + GFF299 0.15 +0.1
312,450 + GFF299 0.15 -1.1
312,451 - GFF299 0.16 +1.7
312,451 - GFF299 0.16 -0.2
312,451 - GFF299 0.16 +0.4
312,451 - GFF299 0.16 +1.7
312,451 - GFF299 0.16 +0.3
312,849 + -0.6
312,850 - +0.3
312,850 - -0.7
312,850 - +0.7
312,850 - +0.0
312,851 + +0.7
312,851 + +0.9
312,851 + +0.2
312,851 + -1.2
312,851 + -0.5
312,852 - +1.0
312,852 - -0.8
312,852 - +0.8
312,852 - -0.2

Or see this region's nucleotide sequence