Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgM and flgA are separated by 75 nucleotides flgA and flgB are separated by 154 nucleotides flgB and flgC are separated by 3 nucleotides flgC and flgD are separated by 11 nucleotides
NIAGMN_24145: flgM - anti-sigma-28 factor FlgM, at 4,722,721 to 4,723,014
flgM
NIAGMN_24150: flgA - flagellar basal body P-ring formation protein FlgA, at 4,723,090 to 4,723,749
flgA
NIAGMN_24155: flgB - flagellar basal body rod protein FlgB, at 4,723,904 to 4,724,320
flgB
NIAGMN_24160: flgC - flagellar basal body rod protein FlgC, at 4,724,324 to 4,724,728
flgC
NIAGMN_24165: flgD - flagellar hook assembly protein FlgD, at 4,724,740 to 4,725,435
flgD
Position (kb) 4723
4724
4725 Strain fitness (log2 ratio) -2
-1
0
1 at 4723.592 kb on - strand, within flgA at 4723.592 kb on - strand, within flgA at 4723.634 kb on - strand, within flgA at 4723.835 kb on - strand at 4723.867 kb on - strand at 4723.903 kb on + strand at 4724.304 kb on + strand at 4724.800 kb on - strand at 4725.274 kb on + strand, within flgD at 4725.275 kb on - strand, within flgD at 4725.275 kb on - strand, within flgD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 4,723,592 - flgA NIAGMN_24150 0.76 -0.3 4,723,592 - flgA NIAGMN_24150 0.76 +0.7 4,723,634 - flgA NIAGMN_24150 0.82 +0.7 4,723,835 - -0.8 4,723,867 - +0.6 4,723,903 + -1.1 4,724,304 + -0.3 4,724,800 - -1.8 4,725,274 + flgD NIAGMN_24165 0.77 +1.1 4,725,275 - flgD NIAGMN_24165 0.77 +0.1 4,725,275 - flgD NIAGMN_24165 0.77 -0.7
Or see this region's nucleotide sequence