Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pulO and rpsJ are separated by 378 nucleotides rpsJ and rplC are separated by 32 nucleotides rplC and rplD are separated by 10 nucleotides rplD and rplW overlap by 4 nucleotides rplW and rplB are separated by 17 nucleotides
NIAGMN_15120: pulO - prepilin peptidase, at 3,012,601 to 3,013,068
pulO
NIAGMN_15125: rpsJ - 30S ribosomal protein S10, at 3,013,447 to 3,013,758
rpsJ
NIAGMN_15130: rplC - 50S ribosomal protein L3, at 3,013,791 to 3,014,420
rplC
NIAGMN_15135: rplD - 50S ribosomal protein L4, at 3,014,431 to 3,015,036
rplD
NIAGMN_15140: rplW - 50S ribosomal protein L23, at 3,015,033 to 3,015,335
rplW
NIAGMN_15145: rplB - 50S ribosomal protein L2, at 3,015,353 to 3,016,174
rplB
Position (kb)
3013
3014
3015 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3012.818 kb on + strand, within pulO at 3012.819 kb on - strand, within pulO at 3012.824 kb on - strand, within pulO at 3012.824 kb on - strand, within pulO at 3012.834 kb on + strand, within pulO at 3012.845 kb on - strand, within pulO at 3012.851 kb on + strand, within pulO at 3012.861 kb on - strand, within pulO at 3012.861 kb on - strand, within pulO at 3012.861 kb on - strand, within pulO at 3012.888 kb on + strand, within pulO at 3012.888 kb on + strand, within pulO at 3012.888 kb on + strand, within pulO at 3012.889 kb on - strand, within pulO at 3012.889 kb on - strand, within pulO at 3012.938 kb on + strand, within pulO at 3012.938 kb on + strand, within pulO at 3012.939 kb on - strand, within pulO at 3012.939 kb on - strand, within pulO at 3012.939 kb on - strand, within pulO at 3012.962 kb on - strand, within pulO at 3012.972 kb on + strand, within pulO at 3012.972 kb on + strand, within pulO at 3012.973 kb on - strand, within pulO at 3012.973 kb on - strand, within pulO at 3012.973 kb on - strand, within pulO at 3012.973 kb on - strand, within pulO at 3012.985 kb on + strand, within pulO at 3012.985 kb on + strand, within pulO at 3012.986 kb on - strand, within pulO at 3013.018 kb on + strand, within pulO at 3013.019 kb on - strand, within pulO at 3013.037 kb on + strand at 3013.037 kb on + strand at 3013.060 kb on - strand at 3013.068 kb on + strand at 3013.068 kb on + strand at 3013.069 kb on - strand at 3013.069 kb on - strand at 3013.082 kb on + strand at 3013.083 kb on - strand at 3013.091 kb on + strand at 3013.105 kb on - strand at 3013.182 kb on + strand at 3013.183 kb on - strand at 3013.334 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 3,012,818 + pulO NIAGMN_15120 0.46 +1.7 3,012,819 - pulO NIAGMN_15120 0.47 +0.5 3,012,824 - pulO NIAGMN_15120 0.48 +0.7 3,012,824 - pulO NIAGMN_15120 0.48 -0.5 3,012,834 + pulO NIAGMN_15120 0.50 +0.6 3,012,845 - pulO NIAGMN_15120 0.52 -0.4 3,012,851 + pulO NIAGMN_15120 0.53 -0.8 3,012,861 - pulO NIAGMN_15120 0.56 +0.0 3,012,861 - pulO NIAGMN_15120 0.56 +0.8 3,012,861 - pulO NIAGMN_15120 0.56 -0.2 3,012,888 + pulO NIAGMN_15120 0.61 +0.0 3,012,888 + pulO NIAGMN_15120 0.61 +0.8 3,012,888 + pulO NIAGMN_15120 0.61 +1.3 3,012,889 - pulO NIAGMN_15120 0.62 -0.1 3,012,889 - pulO NIAGMN_15120 0.62 +1.4 3,012,938 + pulO NIAGMN_15120 0.72 -1.1 3,012,938 + pulO NIAGMN_15120 0.72 +0.4 3,012,939 - pulO NIAGMN_15120 0.72 +0.3 3,012,939 - pulO NIAGMN_15120 0.72 +2.3 3,012,939 - pulO NIAGMN_15120 0.72 -1.1 3,012,962 - pulO NIAGMN_15120 0.77 -1.7 3,012,972 + pulO NIAGMN_15120 0.79 +0.0 3,012,972 + pulO NIAGMN_15120 0.79 +0.6 3,012,973 - pulO NIAGMN_15120 0.79 +1.6 3,012,973 - pulO NIAGMN_15120 0.79 -0.8 3,012,973 - pulO NIAGMN_15120 0.79 +0.1 3,012,973 - pulO NIAGMN_15120 0.79 -0.1 3,012,985 + pulO NIAGMN_15120 0.82 +0.4 3,012,985 + pulO NIAGMN_15120 0.82 -0.0 3,012,986 - pulO NIAGMN_15120 0.82 -1.8 3,013,018 + pulO NIAGMN_15120 0.89 +0.9 3,013,019 - pulO NIAGMN_15120 0.89 -0.3 3,013,037 + -1.8 3,013,037 + -0.2 3,013,060 - +1.4 3,013,068 + +0.0 3,013,068 + -0.5 3,013,069 - -0.9 3,013,069 - +0.2 3,013,082 + -0.3 3,013,083 - -0.1 3,013,091 + +1.0 3,013,105 - +0.6 3,013,182 + +0.1 3,013,183 - -0.4 3,013,334 + +1.0
Or see this region's nucleotide sequence