Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pbpG and dld are separated by 37 nucleotides dld and bglX are separated by 225 nucleotides
NIAGMN_21410: pbpG - D-alanyl-D-alanine endopeptidase, at 4,246,575 to 4,247,507
pbpG
NIAGMN_21415: dld - D-lactate dehydrogenase, at 4,247,545 to 4,249,260
dld
NIAGMN_21420: bglX - beta-glucosidase BglX, at 4,249,486 to 4,251,753
bglX
Position (kb)
4247
4248
4249
4250 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4246.597 kb on + strand at 4246.775 kb on + strand, within pbpG at 4246.786 kb on + strand, within pbpG at 4246.919 kb on + strand, within pbpG at 4247.155 kb on + strand, within pbpG at 4247.156 kb on - strand, within pbpG at 4247.159 kb on + strand, within pbpG at 4247.175 kb on - strand, within pbpG at 4247.199 kb on + strand, within pbpG at 4247.201 kb on + strand, within pbpG at 4247.222 kb on + strand, within pbpG at 4247.223 kb on - strand, within pbpG at 4247.223 kb on - strand, within pbpG at 4247.276 kb on + strand, within pbpG at 4247.321 kb on + strand, within pbpG at 4247.322 kb on - strand, within pbpG at 4247.341 kb on + strand, within pbpG at 4247.364 kb on + strand, within pbpG at 4247.505 kb on + strand at 4247.575 kb on - strand at 4247.584 kb on - strand at 4247.602 kb on - strand at 4247.628 kb on - strand at 4247.656 kb on - strand at 4247.658 kb on - strand at 4247.658 kb on - strand at 4247.674 kb on - strand at 4247.674 kb on - strand at 4247.784 kb on - strand, within dld at 4247.853 kb on - strand, within dld at 4248.030 kb on - strand, within dld at 4248.098 kb on + strand, within dld at 4248.333 kb on + strand, within dld at 4248.333 kb on + strand, within dld at 4248.333 kb on + strand, within dld at 4248.333 kb on + strand, within dld at 4248.333 kb on + strand, within dld at 4248.333 kb on + strand, within dld at 4248.334 kb on - strand, within dld at 4248.335 kb on + strand, within dld at 4248.335 kb on + strand, within dld at 4248.533 kb on + strand, within dld at 4248.578 kb on + strand, within dld at 4248.579 kb on - strand, within dld at 4248.579 kb on - strand, within dld at 4248.752 kb on + strand, within dld at 4248.789 kb on + strand, within dld at 4248.790 kb on - strand, within dld at 4248.790 kb on - strand, within dld at 4248.790 kb on - strand, within dld at 4248.888 kb on - strand, within dld at 4248.986 kb on + strand, within dld at 4249.026 kb on + strand, within dld at 4249.047 kb on + strand, within dld at 4249.097 kb on - strand at 4249.298 kb on + strand at 4249.313 kb on + strand at 4249.313 kb on + strand at 4249.313 kb on + strand at 4249.314 kb on - strand at 4249.314 kb on - strand at 4249.314 kb on - strand at 4249.400 kb on - strand at 4249.751 kb on + strand, within bglX at 4249.986 kb on + strand, within bglX at 4249.986 kb on + strand, within bglX at 4249.990 kb on + strand, within bglX at 4249.990 kb on + strand, within bglX at 4249.991 kb on - strand, within bglX at 4249.991 kb on - strand, within bglX at 4250.038 kb on - strand, within bglX at 4250.102 kb on + strand, within bglX at 4250.119 kb on + strand, within bglX at 4250.119 kb on + strand, within bglX at 4250.120 kb on - strand, within bglX at 4250.162 kb on - strand, within bglX at 4250.163 kb on + strand, within bglX at 4250.163 kb on + strand, within bglX at 4250.163 kb on + strand, within bglX at 4250.164 kb on - strand, within bglX
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 4,246,597 + -0.5 4,246,775 + pbpG NIAGMN_21410 0.21 +0.6 4,246,786 + pbpG NIAGMN_21410 0.23 -0.9 4,246,919 + pbpG NIAGMN_21410 0.37 -0.9 4,247,155 + pbpG NIAGMN_21410 0.62 +0.7 4,247,156 - pbpG NIAGMN_21410 0.62 +1.1 4,247,159 + pbpG NIAGMN_21410 0.63 +1.0 4,247,175 - pbpG NIAGMN_21410 0.64 +1.3 4,247,199 + pbpG NIAGMN_21410 0.67 +1.7 4,247,201 + pbpG NIAGMN_21410 0.67 -0.6 4,247,222 + pbpG NIAGMN_21410 0.69 +0.2 4,247,223 - pbpG NIAGMN_21410 0.69 -1.0 4,247,223 - pbpG NIAGMN_21410 0.69 +0.4 4,247,276 + pbpG NIAGMN_21410 0.75 -0.3 4,247,321 + pbpG NIAGMN_21410 0.80 -0.2 4,247,322 - pbpG NIAGMN_21410 0.80 -0.3 4,247,341 + pbpG NIAGMN_21410 0.82 +0.1 4,247,364 + pbpG NIAGMN_21410 0.85 +1.2 4,247,505 + +2.2 4,247,575 - +0.4 4,247,584 - -0.9 4,247,602 - +0.4 4,247,628 - +0.4 4,247,656 - -0.4 4,247,658 - +0.7 4,247,658 - -0.6 4,247,674 - -0.7 4,247,674 - -1.9 4,247,784 - dld NIAGMN_21415 0.14 -0.6 4,247,853 - dld NIAGMN_21415 0.18 +1.7 4,248,030 - dld NIAGMN_21415 0.28 +1.0 4,248,098 + dld NIAGMN_21415 0.32 -0.6 4,248,333 + dld NIAGMN_21415 0.46 +0.5 4,248,333 + dld NIAGMN_21415 0.46 +1.7 4,248,333 + dld NIAGMN_21415 0.46 +1.7 4,248,333 + dld NIAGMN_21415 0.46 +0.1 4,248,333 + dld NIAGMN_21415 0.46 -2.1 4,248,333 + dld NIAGMN_21415 0.46 +0.7 4,248,334 - dld NIAGMN_21415 0.46 +0.1 4,248,335 + dld NIAGMN_21415 0.46 -0.9 4,248,335 + dld NIAGMN_21415 0.46 +2.1 4,248,533 + dld NIAGMN_21415 0.58 -0.3 4,248,578 + dld NIAGMN_21415 0.60 -0.5 4,248,579 - dld NIAGMN_21415 0.60 -0.1 4,248,579 - dld NIAGMN_21415 0.60 +0.1 4,248,752 + dld NIAGMN_21415 0.70 +0.1 4,248,789 + dld NIAGMN_21415 0.72 +1.0 4,248,790 - dld NIAGMN_21415 0.73 +0.4 4,248,790 - dld NIAGMN_21415 0.73 +1.3 4,248,790 - dld NIAGMN_21415 0.73 -1.1 4,248,888 - dld NIAGMN_21415 0.78 -0.3 4,248,986 + dld NIAGMN_21415 0.84 -0.1 4,249,026 + dld NIAGMN_21415 0.86 +0.1 4,249,047 + dld NIAGMN_21415 0.88 -2.5 4,249,097 - -1.2 4,249,298 + -0.3 4,249,313 + +0.1 4,249,313 + -1.3 4,249,313 + -0.7 4,249,314 - +1.4 4,249,314 - -1.5 4,249,314 - +0.8 4,249,400 - +0.0 4,249,751 + bglX NIAGMN_21420 0.12 -0.9 4,249,986 + bglX NIAGMN_21420 0.22 -0.3 4,249,986 + bglX NIAGMN_21420 0.22 +1.1 4,249,990 + bglX NIAGMN_21420 0.22 -0.9 4,249,990 + bglX NIAGMN_21420 0.22 +1.3 4,249,991 - bglX NIAGMN_21420 0.22 +0.7 4,249,991 - bglX NIAGMN_21420 0.22 +2.4 4,250,038 - bglX NIAGMN_21420 0.24 +0.5 4,250,102 + bglX NIAGMN_21420 0.27 +0.5 4,250,119 + bglX NIAGMN_21420 0.28 -1.3 4,250,119 + bglX NIAGMN_21420 0.28 +0.7 4,250,120 - bglX NIAGMN_21420 0.28 +0.7 4,250,162 - bglX NIAGMN_21420 0.30 -0.3 4,250,163 + bglX NIAGMN_21420 0.30 +0.1 4,250,163 + bglX NIAGMN_21420 0.30 -0.0 4,250,163 + bglX NIAGMN_21420 0.30 -0.7 4,250,164 - bglX NIAGMN_21420 0.30 +1.1
Or see this region's nucleotide sequence