Strain Fitness in Escherichia coli ECRC102 around NIAGMN_24015

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcsgA and csgC are separated by 58 nucleotidescsgC and NIAGMN_24010 are separated by 120 nucleotidesNIAGMN_24010 and ymdB are separated by 94 nucleotidesymdB and clsC overlap by 59 nucleotides NIAGMN_24000: csgA - curlin major subunit CsgA, at 4,697,328 to 4,697,786 csgA NIAGMN_24005: csgC - curli assembly protein CsgC, at 4,697,845 to 4,698,177 csgC NIAGMN_24010: NIAGMN_24010 - Uncharacterized protein YmdA, at 4,698,298 to 4,698,609 _24010 NIAGMN_24015: ymdB - O-acetyl-ADP-ribose deacetylase, at 4,698,704 to 4,699,237 ymdB NIAGMN_24020: clsC - cardiolipin synthase ClsC, at 4,699,179 to 4,700,660 clsC Position (kb) 4698 4699 4700Strain fitness (log2 ratio) -2 -1 0 1 2at 4697.723 kb on + strand, within csgAat 4697.784 kb on + strandat 4697.787 kb on + strandat 4697.787 kb on + strandat 4697.798 kb on + strandat 4697.856 kb on - strandat 4697.917 kb on + strand, within csgCat 4697.918 kb on - strand, within csgCat 4697.918 kb on - strand, within csgCat 4697.918 kb on - strand, within csgCat 4698.291 kb on - strandat 4698.604 kb on - strandat 4698.608 kb on - strandat 4698.692 kb on + strandat 4698.715 kb on + strandat 4699.040 kb on + strand, within ymdBat 4699.040 kb on + strand, within ymdBat 4699.041 kb on - strand, within ymdBat 4699.041 kb on - strand, within ymdBat 4699.043 kb on - strand, within ymdBat 4699.166 kb on + strand, within ymdBat 4699.178 kb on + strand, within ymdBat 4699.387 kb on - strand, within clsCat 4699.511 kb on - strand, within clsCat 4699.511 kb on - strand, within clsCat 4699.932 kb on - strand, within clsC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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4,697,723 + csgA NIAGMN_24000 0.86 +0.7
4,697,784 + +0.1
4,697,787 + -1.1
4,697,787 + -0.1
4,697,798 + +0.3
4,697,856 - -1.0
4,697,917 + csgC NIAGMN_24005 0.22 -0.3
4,697,918 - csgC NIAGMN_24005 0.22 +1.7
4,697,918 - csgC NIAGMN_24005 0.22 +2.1
4,697,918 - csgC NIAGMN_24005 0.22 +0.1
4,698,291 - -0.5
4,698,604 - -0.3
4,698,608 - +1.6
4,698,692 + +0.8
4,698,715 + +0.1
4,699,040 + ymdB NIAGMN_24015 0.63 -0.7
4,699,040 + ymdB NIAGMN_24015 0.63 -0.3
4,699,041 - ymdB NIAGMN_24015 0.63 +0.4
4,699,041 - ymdB NIAGMN_24015 0.63 +2.2
4,699,043 - ymdB NIAGMN_24015 0.63 +1.7
4,699,166 + ymdB NIAGMN_24015 0.87 -2.4
4,699,178 + ymdB NIAGMN_24015 0.89 -2.2
4,699,387 - clsC NIAGMN_24020 0.14 -0.7
4,699,511 - clsC NIAGMN_24020 0.22 +0.7
4,699,511 - clsC NIAGMN_24020 0.22 +0.2
4,699,932 - clsC NIAGMN_24020 0.51 -0.3

Or see this region's nucleotide sequence