Strain Fitness in Escherichia coli ECRC102 around NIAGMN_22455

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisB and hisC overlap by 1 nucleotideshisC and hisD overlap by 4 nucleotides NIAGMN_22450: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 4,433,772 to 4,434,839 hisB NIAGMN_22455: hisC - histidinol-phosphate transaminase, at 4,434,839 to 4,435,909 hisC NIAGMN_22460: hisD - Histidinol dehydrogenase, at 4,435,906 to 4,437,210 hisD Position (kb) 4434 4435 4436Strain fitness (log2 ratio) -1 0 1 2at 4433.980 kb on + strand, within hisBat 4433.997 kb on - strand, within hisBat 4434.167 kb on - strand, within hisBat 4434.258 kb on - strand, within hisBat 4434.463 kb on + strand, within hisBat 4434.463 kb on + strand, within hisBat 4434.464 kb on - strand, within hisBat 4434.586 kb on - strand, within hisBat 4434.604 kb on - strand, within hisBat 4434.858 kb on - strandat 4434.902 kb on - strandat 4434.921 kb on - strandat 4434.948 kb on - strand, within hisCat 4434.948 kb on - strand, within hisCat 4434.977 kb on - strand, within hisCat 4435.235 kb on - strand, within hisCat 4435.351 kb on - strand, within hisCat 4435.459 kb on - strand, within hisCat 4435.582 kb on - strand, within hisCat 4435.582 kb on - strand, within hisCat 4435.894 kb on + strandat 4436.382 kb on + strand, within hisDat 4436.823 kb on - strand, within hisDat 4436.823 kb on - strand, within hisD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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4,433,980 + hisB NIAGMN_22450 0.19 +0.1
4,433,997 - hisB NIAGMN_22450 0.21 +0.1
4,434,167 - hisB NIAGMN_22450 0.37 +1.1
4,434,258 - hisB NIAGMN_22450 0.46 -0.8
4,434,463 + hisB NIAGMN_22450 0.65 +2.3
4,434,463 + hisB NIAGMN_22450 0.65 -0.7
4,434,464 - hisB NIAGMN_22450 0.65 +0.1
4,434,586 - hisB NIAGMN_22450 0.76 -1.4
4,434,604 - hisB NIAGMN_22450 0.78 +0.2
4,434,858 - +1.3
4,434,902 - -1.7
4,434,921 - +1.4
4,434,948 - hisC NIAGMN_22455 0.10 +0.1
4,434,948 - hisC NIAGMN_22455 0.10 -0.1
4,434,977 - hisC NIAGMN_22455 0.13 -1.7
4,435,235 - hisC NIAGMN_22455 0.37 +1.6
4,435,351 - hisC NIAGMN_22455 0.48 +0.6
4,435,459 - hisC NIAGMN_22455 0.58 -0.9
4,435,582 - hisC NIAGMN_22455 0.69 -1.2
4,435,582 - hisC NIAGMN_22455 0.69 +0.6
4,435,894 + +0.3
4,436,382 + hisD NIAGMN_22460 0.36 +1.6
4,436,823 - hisD NIAGMN_22460 0.70 +1.5
4,436,823 - hisD NIAGMN_22460 0.70 -0.4

Or see this region's nucleotide sequence