Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dAP2 and traX are separated by 58 nucleotides traX and NIAGMN_28750 are separated by 19 nucleotides NIAGMN_28750 and insE are separated by 188 nucleotides insE and NIAGMN_28760 overlap by 74 nucleotides NIAGMN_28760 and toxB are separated by 91 nucleotides
NIAGMN_28740: dAP2 - Uncharacterized protein in traX-finO intergenic region, at 49,754 to 50,614
dAP2
NIAGMN_28745: traX - conjugal transfer pilus acetylase TraX, at 50,673 to 51,419
traX
NIAGMN_28750: NIAGMN_28750 - TraI domain-containing protein, at 51,439 to 52,695
_28750
NIAGMN_28755: insE - Transposase InsE and inactivated derivatives, at 52,884 to 53,387
insE
NIAGMN_28760: NIAGMN_28760 - Transposase Tra5, at 53,314 to 53,586
_28760
NIAGMN_28765: toxB - toxin B, at 53,678 to 63,187
toxB
Position (kb) 51
52
53 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 50.525 kb on + strand, within dAP2 at 50.525 kb on + strand, within dAP2 at 50.526 kb on - strand, within dAP2 at 50.526 kb on - strand, within dAP2 at 50.526 kb on - strand, within dAP2 at 50.530 kb on + strand at 50.530 kb on + strand at 50.531 kb on - strand at 50.531 kb on - strand at 50.547 kb on - strand at 50.547 kb on - strand at 50.547 kb on - strand at 50.547 kb on - strand at 50.549 kb on - strand at 50.549 kb on - strand at 50.582 kb on - strand at 50.582 kb on - strand at 50.606 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.619 kb on - strand at 50.628 kb on + strand at 50.628 kb on + strand at 50.628 kb on + strand at 50.628 kb on + strand at 50.629 kb on - strand at 50.629 kb on - strand at 50.629 kb on - strand at 50.629 kb on - strand at 50.629 kb on - strand at 50.629 kb on - strand at 50.629 kb on - strand at 50.638 kb on - strand at 50.640 kb on + strand at 50.640 kb on + strand at 50.643 kb on + strand at 50.663 kb on - strand at 50.663 kb on - strand at 50.663 kb on - strand at 50.713 kb on + strand at 50.713 kb on + strand at 50.713 kb on + strand at 50.713 kb on + strand at 50.714 kb on - strand at 50.714 kb on - strand at 50.714 kb on - strand at 50.714 kb on - strand at 50.714 kb on - strand at 50.714 kb on - strand at 50.811 kb on + strand, within traX at 50.811 kb on + strand, within traX at 50.812 kb on - strand, within traX at 50.812 kb on - strand, within traX at 50.812 kb on - strand, within traX at 50.812 kb on - strand, within traX at 50.840 kb on + strand, within traX at 50.840 kb on + strand, within traX at 50.840 kb on + strand, within traX at 50.840 kb on + strand, within traX at 50.841 kb on - strand, within traX at 50.841 kb on - strand, within traX at 50.841 kb on - strand, within traX at 50.954 kb on - strand, within traX at 51.008 kb on + strand, within traX at 51.008 kb on + strand, within traX at 51.009 kb on - strand, within traX at 51.009 kb on - strand, within traX at 51.074 kb on + strand, within traX at 51.074 kb on + strand, within traX at 51.085 kb on + strand, within traX at 51.086 kb on - strand, within traX at 51.106 kb on + strand, within traX at 51.106 kb on + strand, within traX at 51.106 kb on + strand, within traX at 51.106 kb on + strand, within traX at 51.106 kb on + strand, within traX at 51.107 kb on - strand, within traX at 51.107 kb on - strand, within traX at 51.107 kb on - strand, within traX at 51.107 kb on - strand, within traX at 51.125 kb on + strand, within traX at 51.125 kb on + strand, within traX at 51.126 kb on - strand, within traX at 51.126 kb on - strand, within traX at 51.167 kb on + strand, within traX at 51.167 kb on + strand, within traX at 51.168 kb on - strand, within traX at 51.285 kb on + strand, within traX at 51.286 kb on - strand, within traX at 51.389 kb on + strand at 51.389 kb on + strand at 51.389 kb on + strand at 51.389 kb on + strand at 51.389 kb on + strand at 51.390 kb on - strand at 51.390 kb on - strand at 51.407 kb on + strand at 51.437 kb on + strand at 51.437 kb on + strand at 51.437 kb on + strand at 51.438 kb on - strand at 51.438 kb on - strand at 51.483 kb on + strand at 51.574 kb on + strand, within NIAGMN_28750 at 51.574 kb on + strand, within NIAGMN_28750 at 51.575 kb on - strand, within NIAGMN_28750 at 51.575 kb on - strand, within NIAGMN_28750 at 51.575 kb on - strand, within NIAGMN_28750 at 51.699 kb on + strand, within NIAGMN_28750 at 51.770 kb on - strand, within NIAGMN_28750 at 51.918 kb on + strand, within NIAGMN_28750 at 51.919 kb on - strand, within NIAGMN_28750 at 51.919 kb on - strand, within NIAGMN_28750 at 51.948 kb on + strand, within NIAGMN_28750 at 51.948 kb on + strand, within NIAGMN_28750 at 51.949 kb on - strand, within NIAGMN_28750 at 51.949 kb on - strand, within NIAGMN_28750 at 51.949 kb on - strand, within NIAGMN_28750 at 51.949 kb on - strand, within NIAGMN_28750 at 51.949 kb on - strand, within NIAGMN_28750 at 51.951 kb on + strand, within NIAGMN_28750 at 51.952 kb on - strand, within NIAGMN_28750 at 52.133 kb on + strand, within NIAGMN_28750 at 52.133 kb on + strand, within NIAGMN_28750 at 52.133 kb on + strand, within NIAGMN_28750 at 52.133 kb on + strand, within NIAGMN_28750 at 52.133 kb on + strand, within NIAGMN_28750 at 52.133 kb on + strand, within NIAGMN_28750 at 52.134 kb on - strand, within NIAGMN_28750 at 52.134 kb on - strand, within NIAGMN_28750 at 52.134 kb on - strand, within NIAGMN_28750 at 52.134 kb on - strand, within NIAGMN_28750 at 52.134 kb on - strand, within NIAGMN_28750 at 52.134 kb on - strand, within NIAGMN_28750 at 52.134 kb on - strand, within NIAGMN_28750 at 52.134 kb on - strand, within NIAGMN_28750 at 52.145 kb on + strand, within NIAGMN_28750 at 52.145 kb on + strand, within NIAGMN_28750 at 52.146 kb on - strand, within NIAGMN_28750 at 52.146 kb on - strand, within NIAGMN_28750 at 52.146 kb on - strand, within NIAGMN_28750 at 52.149 kb on + strand, within NIAGMN_28750 at 52.149 kb on + strand, within NIAGMN_28750 at 52.149 kb on + strand, within NIAGMN_28750 at 52.150 kb on - strand, within NIAGMN_28750 at 52.164 kb on + strand, within NIAGMN_28750 at 52.164 kb on + strand, within NIAGMN_28750 at 52.164 kb on + strand, within NIAGMN_28750 at 52.373 kb on + strand, within NIAGMN_28750 at 52.374 kb on - strand, within NIAGMN_28750 at 52.374 kb on - strand, within NIAGMN_28750 at 52.374 kb on - strand, within NIAGMN_28750 at 52.396 kb on - strand, within NIAGMN_28750 at 52.396 kb on - strand, within NIAGMN_28750 at 52.412 kb on + strand, within NIAGMN_28750 at 52.412 kb on + strand, within NIAGMN_28750 at 52.412 kb on + strand, within NIAGMN_28750 at 52.413 kb on - strand, within NIAGMN_28750 at 52.413 kb on - strand, within NIAGMN_28750 at 52.456 kb on - strand, within NIAGMN_28750 at 52.518 kb on - strand, within NIAGMN_28750 at 52.518 kb on - strand, within NIAGMN_28750 at 52.518 kb on - strand, within NIAGMN_28750 at 52.530 kb on + strand, within NIAGMN_28750 at 52.530 kb on + strand, within NIAGMN_28750 at 52.530 kb on + strand, within NIAGMN_28750 at 52.530 kb on + strand, within NIAGMN_28750 at 52.531 kb on - strand, within NIAGMN_28750 at 52.531 kb on - strand, within NIAGMN_28750 at 52.643 kb on + strand at 52.643 kb on + strand at 52.643 kb on + strand at 52.643 kb on + strand at 52.643 kb on + strand at 52.644 kb on - strand at 52.644 kb on - strand at 52.644 kb on - strand at 52.767 kb on + strand at 52.789 kb on + strand at 52.813 kb on - strand at 53.152 kb on + strand, within insE at 53.185 kb on + strand, within insE at 53.186 kb on - strand, within insE at 53.186 kb on - strand, within insE at 53.186 kb on - strand, within insE at 53.186 kb on - strand, within insE at 53.186 kb on - strand, within insE at 53.269 kb on + strand, within insE at 53.270 kb on - strand, within insE at 53.308 kb on - strand, within insE at 53.382 kb on - strand at 53.382 kb on - strand at 53.382 kb on - strand at 53.382 kb on - strand at 53.382 kb on - strand at 53.382 kb on - strand at 53.382 kb on - strand at 53.382 kb on - strand at 53.382 kb on - strand at 53.382 kb on - strand at 53.603 kb on + strand at 53.604 kb on - strand at 53.604 kb on - strand at 53.604 kb on - strand at 53.604 kb on - strand at 53.604 kb on - strand at 53.604 kb on - strand at 53.640 kb on - strand at 53.645 kb on + strand at 53.646 kb on - strand at 53.679 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 50,525 + dAP2 NIAGMN_28740 0.90 +0.4 50,525 + dAP2 NIAGMN_28740 0.90 -0.8 50,526 - dAP2 NIAGMN_28740 0.90 +0.3 50,526 - dAP2 NIAGMN_28740 0.90 -0.0 50,526 - dAP2 NIAGMN_28740 0.90 -0.2 50,530 + -0.3 50,530 + +1.5 50,531 - -0.5 50,531 - +0.2 50,547 - +0.4 50,547 - -0.3 50,547 - +0.4 50,547 - -0.5 50,549 - +0.8 50,549 - -0.2 50,582 - -0.4 50,582 - -1.0 50,606 - -0.3 50,615 - -0.8 50,615 - -0.4 50,619 - -0.2 50,628 + -1.8 50,628 + -0.8 50,628 + -0.8 50,628 + +0.9 50,629 - +0.3 50,629 - -0.4 50,629 - -0.7 50,629 - -0.4 50,629 - -0.4 50,629 - -0.7 50,629 - -0.5 50,638 - -0.9 50,640 + -2.6 50,640 + +0.4 50,643 + +0.3 50,663 - -0.5 50,663 - +0.5 50,663 - -0.9 50,713 + +0.4 50,713 + -0.1 50,713 + -0.6 50,713 + -0.2 50,714 - +0.3 50,714 - -0.6 50,714 - +1.2 50,714 - -1.3 50,714 - -1.1 50,714 - +0.3 50,811 + traX NIAGMN_28745 0.18 -0.3 50,811 + traX NIAGMN_28745 0.18 +0.2 50,812 - traX NIAGMN_28745 0.19 -0.8 50,812 - traX NIAGMN_28745 0.19 -0.6 50,812 - traX NIAGMN_28745 0.19 +1.9 50,812 - traX NIAGMN_28745 0.19 -0.5 50,840 + traX NIAGMN_28745 0.22 -0.1 50,840 + traX NIAGMN_28745 0.22 -1.0 50,840 + traX NIAGMN_28745 0.22 -0.7 50,840 + traX NIAGMN_28745 0.22 -0.1 50,841 - traX NIAGMN_28745 0.22 -0.6 50,841 - traX NIAGMN_28745 0.22 +0.5 50,841 - traX NIAGMN_28745 0.22 -2.4 50,954 - traX NIAGMN_28745 0.38 -0.3 51,008 + traX NIAGMN_28745 0.45 -0.9 51,008 + traX NIAGMN_28745 0.45 -2.5 51,009 - traX NIAGMN_28745 0.45 +0.5 51,009 - traX NIAGMN_28745 0.45 -1.5 51,074 + traX NIAGMN_28745 0.54 -0.3 51,074 + traX NIAGMN_28745 0.54 -2.2 51,085 + traX NIAGMN_28745 0.55 +1.3 51,086 - traX NIAGMN_28745 0.55 -1.6 51,106 + traX NIAGMN_28745 0.58 -0.7 51,106 + traX NIAGMN_28745 0.58 +0.8 51,106 + traX NIAGMN_28745 0.58 +0.6 51,106 + traX NIAGMN_28745 0.58 -1.1 51,106 + traX NIAGMN_28745 0.58 -0.1 51,107 - traX NIAGMN_28745 0.58 -0.9 51,107 - traX NIAGMN_28745 0.58 +0.8 51,107 - traX NIAGMN_28745 0.58 -0.2 51,107 - traX NIAGMN_28745 0.58 -1.0 51,125 + traX NIAGMN_28745 0.61 +0.1 51,125 + traX NIAGMN_28745 0.61 +0.3 51,126 - traX NIAGMN_28745 0.61 +0.7 51,126 - traX NIAGMN_28745 0.61 +0.3 51,167 + traX NIAGMN_28745 0.66 -0.6 51,167 + traX NIAGMN_28745 0.66 +0.2 51,168 - traX NIAGMN_28745 0.66 -0.1 51,285 + traX NIAGMN_28745 0.82 -0.7 51,286 - traX NIAGMN_28745 0.82 -0.3 51,389 + +0.0 51,389 + +0.1 51,389 + +2.9 51,389 + +0.3 51,389 + -0.0 51,390 - -0.2 51,390 - -0.2 51,407 + -0.3 51,437 + -0.4 51,437 + +0.7 51,437 + -0.2 51,438 - -0.2 51,438 - -0.5 51,483 + -1.9 51,574 + NIAGMN_28750 0.11 -0.4 51,574 + NIAGMN_28750 0.11 +0.8 51,575 - NIAGMN_28750 0.11 -0.6 51,575 - NIAGMN_28750 0.11 +1.2 51,575 - NIAGMN_28750 0.11 -0.9 51,699 + NIAGMN_28750 0.21 -0.2 51,770 - NIAGMN_28750 0.26 -1.8 51,918 + NIAGMN_28750 0.38 +0.8 51,919 - NIAGMN_28750 0.38 -1.3 51,919 - NIAGMN_28750 0.38 -1.1 51,948 + NIAGMN_28750 0.40 +1.5 51,948 + NIAGMN_28750 0.40 -1.6 51,949 - NIAGMN_28750 0.41 +0.0 51,949 - NIAGMN_28750 0.41 -0.2 51,949 - NIAGMN_28750 0.41 +0.1 51,949 - NIAGMN_28750 0.41 +0.9 51,949 - NIAGMN_28750 0.41 +0.7 51,951 + NIAGMN_28750 0.41 +0.1 51,952 - NIAGMN_28750 0.41 +1.0 52,133 + NIAGMN_28750 0.55 -0.7 52,133 + NIAGMN_28750 0.55 +0.4 52,133 + NIAGMN_28750 0.55 +0.3 52,133 + NIAGMN_28750 0.55 +0.5 52,133 + NIAGMN_28750 0.55 +0.2 52,133 + NIAGMN_28750 0.55 -1.6 52,134 - NIAGMN_28750 0.55 +0.3 52,134 - NIAGMN_28750 0.55 +1.9 52,134 - NIAGMN_28750 0.55 +0.3 52,134 - NIAGMN_28750 0.55 -1.4 52,134 - NIAGMN_28750 0.55 -0.7 52,134 - NIAGMN_28750 0.55 -0.4 52,134 - NIAGMN_28750 0.55 +0.4 52,134 - NIAGMN_28750 0.55 +0.3 52,145 + NIAGMN_28750 0.56 -0.5 52,145 + NIAGMN_28750 0.56 -1.2 52,146 - NIAGMN_28750 0.56 -1.0 52,146 - NIAGMN_28750 0.56 -0.4 52,146 - NIAGMN_28750 0.56 -0.0 52,149 + NIAGMN_28750 0.56 -0.1 52,149 + NIAGMN_28750 0.56 +0.3 52,149 + NIAGMN_28750 0.56 +0.6 52,150 - NIAGMN_28750 0.57 -0.3 52,164 + NIAGMN_28750 0.58 -1.0 52,164 + NIAGMN_28750 0.58 -0.8 52,164 + NIAGMN_28750 0.58 +1.8 52,373 + NIAGMN_28750 0.74 -1.4 52,374 - NIAGMN_28750 0.74 -0.6 52,374 - NIAGMN_28750 0.74 -2.5 52,374 - NIAGMN_28750 0.74 -1.6 52,396 - NIAGMN_28750 0.76 +0.3 52,396 - NIAGMN_28750 0.76 -2.7 52,412 + NIAGMN_28750 0.77 -0.2 52,412 + NIAGMN_28750 0.77 +1.2 52,412 + NIAGMN_28750 0.77 +1.5 52,413 - NIAGMN_28750 0.77 -2.1 52,413 - NIAGMN_28750 0.77 +0.6 52,456 - NIAGMN_28750 0.81 +0.3 52,518 - NIAGMN_28750 0.86 +1.4 52,518 - NIAGMN_28750 0.86 +0.8 52,518 - NIAGMN_28750 0.86 -1.1 52,530 + NIAGMN_28750 0.87 +1.0 52,530 + NIAGMN_28750 0.87 -0.1 52,530 + NIAGMN_28750 0.87 +0.5 52,530 + NIAGMN_28750 0.87 +1.2 52,531 - NIAGMN_28750 0.87 -0.0 52,531 - NIAGMN_28750 0.87 +1.2 52,643 + -0.3 52,643 + -0.7 52,643 + -0.6 52,643 + -0.6 52,643 + -0.2 52,644 - -0.3 52,644 - -0.9 52,644 - -0.3 52,767 + -0.4 52,789 + -0.2 52,813 - +0.2 53,152 + insE NIAGMN_28755 0.53 -0.8 53,185 + insE NIAGMN_28755 0.60 -0.5 53,186 - insE NIAGMN_28755 0.60 +1.4 53,186 - insE NIAGMN_28755 0.60 -1.3 53,186 - insE NIAGMN_28755 0.60 -0.1 53,186 - insE NIAGMN_28755 0.60 -1.4 53,186 - insE NIAGMN_28755 0.60 +0.9 53,269 + insE NIAGMN_28755 0.76 +0.6 53,270 - insE NIAGMN_28755 0.77 +2.1 53,308 - insE NIAGMN_28755 0.84 +0.7 53,382 - -0.3 53,382 - +0.0 53,382 - -0.6 53,382 - -0.5 53,382 - -0.3 53,382 - +1.0 53,382 - +0.6 53,382 - -1.4 53,382 - -0.9 53,382 - +1.1 53,603 + -1.4 53,604 - -0.9 53,604 - +0.1 53,604 - -0.2 53,604 - +1.2 53,604 - +0.9 53,604 - -0.3 53,640 - -0.3 53,645 + -2.0 53,646 - -0.4 53,679 + -0.5
Or see this region's nucleotide sequence