Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26665

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolC and adeB are separated by 22 nucleotidesadeB and acrA overlap by 1 nucleotides NIAGMN_26660: tolC - multidrug transporter, at 5,187,974 to 5,189,347 tolC NIAGMN_26665: adeB - hydrophobe/amphiphile efflux-1 family RND transporter, at 5,189,370 to 5,192,453 adeB NIAGMN_26670: acrA - efflux transporter periplasmic adaptor subunit, at 5,192,453 to 5,193,574 acrA Position (kb) 5189 5190 5191 5192 5193Strain fitness (log2 ratio) -1 0 1at 5188.568 kb on + strand, within tolCat 5189.084 kb on - strand, within tolCat 5189.084 kb on - strand, within tolCat 5189.348 kb on - strandat 5189.712 kb on + strand, within adeBat 5189.713 kb on - strand, within adeBat 5190.285 kb on - strand, within adeBat 5190.561 kb on - strand, within adeBat 5190.874 kb on + strand, within adeBat 5191.410 kb on + strand, within adeBat 5191.533 kb on - strand, within adeBat 5192.306 kb on - strandat 5192.306 kb on - strandat 5192.394 kb on - strandat 5192.961 kb on + strand, within acrAat 5193.356 kb on - strand, within acrA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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5,188,568 + tolC NIAGMN_26660 0.43 -1.2
5,189,084 - tolC NIAGMN_26660 0.81 -1.4
5,189,084 - tolC NIAGMN_26660 0.81 +1.0
5,189,348 - -0.4
5,189,712 + adeB NIAGMN_26665 0.11 -0.0
5,189,713 - adeB NIAGMN_26665 0.11 +1.6
5,190,285 - adeB NIAGMN_26665 0.30 +0.6
5,190,561 - adeB NIAGMN_26665 0.39 -0.7
5,190,874 + adeB NIAGMN_26665 0.49 +0.8
5,191,410 + adeB NIAGMN_26665 0.66 -0.7
5,191,533 - adeB NIAGMN_26665 0.70 -1.2
5,192,306 - +0.4
5,192,306 - +1.0
5,192,394 - -0.1
5,192,961 + acrA NIAGMN_26670 0.45 -1.1
5,193,356 - acrA NIAGMN_26670 0.80 -0.1

Or see this region's nucleotide sequence