Strain Fitness in Escherichia coli ECRC102 around NIAGMN_16420

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnfeF and Ile2_trna are separated by 53 nucleotidesIle2_trna and mug are separated by 124 nucleotidesmug and rpoD are separated by 78 nucleotidesrpoD and dnaG are separated by 194 nucleotides NIAGMN_16405: nfeF - NADPH-dependent ferric chelate reductase, at 3,245,707 to 3,246,471 nfeF NIAGMN_16410: Ile2_trna - tRNA-Ile2(cat), at 3,246,525 to 3,246,600 _trna NIAGMN_16415: mug - G/U mismatch-specific DNA glycosylase, at 3,246,725 to 3,247,231 mug NIAGMN_16420: rpoD - RNA polymerase sigma factor RpoD, at 3,247,310 to 3,249,151 rpoD NIAGMN_16425: dnaG - DNA primase, at 3,249,346 to 3,251,091 dnaG Position (kb) 3247 3248 3249 3250Strain fitness (log2 ratio) -2 -1 0 1 2at 3246.355 kb on + strand, within nfeFat 3246.355 kb on + strand, within nfeFat 3246.356 kb on - strand, within nfeFat 3246.356 kb on - strand, within nfeFat 3246.381 kb on + strand, within nfeFat 3246.425 kb on - strandat 3246.519 kb on + strandat 3246.520 kb on - strandat 3246.520 kb on - strandat 3246.520 kb on - strandat 3246.520 kb on - strandat 3246.520 kb on - strandat 3246.520 kb on - strandat 3246.520 kb on - strandat 3246.616 kb on - strandat 3246.616 kb on - strandat 3246.616 kb on - strandat 3246.620 kb on + strandat 3246.620 kb on + strandat 3246.620 kb on + strandat 3246.691 kb on - strandat 3246.710 kb on + strandat 3246.710 kb on + strandat 3246.711 kb on - strandat 3246.711 kb on - strandat 3246.720 kb on + strandat 3246.720 kb on + strandat 3246.721 kb on - strandat 3246.724 kb on + strandat 3246.724 kb on + strandat 3246.846 kb on + strand, within mugat 3246.847 kb on - strand, within mugat 3246.847 kb on - strand, within mugat 3246.848 kb on + strand, within mugat 3246.848 kb on + strand, within mugat 3246.849 kb on - strand, within mugat 3246.849 kb on - strand, within mugat 3246.978 kb on + strand, within mugat 3246.978 kb on + strand, within mugat 3247.058 kb on + strand, within mugat 3249.259 kb on - strandat 3249.273 kb on - strandat 3249.273 kb on - strandat 3249.273 kb on - strandat 3249.273 kb on - strandat 3249.275 kb on - strandat 3249.275 kb on - strandat 3249.275 kb on - strandat 3249.277 kb on - strandat 3249.277 kb on - strandat 3249.277 kb on - strandat 3249.335 kb on - strandat 3249.335 kb on - strandat 3249.335 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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3,246,355 + nfeF NIAGMN_16405 0.85 +1.2
3,246,355 + nfeF NIAGMN_16405 0.85 -1.0
3,246,356 - nfeF NIAGMN_16405 0.85 +1.0
3,246,356 - nfeF NIAGMN_16405 0.85 -1.8
3,246,381 + nfeF NIAGMN_16405 0.88 +0.4
3,246,425 - +1.4
3,246,519 + -0.4
3,246,520 - -0.4
3,246,520 - -2.3
3,246,520 - -0.3
3,246,520 - -0.0
3,246,520 - -1.6
3,246,520 - +0.2
3,246,520 - +0.1
3,246,616 - -0.4
3,246,616 - +0.6
3,246,616 - +1.1
3,246,620 + -0.4
3,246,620 + -1.9
3,246,620 + -1.1
3,246,691 - -1.4
3,246,710 + +0.6
3,246,710 + -0.0
3,246,711 - -0.8
3,246,711 - -0.3
3,246,720 + -0.7
3,246,720 + -1.3
3,246,721 - -0.4
3,246,724 + +0.4
3,246,724 + -0.6
3,246,846 + mug NIAGMN_16415 0.24 +0.9
3,246,847 - mug NIAGMN_16415 0.24 +0.4
3,246,847 - mug NIAGMN_16415 0.24 -2.0
3,246,848 + mug NIAGMN_16415 0.24 -0.6
3,246,848 + mug NIAGMN_16415 0.24 -0.6
3,246,849 - mug NIAGMN_16415 0.24 +0.4
3,246,849 - mug NIAGMN_16415 0.24 -0.6
3,246,978 + mug NIAGMN_16415 0.50 -0.7
3,246,978 + mug NIAGMN_16415 0.50 -0.4
3,247,058 + mug NIAGMN_16415 0.66 -0.6
3,249,259 - +1.0
3,249,273 - -1.0
3,249,273 - +1.0
3,249,273 - -0.3
3,249,273 - -1.5
3,249,275 - +1.0
3,249,275 - +2.0
3,249,275 - -2.2
3,249,277 - -0.8
3,249,277 - -0.8
3,249,277 - -2.0
3,249,335 - -0.6
3,249,335 - +0.8
3,249,335 - +0.4

Or see this region's nucleotide sequence