Strain Fitness in Escherichia coli ECRC102 around NIAGMN_24910

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nticd and int are separated by 113 nucleotidesint and xis overlap by 11 nucleotidesxis and NIAGMN_24920 are separated by 103 nucleotides NIAGMN_24905: icd - NADP-dependent isocitrate dehydrogenase, at 4,850,593 to 4,851,843 icd NIAGMN_24910: int - Integrase, at 4,851,957 to 4,853,099 int NIAGMN_24915: xis - Excisionase, at 4,853,089 to 4,853,325 xis NIAGMN_24920: NIAGMN_24920 - replication protein, at 4,853,429 to 4,854,253 _24920 Position (kb) 4851 4852 4853 4854Strain fitness (log2 ratio) -1 0 1at 4851.841 kb on + strandat 4851.848 kb on + strandat 4851.848 kb on + strandat 4852.221 kb on - strand, within intat 4853.315 kb on - strandat 4853.378 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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4,851,841 + +0.3
4,851,848 + -0.5
4,851,848 + +0.1
4,852,221 - int NIAGMN_24910 0.23 +0.7
4,853,315 - +0.1
4,853,378 + -1.5

Or see this region's nucleotide sequence