Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27680

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntTyr_trna and Tyr_trna are separated by 209 nucleotidesTyr_trna and narI are separated by 539 nucleotidesnarI and narJ overlap by 1 nucleotidesnarJ and narH overlap by 4 nucleotides NIAGMN_27670: Tyr_trna - tRNA-Tyr(gta), at 5,361,123 to 5,361,207 _trna NIAGMN_27675: Tyr_trna - tRNA-Tyr(gta), at 5,361,417 to 5,361,501 _trna NIAGMN_27680: narI - respiratory nitrate reductase subunit gamma, at 5,362,041 to 5,362,718 narI NIAGMN_27685: narJ - nitrate reductase molybdenum cofactor assembly chaperone, at 5,362,718 to 5,363,428 narJ NIAGMN_27690: narH - nitrate reductase subunit beta, at 5,363,425 to 5,364,963 narH Position (kb) 5362 5363Strain fitness (log2 ratio) -1 0 1at 5361.363 kb on + strandat 5361.585 kb on - strandat 5361.930 kb on - strandat 5361.930 kb on - strandat 5362.070 kb on + strandat 5362.071 kb on - strandat 5362.269 kb on - strand, within narIat 5362.688 kb on + strandat 5363.583 kb on + strand, within narHat 5363.627 kb on + strand, within narHat 5363.627 kb on + strand, within narHat 5363.634 kb on - strand, within narH

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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5,361,363 + -0.8
5,361,585 - +0.4
5,361,930 - -0.9
5,361,930 - -0.7
5,362,070 + +0.7
5,362,071 - +1.6
5,362,269 - narI NIAGMN_27680 0.34 +0.5
5,362,688 + +0.1
5,363,583 + narH NIAGMN_27690 0.10 +0.6
5,363,627 + narH NIAGMN_27690 0.13 +0.3
5,363,627 + narH NIAGMN_27690 0.13 +0.6
5,363,634 - narH NIAGMN_27690 0.14 -0.1

Or see this region's nucleotide sequence